Bone Marrow Mesenchymal Stem Cells and Endothelial Progenitor Cells Co-Culture Enhances Large Segment Bone Defect Repair

Peng, Jing; Chen, Long; Peng, Kun; Chen, Xuewen; Wu, Jun; He, Zhiyu; Xiang, Zhou

doi:10.1166/jbn.2019.2735

Cited by 37 publications

(26 citation statements)

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“…Vascularization is a prerequisite for tissue repair, which provides su cient blood supply, oxygen, nutrients and growth factors. BMSCs have been reported to play an important role in vascularization, particularly due to their secretion of vesicles (26). Accumulating evidence suggests that exosome is a novel alternative to cell-based approaches due to its superiority in avoiding emboli formation, immunogenicity, and malignant transformation (27).However, the yield and activity of exosomes limit their application.…”

Section: Discussionmentioning

confidence: 99%

Exosomes From 3d Culture of Marrow Stem Cells Enhances Endothelial Cell Proliferation, Migration, and Angiogenesis via Activation of the Hmgb1/akt Pathway

Gao¹,

Tang

et al. 2020

Preprint

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BackgroundAngiogenesis is an essential step in tissue engineering. MSCexosomes play an important role in angiogenesis. Functional biomolecules in exosomes vested by the culture microenvironment can be transferred to recipient cells and affects their effect. 3D culture can improve the proliferation and activity of MSCs. However, whether exosomes derived from 3D culture of MSCs have an enhanced effect on angiogenesis is unclear. MethodsHerein, we compared the bioactivity of exosomes produced by conventional 2D culture (2D-exos) and 3D culture (3D-exos) of bone marrow stem cells (BMSCs) in angiogenesis. ResultsA series of in vitro and in vivo experiments indicated that 3D-exos exhibited stronger effects on HUVEC cell proliferation, migration, tube formation, and in vivo angiogenesis compared with 2D-exos. Moreover, the superiority of 3D-exos might be attributed to the activation of HMGB1/AKT signaling. ConclusionsThese results indicate that exosomes from 3D culture of MSCs may serve as a potential therapeutic approach for pro-angiogenesis.

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Section: Discussionmentioning

confidence: 99%

Exosomes From 3d Culture of Marrow Stem Cells Enhances Endothelial Cell Proliferation, Migration, and Angiogenesis via Activation of the Hmgb1/akt Pathway

Gao¹,

Tang

et al. 2020

Preprint

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“…Common combinations of cell types include endothelial cells and osteoblastic cells, [261][262][263] CD34 + peripheral blood cells and bone marrow-derived MSCs, 264 pericytes, and adventitial cells, 265 as well as EPCs and undifferentiated MSCs. [266][267][268][269][270] Moreover, Amini et al 269 established a double-tracked approach for bone regeneration in large defect areas in vivo, involving oxygen-controlled matrices and an optimal cell combination of MSCs and EPCs, which increases oxygen tension levels and cell viability. Scaffolds were co-cultured with MSCs and EPCs for 2 days and subsequently implanted in nude mouse subcutaneous tissue and a rabbit ulnar defect model.…”

Section: Cell-based Vascularization Strategiesmentioning

confidence: 99%

Vascularization Strategies in the Prevention of Nonunion Formation

Menger

Laschke

Orth

et al. 2021

Tissue Engineering Part B: Reviews

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Delayed healing and nonunion formation are major challenges in orthopedic surgery, which require the development of novel treatment strategies. Vascularization is considered one of the major prerequisites for successful bone healing, providing an adequate nutrient supply and allowing the infiltration of progenitor cells to the fracture site. Hence, during the last decade, a considerable number of studies have focused on the evaluation of vascularization strategies to prevent or to treat nonunion formation. These involve (1) biophysical applications, (2) systemic pharmacological interventions, and (3) tissue engineering, including sophisticated scaffold materials, local growth factor delivery systems, cell-based techniques, and surgical vascularization approaches. Accumulating evidence indicates that in nonunions, these strategies are indeed capable of improving the process of bone healing. The major challenge for the future will now be the translation of these strategies into clinical practice to make them accessible for the majority of patients. If this succeeds, these vascularization strategies may markedly reduce the incidence of nonunion formation.

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“…The occurrence of EPCs with MSCs has profound effects on cell proliferation and differentiation [21,22]. Investigations have shown that co-implantation of EPCs and MSCs significantly improves bone formation and neovascularization [23][24][25] Therefore, we used MSCs and EPCs to study their osteogenic interaction in co-culture experiments, to identify gene expression changes driving this interaction, to determine how EPCs influence osteogenic differentiation of MSCs, and to investigate the involvement of mitogen-activated protein kinase (MAPKs) pathways in the osteogenic differentiation.…”

Section: Introductionmentioning

confidence: 99%

Endothelial Progenitor Cells Induce Osteogenic Differentiation in Co-cultured Mesenchymal Stem Cells via the MAPK Signaling Pathway

Chu¹,

liu²,

he³

et al. 2020

Preprint

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Background: The role of bone tissue engineering is to regenerate tissue using biomaterials and stem cell based approaches. Combination of two or more cell types is one of the strategies to promote bone formation. Endothelial progenitor cells (EPCs) may enhance the osteogenic properties of mesenchymal stem cells (MSCs) and promote bone healing, this study aimed to investigate the possible mechanisms of EPCs on promoting osteogenic differentiation of MSCs.Methods: MSCs and EPCs were isolated and co-cultured in Transwell chambers, the effects of EPCs on the regulation of MSC biological properties was investigated. Real-time PCR array, qRT-PCR and western blotting were performed to explore possible signaling pathways involved in osteogenesis. The expression of osteogenesis markers and calcium nodule formation was quantified by qRT-PCR, western blotting and Alizarin Red staining. Results: Results showed that when co-cultured with EPCs, MSCs exhibited greater alkaline phosphatase (ALP) activity and increased calcium mineral deposition significantly. The mitogen-activated protein kinase (MAPK) signaling pathway was involved in this process. p38 gene expression and p38 protein phosphorylation levels showed significant up-regulation in co-cultured MSCs. Silence expression of p38 in co-cultured MSCs reduced osteogenic gene expression, protein synthesis, ALP activity and calcium nodule formation.Conclusions: These data suggest paracrine signaling from EPCs influence the biological function and promote MSCs osteogenic differentiation. Activation of the p38MAPK pathway may be the key to enhancing MSCs osteogenic differentiation via indirect interactions with EPCs.

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Bone Marrow Mesenchymal Stem Cells and Endothelial Progenitor Cells Co-Culture Enhances Large Segment Bone Defect Repair

Cited by 37 publications

References 0 publications

Exosomes From 3d Culture of Marrow Stem Cells Enhances Endothelial Cell Proliferation, Migration, and Angiogenesis via Activation of the Hmgb1/akt Pathway

Exosomes From 3d Culture of Marrow Stem Cells Enhances Endothelial Cell Proliferation, Migration, and Angiogenesis via Activation of the Hmgb1/akt Pathway

Vascularization Strategies in the Prevention of Nonunion Formation

Endothelial Progenitor Cells Induce Osteogenic Differentiation in Co-cultured Mesenchymal Stem Cells via the MAPK Signaling Pathway

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