Bone marrow stem cells in facial nerve regeneration from isolated stumps

Salomone, Raquel; Bento, Ricardo Ferreira; Costa, Heloisa Juliana Zabeu Rossi; Azzi-Nogueira, Deborah; Ovando, Patrícia C; Da-Silva, Ciro Ferreira; Zanatta, Daniela B; Haddad, Luciana A.

doi:10.1002/mus.23768

Cited by 28 publications

(39 citation statements)

References 53 publications

(146 reference statements)

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“…Therefore, the nerve can be injured by neuropraxia, axonotmosis or neurotmesis, the latter being more severe, which can lead to motor, sensory and functional losses, significantly interfering with the individual's daily, employment, social and emotional activities [1][2][3]. The incidence rate in the population compared to those of peripheral nerve damage is about 2% to 2.8%, reaching 5% when involving nerve plexuses and root nerves [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Low-Level Laser Therapy (LLLT) Improves the Repair Process of Peripheral Nerve Injuries: A Mini Review

Rosso¹,

Buchaim²,

Rosa³

et al. 2017

Int J Neurorehabilitation Eng

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Purpose: The aim of this study was to perform a mini-review on the effects of low-level laser therapy on nerve regeneration. Methods:In this study, the authors associated the new fibrin sealant derived from snake venom as a method of nerve repair end-to-side. Results and conclusion:The use of fibrin sealant effectively provided an axonal regeneration, and the association with low-level laser therapy elevated this regenerative process, as demonstrated in the morphometric and morphological analyzes.

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Section: Introductionmentioning

confidence: 99%

Low-Level Laser Therapy (LLLT) Improves the Repair Process of Peripheral Nerve Injuries: A Mini Review

Rosso¹,

Buchaim²,

Rosa³

et al. 2017

Int J Neurorehabilitation Eng

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“…A variety of experimental models have been used to study processes involving degeneration and regeneration of the peripheral nerves; animal models such as dogs and cats 1 , rabbits 2 , guinea pigs 3 , pigs 4 , and rats 5,6 have been used, each with its own advantages and disadvantages, but we still remain far from the ideal model.…”

Section: Introductionmentioning

confidence: 99%

Tibial and fibular nerves evaluation using intraoperative electromyography in rats

Nepomuceno

Politani²,

Silva³

et al. 2016

Acta Cir. Bras.

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Intellectual and scientific content of the study. ABSTRACT PURPOSE:To evaluate a new model of intraoperative electromyographic (EMG) assessment of the tibial and fibular nerves, and its respectives motor units in rats. METHODS:Eight Wistar rats underwent intraoperative EMG on both hind limbs at two different moments: week 0 and week 12.Supramaximal electrical stimulation applied on sciatic nerve, and compound muscle action potential recorded on the gastrocnemius muscle (GM) and the extensor digitorum longus muscle (EDLM) through electrodes at specifics points. Motor function assessment was performaced through Walking Track Test. RESULTS:Exposing the muscles and nerves for examination did not alter tibial (p=0.918) or fibular (p=0.877) function between the evaluation moments. Electromyography of the GM, innervated by the tibial nerve, revealed similar amplitude (p=0.069) and latency (p=0.256) at week 0 and at 12 weeks, creating a standard of normality. Meanwhile, electromyography of the EDLM, innervated by the fibular nerve, showed significant differences between the amplitudes (p=0.003) and latencies (p=0.021) at the two different moments of observation. CONCLUSION:Intraoperative electromyography determined and quantified gastrocnemius muscle motor unit integrity, innervated by tibial nerve. Although this study was not useful to, objectively, assess extensor digitorum longus muscle motor unit, innervated by fibular nerve.

