Bone marrow stromal cells interaction with titanium; Effects of composition and surface modification

Duvvuru, Murali Krishna; Han, Weiguo; Chowdhury, Prantik Roy; Vahabzadeh, Sahar; Sciammarella, Federico M.; Elsawa, Sherine F.

doi:10.1371/journal.pone.0216087

Cited by 16 publications

(10 citation statements)

References 35 publications

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“…Studies to analyze the effect of different titanium implant surface modifications on in vitro osteogenesis were conducted using various mammalian cell lines, such as MSCs or murine calvarial (pre)osteoblasts. 48,[61][62][63][64][65][66][67][68] As summarized in Table 2, the majority of the reviewed studies used disc 69,48,[61][62][63][64]70,71,65 or rectangular 66,72,67 shaped titanium implants with varying surface modifications, for example, gritblasting, magnetron sputtering or acidic treatment.…”

Section: Implant Surface Modification Techniquesmentioning

confidence: 99%

Implant‐bone‐interface: Reviewing the impact of titanium surface modifications on osteogenic processes in vitro and in vivo

Stich

Alagboso

Křenek

et al. 2021

Bioengineering & Transla Med

101

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Titanium is commonly and successfully used in dental and orthopedic implants. However, patients still have to face the risk of implant failure due to various reasons, such as implant loosening or infection. The risk of implant loosening can be countered by optimizing the osteointegration capacity of implant materials. Implant surface modifications for structuring, roughening and biological activation in favor for osteogenic differentiation have been vastly studied. A key factor for a successful stable longterm integration is the initial cellular response to the implant material. Hence, cellmaterial interactions, which are dependent on the surface parameters, need to be considered in the implant design. Therefore, this review starts with an introduction to the basics of cell-material interactions as well as common surface modification techniques. Afterwards, recent research on the impact of osteogenic processes in vitro and vivo provoked by various surface modifications is reviewed and discussed, in order to give an update on currently applied and developing implant modification techniques for enhancing osteointegration.

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Section: Implant Surface Modification Techniquesmentioning

confidence: 99%

Implant‐bone‐interface: Reviewing the impact of titanium surface modifications on osteogenic processes in vitro and in vivo

Stich

Alagboso

Křenek

et al. 2021

Bioengineering & Transla Med

101

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“…In vivo, CCL2 is one of the main chemokines induced in osteoblasts in response to bacterial infections (Galliera et al, 2008). Pro-inflammatory cytokine IL-6 (Duvvuru et al, 2019) expression decreased in hASCs grown on the Bio-Oss ® / Avitene scaffold under analysis. (Mahon et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Stem Cell Fate and Immunomodulation Promote Bone Regeneration via Composite Bio-Oss®/AviteneTM Biomaterial

Iaquinta

Martini

D’Agostino

et al. 2022

Front. Bioeng. Biotechnol.

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Bone defects in maxillofacial regions lead to noticeable deformity and dysfunctions. Therefore, the use of biomaterials/scaffolds for maxillofacial bone regrowth has been attracting great interest from many surgical specialties and experts. Many approaches have been devised in order to create an optimal bone scaffold capable of achieving desirable degrees of bone integration and osteogenesis. Osteogenesis represents a complex physiological process involving multiple cooperating systems. A tight relationship between the immune and skeletal systems has lately been established using the concept of “osteoimmunology,” since various molecules, particularly those regulating immunological and inflammatory processes, are shared. Inflammatory mediators are now being implicated in bone remodeling, according to new scientific data. In this study, a profiler PCR array was employed to evaluate the expression of cytokines and chemokines in human adipose derived-mesenchymal stem cells (hASCs) cultured on porous hydroxylapatite (HA)/Collagen derived Bio-Oss®/Avitene scaffolds, up to day 21. In hASCs grown on the Bio-Oss®/Avitene biomaterial, 12 differentially expressed genes (DEGs) were found to be up-regulated, together with 12 DEG down-regulated. Chemokine CCL2, which affects bone metabolism, tested down-regulated. Interestingly, the Bio-Oss®/Avitene induced the down-regulation of pro-inflammatory inter-leukin IL-6. In conclusion, our investigation carried out on the Bio-Oss®/Avitene scaffold indicates that it could be successfully employed in maxillofacial surgery. Indeed, this composite material has the advantage of being customized on the basis of the individual patients favoring a novel personalized medicine approach.

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“…The main goal of recent researches is to identify a material with a high performance, able to guarantee direct contact between bone and implant, and to absorb biological molecules on its surface [38]. Concerning titanium, a great number of methods have been introduced to modify implants surface, such as physicochemical, morphological, and biochemical techniques [39]. Acid etching and sandblasting have been reported to produce high-performing surfaces [40].…”

Section: Discussionmentioning

confidence: 99%

VEGF/VEGF-R/RUNX2 Upregulation in Human Periodontal Ligament Stem Cells Seeded on Dual Acid Etched Titanium Disk

et al. 2020

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In restorative dentistry, the main implants characteristic is the ability to promote the osseointegration process as the result of interaction between angiogenesis and osteogenesis events. On the other hand, implants cytocompatibility remains a necessary feature for the success of surgery. The purpose of the current study was to investigate the interaction between human periodontal stem cells and two different types of titanium surfaces, to verify their cytocompatibility and cell adhesion ability, and to detect osteogenic and angiogenic markers, trough cell viability assay (MTT), Confocal Laser Scanning Microscopy (CLSM), scanning electron microscopy (SEM), and gene expression (RT-PCR). The titanium surfaces, machined (CTRL) and dual acid etched (TEST), tested in culture with human periodontal ligament stem cells (hPDLSCs), were previously treated in two different ways, in order to evaluate the effects of CTRL and TEST and define the best implant surface. Furthermore, the average surface roughness (Ra) of both titanium surfaces, CTRL and TEST, has been assessed through atomic force microscopy (AFM). The vascular endothelial growth factor (VEGF) and Runt-related transcription factor 2 (RUNX2) expressions have been analyzed by RT-PCR, WB analysis, and confocal laser scanning microscopy. Data evidenced that the different morphology and topography of the TEST disk increased cell growth, cell adhesion, improved osteogenic and angiogenic events, as well osseointegration process. For this reason, the TEST surface was more biocompatible than the CTRL disk surface.

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Bone marrow stromal cells interaction with titanium; Effects of composition and surface modification

Cited by 16 publications

References 35 publications

Implant‐bone‐interface: Reviewing the impact of titanium surface modifications on osteogenic processes in vitro and in vivo

Implant‐bone‐interface: Reviewing the impact of titanium surface modifications on osteogenic processes in vitro and in vivo

Stem Cell Fate and Immunomodulation Promote Bone Regeneration via Composite Bio-Oss®/AviteneTM Biomaterial

VEGF/VEGF-R/RUNX2 Upregulation in Human Periodontal Ligament Stem Cells Seeded on Dual Acid Etched Titanium Disk

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