1992
DOI: 10.1016/0169-6009(92)90807-p
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Bone marrow stromal colony formation requires stimulation by haemopoietic cells

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Cited by 126 publications
(92 citation statements)
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“…In the bone marrow niche, MSCs and hematopoietic cells dwell as neighbors that support each other through secretion of various growth factors, cytokines, and chemokines [65,83]. Cocultured hematopoietic cells may regulate the phenotype of MSCs, possibly by maintaining a higher fraction of clonogenic MSCs [56]. After 3-week perfusion expansion of a fresh bone marrow aspirate, hematopoietic cells identified as CD45 + (common leukocyte antigen) were retained on a hydroxyapatite scaffold.…”
Section: D Expansionmentioning
confidence: 98%
See 1 more Smart Citation
“…In the bone marrow niche, MSCs and hematopoietic cells dwell as neighbors that support each other through secretion of various growth factors, cytokines, and chemokines [65,83]. Cocultured hematopoietic cells may regulate the phenotype of MSCs, possibly by maintaining a higher fraction of clonogenic MSCs [56]. After 3-week perfusion expansion of a fresh bone marrow aspirate, hematopoietic cells identified as CD45 + (common leukocyte antigen) were retained on a hydroxyapatite scaffold.…”
Section: D Expansionmentioning
confidence: 98%
“…The observed decrease in CFE following the density gradient isolations cannot be attributed to a reduced number of plated cells from purification losses because the trend describes a subsequent passage wherein the plated cell number was normalized. In contrast to density gradient isolation, direct plating may enable the initial retention of hematopoietic cells, which secrete signals that maintain a higher fraction of clonogenic MSCs [56]. CFE decreases with increasing passage [17,55,57], and especially at higher passages, CFE accurately correlates with remaining cumulative population doublings [57].…”
Section: Colony-forming Efficiencymentioning
confidence: 99%
“…(16) Furthermore, TSP2-null MSCs, when cultured in vitro, show increased DNA synthesis as determined by tritiated thymidine incorporation. (16) Proliferation of MSCs in vitro is sensitive to a variety of extracellular molecules including systemic hormones such as vitamin D (17) and dexamethasone (18) and growth factors (19) such as basic FGF (bFGF), (20) platelet-derived growth factor (PDGF), (21) and the bone morphogenetic protein secreted protein (BMP) superfamily members. (22,23) The effect of extracellular matrix (ECM) proteins on MSC proliferation has not been studied as extensively.…”
Section: Introductionmentioning
confidence: 99%
“…Another possibility is the inherent selectivity of adherent rapidly proliferating cells in standard monolayer cultures. It is well known in other tissues such as bone marrow that non-adherent cells are necessary for the correct function of the mesenchymal-derived cells Friedenstein et al 1992;Rickard et al 1995). By inducing the cells to adhere by cytocentrifugation this possible culture artefact can be avoided and the behaviour of either adherent cells or adherent plus non-adherent cells observed.…”
Section: Discussionmentioning
confidence: 99%