Bone marrow mesenchymal stem cells promote head and neck cancer progression through Periostin‐mediated phosphoinositide 3‐kinase/<scp>Akt</scp>/mammalian target of rapamycin

Liu, Chuanxia; Feng, Xiaoxia; Wang, Baixiang; Wang, Xinhua; Wang, Chaowei; Yu, Mengfei; Cao, Guifen; Wang, Huiming

doi:10.1111/cas.13479

Cited by 57 publications

(51 citation statements)

References 36 publications

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“…Similar to our results, they did not observe signi cant effects of hDPSC-derived conditioned medium (CM) on in vitro HNSCC proliferation and therapeutic sensitivity, and in vivo tumour growth, despite the increase in tumour VEGF secretion(45). Con icting results on the impact of other MSC subtypes on in vitro and in vivo HNC cell proliferation, survival, migration, invasion and therapeutic sensitivity have previously been described(46)(47)(48)(49)(50)(51)(52)(53). These effects are mediated in a direct way via differentiation ofMSCs into malignant cells (54, 55), cancer-associated broblasts (CAFs) (56) and vascular-related cells (57, 58), or indirectly by (paracrine) interaction with immune cells (27, 59, 60), cancer stem cells (CSCs) (61-63), endothelial cells (24, 28, 64) and tumour cells (24, 30, 31, 65).…”

supporting

confidence: 89%

Safety and Homing of Human Dental Pulp Stromal Cells in Head and Neck Cancer

Merckx

Monaco

Lambrichts

et al. 2020

Preprint

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Background: Head and neck cancer (HNC) is one of the most common cancers, associated with a huge mortality and morbidity. In order to improve patient outcomes, more efficient and targeted therapies are essential. Bone marrow-derived mesenchymal stromal cells (BM-MSCs) express tumour homing capacity, which could be exploited to target anti-cancer drug delivery to the tumour region and reduce adverse side-effects. Nevertheless, dental pulp stromal cells (DPSCs), an MSC-like population present in teeth, could offer important clinical benefits because of their easy isolation and superior proliferation compared to BM-MSCs. Therefore, we aimed to elucidate the tumour homing and safe usage of DPSCs to treat HNC. Methods: The in vivo survival as well as the effect of intratumourally administered DPSCs on tumour aggressiveness was tested in a HNC xenograft mouse model by using bioluminescence imaging (BLI), (immuno)histology and qRT-PCR. Furthermore, the in vitro and in vivo tumour homing capacity of DPSCs towards a HNC cell line were evaluated by a transwell migration assay and BLI, respectively. Results: Intratumourally injected DPSCs survived for at least two weeks in the tumour micro-environment and had no significant influence on tumour morphology, growth, angiogenesis and epithelial-to-mesenchymal transition. In addition, DPSCs migrated towards tumour cells in vitro, which could not be confirmed after their in vivo intravenous, intraperitoneal or peritumoural injection under the tested experimental conditions. Conclusions: Our research suggests that intratumourally delivered DPSCs might be used as safe factories for the continuous delivery of anti-cancer drugs in HNC. Nevertheless, further optimization as well as efficacy studies are necessary to understand and improve in vivo tumour homing and determine the optimal experimental set-up of stem cell-based cancer therapies, including dosing and timing.

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supporting

confidence: 89%

Safety and Homing of Human Dental Pulp Stromal Cells in Head and Neck Cancer

Merckx

Monaco

Lambrichts

et al. 2020

Preprint

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“…Additionally, other ILs and cytokines secreted by MSCs promote tumor growth in different solid tumors and hematological malignancies (17,48). For example, in the conditioned medium of MSCs, periostin was found to promote the proliferation of head and neck cancer cells (49). On the other hand, IL-1β from the conditioned medium of FaDu cells mediates a proinflammatory and possibly protumorigenic response in MSCs (50).…”

