Bone marrow stromal cells and resorbable collagen guidance tubes enhance sciatic nerve regeneration in mice

Lopes, Francisco R.; Campos, Lenira Camargo de Moura; Corrêa, José Dias; Balduino, Alex; Lora, S.; Langone, Francesco; Borojević, Radovan; Martínez, Ana Maria Blanco

doi:10.1016/j.expneurol.2005.12.019

Cited by 110 publications

(55 citation statements)

References 59 publications

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“…In this way, it is difficult to compare data obtained in the sciatic nerve with those achieved with one of its branches and to consider them as proximal and distal segments of the same nerve. The data on capillary area and density, referred for the first time in the present study, are important since angiogenesis could be a clue to explaining the regenerative effect of mononuclear bone marrow cells on sciatic nerve regeneration (Pereira-Lopes et al, 2006).…”

Section: Minimal Fascicular Area and Diameter And Capillariesmentioning

confidence: 99%

Aspects of the Macro and Microscopic Anatomy of the Sciatic Nerve in Wistar Rats

Suaid

Santos

Yamane³

et al. 2016

Int. J. Morphol.

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. Aspects of the macro and microscopic anatomy of the sciatic nerve in Wistar rats. Int. J. Morphol., 34(3):877-884, 2016. SUMMARY:The present study seeks to systematize morphological and morphometrical parameters and brings new data on the main branch of the lumbosacral plexus -i.e., sciatic nerve -in Wistar rats aged four and seven weeks. Sixteen female were divided into two groups, namely animals aged four weeks, and animals aged seven weeks. The specimens were studied at proximal and distal segments of the right hind limb sciatic nerves. Semi-thin transverse sections (0.25 µm thickness) were stained with 1 % toluidine blue, and the morphometric analysis was processed through the KS 400 software. Except for the number of fascicles and fascicular diameter, no differences were found between the proximal and distal segments. We observed differences when morphometric values were compared between 4-and 7-week old animals, with some exceptions (number of fascicles and myelinated fibers, and capillary area and number). The macroscopic data disagree with a previous description of the sciatic nerve being composed by two fascicles. Instead, sciatic nerve's only fascicle trifurcates or quadrifurcates at the distal third of the thigh. The total capillary area and density were calculated, and these are the first referential data for the sciatic nerve. Histograms of myelinated fiber and axons considering the animal ages were built. The results presented here are important because experimental studies, mainly studies on nerve regenerations require comparison with normal reliable data.

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Section: Minimal Fascicular Area and Diameter And Capillariesmentioning

confidence: 99%

Aspects of the Macro and Microscopic Anatomy of the Sciatic Nerve in Wistar Rats

Suaid

Santos

Yamane³

et al. 2016

Int. J. Morphol.

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“…First, it is difficult to have access to nerve donor to harvest sufficient Schwann cells [212,213]. Second, it is time-consuming to culture and expand Schwann cells in vitro, which may therefore delay treatment [214].…”

Section: Neural Stem Cellsmentioning

confidence: 99%

“…With regard to fiber area, myelin area and axon area, results from BMDC-filled tube were superior to those from the control group. In addition, the motor function recovery was also faster in the cell-seeded tube than the control tube [212]. Figure 6 shows the semi-thin cross-sections of regenerated nerve tissues of both cell-seeded (A and B) and control (C and D) groups.…”

Section: Mesenchymal Stem Cells (Mscs)mentioning

confidence: 99%

“…Figure 6(A) shows the regenerated nerve tissue, and Figure 6(B) shows both clusters of newly regenerated nerve fibers (arrows) and blood vessels. Figure 6(C) (control) shows a less organized regenerated nerve tissue with no pronounced clusters of newly regenerated nerve fibers, while only large and empty blood vessels (arrows) can be seen in D [212]. Di Summa et al [237] compared the effectiveness of Schwann cells, MSCs and adipose-derived stem cells (ADSCs) on enhancing peripheral neural regeneration by seeding each of them into a fibrin conduit to repair a 10-mm sciatic nerve gap in a rat model.…”

Section: Mesenchymal Stem Cells (Mscs)mentioning

confidence: 99%

“…Pereira Lopes et al [212] filled a biodegradable collagen tube with bone marrow-derived cells (BMDCs), a subset of MSCs, to bridge a 3-mm sciatic nerve gap in a mouse model. BMDCs were observed to express two neural growth factors, BDNF and NGFβ, the former of which was not expressed by the cultured cells.…”

Section: Mesenchymal Stem Cells (Mscs)mentioning

confidence: 99%

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Biodegradable Cell-Seeded Nanofiber Scaffolds for Neural Repair

Han

Cheung

2011

Polymers

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Central and peripheral neural injuries are traumatic and can lead to loss of motor and sensory function, chronic pain, and permanent disability. Strategies that bridge the site of injury and allow axonal regeneration promise to have a large impact on restoring quality of life for these patients. Engineered materials can be used to guide axonal growth. Specifically, nanofiber structures can mimic the natural extracellular matrix, and aligned nanofibers have been shown to direct neurite outgrowth and support axon regeneration. In addition, cell-seeded scaffolds can assist in the remyelination of the regenerating axons. The electrospinning process allows control over fiber diameter, alignment, porosity, and morphology. Biodegradable polymers have been electrospun and their use in tissue engineering has been demonstrated. This paper discusses aspects of electrospun biodegradable nanofibers for neural regeneration, how fiber alignment affects cell alignment, and how cell-seeded scaffolds can increase the effectiveness of such implants.

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