Bone Marrow Stem Cell and Decellularized Human Amniotic Membrane for the Treatment of Nonhealing Wound After Radiation Therapy

Kakabadze, Zurab; Chakhunashvili, David; Gogilashvili, Ketevan; Ediberidze, Ketevan; Chakhunashvili, Konstantine; Kalandarishvili, Konstantine; Karalashvili, L

doi:10.6002/ect.mesot2018.o29

Cited by 24 publications

(29 citation statements)

References 55 publications

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“…It had been proven that the BM of hAM had great therapeutic potential in human ocular surface reconstruction [41-43], and wound healing caused by radiation [44]. Also, hAECs were used in tissue regeneration for their characteristic of pluripotency and immune privilege [12,13,45].…”

Section: Resultsmentioning

confidence: 99%

Producing Human Amniotic Epithelial Cells-only Membrane for Transplantation

Guo

Ahmed

et al. 2019

Preprint

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Human amniotic epithelial cells (hAECs), as pluripotent stem cells, have characteristics of immune privilege and great clinical potential. Here, we produced hAECs membrane consisting of single-layer hAECs and basal membrane (BM) of human amniotic membrane (hAM). In conventional methods, hAECs were isolated from hAM by repeated trypsin digestion. In this study, collagenase I and cell scraper were used to remove human amniotic mesenchymal stem cells (hAMSCs) from hAM and hAECs-only membranes were produced. These hAECs on the membranes were evaluated by surface biomarkers including epithelial cell adhesion molecule (EpCAM), stage-specific embryonic antigen 4 (SSEA4) and endoglin (CD105), transcriptional level of biomarkers including POU class 5 homeobox 1 (OCT4), sex determining region Y-box 2 (SOX2), fibroblast growth factor 4 (FGF4), immunofluorescence of cytokeratin-8 (CK-8), alpha smooth muscle actin (α-SMA) and collagen type I alpha 1 chain (ColA1). Finally, the hAECs membrane were transplanted on skin-removed mice to evaluate its effect on wound healing. In comparison to the hAECs isolated by the conventional methods, the cells isolated by this proposed method had higher purity of hAECs, expressed higher in pluripotency related genes, and maintained an epithelium construction in a long-term culture. In mice application, the hAECs membrane effectively improved the skin wound healing. An efficient method was successfully established to produce hAECs membrane in this work which not only held promise to obtain hAECs in higher purity and quality, but also showed practical clinical potential.

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Section: Resultsmentioning

confidence: 99%

Producing Human Amniotic Epithelial Cells-only Membrane for Transplantation

Guo

Ahmed

et al. 2019

Preprint

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“…Decellularized or dehydrated perinatal tissues were used as grafts or as support for perinatal cells or cells of another origin and were applied topically. In cases where the entire full-thickness or burn wound area was covered with the hAM (Kim et al, 2009;Samadikuchaksaraei et al, 2016;Motamed et al, 2017;Song et al, 2017;Farzamfar et al, 2018;Gholipourmalekabadi et al, 2018;Kakabadze et al, 2019;Arasteh et al, 2020;Nasiry et al, 2020), the wound areas are given instead of dosages (Table 4). Also, in two studies where a proprietary human amnion/chorion graft (EpiFix ® ) was implanted subcutaneously, calculating the dosages was inapplicable (Koob et al, 2013;Koob et al, 2014).…”

Section: Perinatal Tissuesmentioning

confidence: 99%

Systematic Review of the Application of Perinatal Derivatives in Animal Models on Cutaneous Wound Healing

Pichlsberger

Jerman

Obradović

et al. 2021

Front. Bioeng. Biotechnol.

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Knowledge of the beneficial effects of perinatal derivatives (PnD) in wound healing goes back to the early 1900s when the human fetal amniotic membrane served as a biological dressing to treat burns and skin ulcerations. Since the twenty-first century, isolated cells from perinatal tissues and their secretomes have gained increasing scientific interest, as they can be obtained non-invasively, have anti-inflammatory, anti-cancer, and anti-fibrotic characteristics, and are immunologically tolerated in vivo. Many studies that apply PnD in pre-clinical cutaneous wound healing models show large variations in the choice of the animal species (e.g., large animals, rodents), the choice of diabetic or non-diabetic animals, the type of injury (full-thickness wounds, burns, radiation-induced wounds, skin flaps), the source and type of PnD (placenta, umbilical cord, fetal membranes, cells, secretomes, tissue extracts), the method of administration (topical application, intradermal/subcutaneous injection, intravenous or intraperitoneal injection, subcutaneous implantation), and the type of delivery systems (e.g., hydrogels, synthetic or natural biomaterials as carriers for transplanted cells, extracts or secretomes). This review provides a comprehensive and integrative overview of the application of PnD in wound healing to assess its efficacy in preclinical animal models. We highlight the advantages and limitations of the most commonly used animal models and evaluate the impact of the type of PnD, the route of administration, and the dose of cells/secretome application in correlation with the wound healing outcome. This review is a collaborative effort from the COST SPRINT Action (CA17116), which broadly aims at approaching consensus for different aspects of PnD research, such as providing inputs for future standards for the preclinical application of PnD in wound healing.

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“…E dependo da espécie, mantém arcabouço estrutural e complexo sistema vascular. Mesmo descelularizada, preserva as características da MEC (LEONEL et al, 2016;BARRETO et al, 2017;KAKABADZE et al, 2019).…”

Section: Bioengenharia De Tecidosunclassified

“…O placentônio bovino, comparado com a placenta humana, tem tamanho razoável para enxertos, complexo sistema vascular com vasos grandes bem distribuídos e matriz extracelular preservada (BARRETO et al, 2017;KAKABADZE et al, 2019). Ainda produz com mais eficiência anastomoses após implantação, quando comparado à placenta humana (KAKABADZE; KAKABADZE, 2015).…”

Section: Bioengenharia De Tecidosunclassified

“…O placentônio bovino descelularizado foi empregado na bioengenharia de tecidos, como um "scaffold" biológico recelularizado com células hepáticas e translocado no fígado de ratos, com intuito de avaliar o arranjo vascular, manutenção de nutrientes, atuação metabólica e hemostase. Apresentou resultados expressivos que determinaram seu potencial uso na medicina regenerativa (KAKABADZE et al, 2019). Entretanto a sua estrutura e composição pós descelularização ainda não foi descrita…”

Section: Bioengenharia De Tecidosunclassified

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>b<Placenta bovina de clones descelularizada como fonte de scaffolds biológicos>/b<

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Bone Marrow Stem Cell and Decellularized Human Amniotic Membrane for the Treatment of Nonhealing Wound After Radiation Therapy

Cited by 24 publications

References 55 publications

Producing Human Amniotic Epithelial Cells-only Membrane for Transplantation

Producing Human Amniotic Epithelial Cells-only Membrane for Transplantation

Systematic Review of the Application of Perinatal Derivatives in Animal Models on Cutaneous Wound Healing

>b<Placenta bovina de clones descelularizada como fonte de scaffolds biológicos>/b<

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