Section: Discussionmentioning

confidence: 99%

Interaction of head and neck squamous cell carcinoma cells and mesenchymal stem cells under hypoxia and normoxia

et al. 2020

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Mesenchymal stem cells (MSCs) exhibit strong tropism towards tumor tissue. While MSCs generally surround tumors, they can also infiltrate tumors and thereby influence their proliferation. Interactions between MSCs and tumor cells are usually tested under normoxia, but the majority of solid tumors, including head and neck squamous cell carcinoma (HNSCC), are also characterized by hypoxic areas. Hence, the present study aimed to assess the interaction between MSCs and tumor cells under hypoxic conditions. MSCs were cultivated under normoxia and hypoxia, and conditioned media were used to cultivate the HNSCC cell line FaDu. The cell cycle distribution and viability of MSCs and the proliferation of FaDu cells were analyzed under normoxia and hypoxia, and changes in cytokine levels in the conditioned media were evaluated. No cell cycle changes were observed for MSCs after 24 h of cultivation under hypoxia, but the cell viability had declined. Hypoxia also led to a decrease in the proliferation of FaDu cells; however, FaDu cells proliferated faster after 48 h under hypoxia compared with normoxic conditions. This effect was reversed after incubation under normoxia for 72 h and hypoxia for 72 h. While these changes constituted a trend, these differences were not statistically significant. A cytokine assay showed an increase in interleukin (IL)-6 in the hypoxic medium. Overall, the results indicated that there was an interaction between MSCs and tumor cells. The presence or absence of oxygen seemed to influence the functionality of MSCs and their protumorigenic properties, in which IL-6 was identified as a potential mediator. Since MSCs are a component of the tumor stroma, further in vitro and in vivo studies are needed to investigate this interaction in order to develop novel approaches for tumor therapy.

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“…Stem cells and cancer cells have diverse interactions based on the histological origin of the latter. While the cancer-promoting effect of stem cells has been observed in malignancies such as breast cancer[ 69 ] as well as head and neck cancers,[ 70 ] the existing evidence has generally supported the use of transplanted NSCs and MSCs as safe therapeutic platforms to treat gliomas. Several recent studies have suggested their inhibitory effects against gliomas, such as the reported study of NSCs directly inhibiting the invasion and proliferation of gliomas[ 71 ] and their association with a survival benefit.…”

Section: B Asic R Ationale For mentioning

confidence: 99%

Imaging Gliomas with Nanoparticle-Labeled Stem Cells

Deng

Zhao

2018

Chinese Medical Journal

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Objective:Gliomas are the most common neoplasm of the central nervous system (CNS); however, traditional imaging techniques do not show the boundaries of tumors well. Some researchers have found a new therapeutic mode to combine nanoparticles, which are nanosized particles with various properties for specific therapeutic purposes, and stem cells for tracing gliomas. This review provides an introduction of the basic understanding and clinical applications of the combination of stem cells and nanoparticles as a contrast agent for glioma imaging.Data Sources:Studies published in English up to and including 2017 were extracted from the PubMed database with the selected key words of “stem cell,” “glioma,” “nanoparticles,” “MRI,” “nuclear imaging,” and “Fluorescence imaging.”Study Selection:The selection of studies focused on both preclinical studies and basic studies of tracking glioma with nanoparticle-labeled stem cells.Results:Studies have demonstrated successful labeling of stem cells with multiple types of nanoparticles. These labeled stem cells efficiently migrated to gliomas of varies models and produced signals sensitively captured by different imaging modalities.Conclusion:The use of nanoparticle-labeled stem cells is a promising imaging platform for the tracking and treatment of gliomas.

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Bone marrow mesenchymal stem cells promote head and neck cancer progression through Periostin‐mediated phosphoinositide 3‐kinase/Akt/mammalian target of rapamycin

Cited by 57 publications

References 36 publications

Safety and Homing of Human Dental Pulp Stromal Cells in Head and Neck Cancer

Safety and Homing of Human Dental Pulp Stromal Cells in Head and Neck Cancer

Interaction of head and neck squamous cell carcinoma cells and mesenchymal stem cells under hypoxia and normoxia

Imaging Gliomas with Nanoparticle-Labeled Stem Cells

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