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“…A técnica digital atual permite a contagem e medição das fibras mielinizadas a partir do software de análise de imagem (30,51).…”

Section: O Modelo Animalunclassified

“…O tamanho da amostra baseou-se em estudos anteriores que avaliaram a regeneração do nervo facial de ratos submetido a outros tratamentos (30,51,87 …”

Section: Analise Estatísticaunclassified

“…Ademais, a quantidade de axônios no interior desse ramo é relativamente constante e independe da idade e peso do animal (36,43). Outra vantagem reside na semelhança estrutural com o nervo facial de humanos, que também contém fascículos, ao contrário do tronco do NF dos ratos (36,42,51 (27,38,39,89). Além disso, várias publicações prévias atestam a influência do sexo dos animais sobre seu desempenho na regeneração neural.…”

Section: O Modelo Animalunclassified

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Sutura química por polietilenoglicol na regeneração do nervo facial em ratos após neurotmese

Nascimento¹

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, cujo apoio senti em cada passo desse trabalho, às vezes com risos altos, às vezes com broncas, e às vezes silenciosamente, proporcionando-me as condições de seguir adiante. Agradeço sobretudo por servir de inspiração para mim e toda uma geração de otorrinos, transformando a especialidade no Brasil e fora.Ao Prof. Dr. Rubens Vuono de Brito Neto, meu mentor e inspiração durante a residência e mesmo depois, no ombro de quem já obtive amparo inúmeras vezes.Ao Dr. Robinson Koji Tsuji, exemplo de profissional ético e competente, por todo conhecimento compartilhado e amizade.À Dra. Raquel Salomone, por quem minha admiração e amizade nunca param de crescer, agradeço imensamente os insights, o estímulo, a disponibilidade e a amizade que nunca vacila, nem mede esforços.À Dra. Heloisa Juliana Zabeu Rossi Costa, por me ajudar em todas as etapas desse estudo, desde a ideia original até a escrita da tese. À Dra. Anna Carolina de Oliveira Fonseca, minha amiga e confidente, que tantas vezes me acolheu e me tranquilizou, por ser um grande estímulo na conclusão desse trabalho.Aos amigos que fiz na residência e preceptoria de otorrinolaringologia, LISTA DE GRÁFICOS BACKROUND:The gold standard treatment for traumatic transection of the FN continues to be end-to-end anastomosis using fibrin glue, which often yields unsatisfying results. OBJECTIVE: To test the outcome of a novel method of polyethylene glycol (PEG)-fusion on FN transection using electrophysiological and histophormometric parameters. METHODS: Wistar rats were divided into 4 groups. After FN transection, the control group was submitted to end-to-end anastomosis with microsutures. Group 2 was submitted to microsutures plus the PEG-fusion protocol. This protocol consisted in bathing nerve stumps with a calcium-free Krebs solution containing methylene blue (MB) before suturing. After suturing, the repaired nerve received a PEG solution followed by a calciumcontaining Krebs solution. Group 3 received microsutures plus the MB solution and group 4 received microsutures plus the PEG solution. Compound muscle action potentials (CMAPs) were recorded before the intervention and 3 and 6 weeks afterwards. Histomorphometric analysis was done at 6 weeks time. RESULTS:The PEG-fusion protocol yielded larger CMAP amplitude, smaller CMAP duration at 3 and 6 weeks and a larger axon count and axon diameter. Between the other groups, no significant difference was seen. CONCLUSION: PEG-fusion produces better FN recovery after transection, when considering electrophysiological and histomorphometric analysis and may be of use in clinical scenarios of FN cut-severance followed by immediate repair.

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Bone marrow stem cells in facial nerve regeneration from isolated stumps

Abstract: Regeneration of the facial nerve was improved by both uBMSC and dBMSC in rats, yet uBMSC was associated with superior functional results.

Cited by 28 publications

References 53 publications

Low-Level Laser Therapy (LLLT) Improves the Repair Process of Peripheral Nerve Injuries: A Mini Review

Low-Level Laser Therapy (LLLT) Improves the Repair Process of Peripheral Nerve Injuries: A Mini Review

Tibial and fibular nerves evaluation using intraoperative electromyography in rats

Sutura química por polietilenoglicol na regeneração do nervo facial em ratos após neurotmese

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