Bone morphogenetic protein 4 induces efficient hematopoietic differentiation of rhesus monkey embryonic stem cells in vitro

Li, Fēi; Lu, Shi‐Jiang; Vida, Loyda N.; Thomson, James A.; Honig, George R.

doi:10.1182/blood.v98.2.335

Cited by 110 publications

(95 citation statements)

References 35 publications

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“…Inasmuch as many of the genes we undertook to study have not been cloned from the rhesus monkey, whenever rhesus monkey-specific sequences were unavailable, we employed the sequences of the corresponding human genes to design our PCR primers, assuming that the generally close homology between human and rhesus monkey would compensate for minor differences in gene sequences in PCR amplification [25]; this approach has been applied successfully for a large number of other genes in our previous study and other studies [7,11,25,26]. The sense and antisense primer sequences and the corresponding cDNA PCR product sizes are shown in Table 1 …”

Section: Gene Expression Quantification By Semiquantitative Pcrmentioning

confidence: 99%

“…Rhesus monkey ES cells were grown under differentiation conditions as described [7], then were replated and cultured for erythrocyte formation in vitro. After …”

Section: Enrichment Of Rhesus Monkey Erythroblasts From Es Cell Culturesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Under appropriate culture conditions, ES cells undergo differentiation in vitro to form hematopoietic precursors [5][6][7][8]. Flow cytometry analyses of rhesus monkey ES-cell-derived hematopoietic progenitor cells demonstrated that a substantial percentage of these cells expressed CD34 antigen and had morphological features of undifferentiated blast cells [7]. The hematopoietic character of these precursors is further supported by the demonstration that they express genes associated with early hematopoietic differentiation, and that they exhibit morphological findings of erythroid and myeloid lineages among their progeny cells [7].…”

Section: Introductionmentioning

confidence: 99%

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Hematopoietic Progenitor Cells Derived from Embryonic Stem Cells: Analysis of Gene Expression

Lu¹,

Quan²,

Li³

et al. 2002

STEM CELLS

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Rhesus monkey embryonic stem (ES) cells undergo differentiation in vitro towere obtained with the latter system, suggesting that this variation may be species specific. We observed, however, that CD34 + and CD34 + CD38 -cells derived from ES cells expressed embryonic ε and ζ as well as α, β, and γ globin genes, whereas no expression of embryonic globins could be detected in the cell preparations from BM. Moreover, erythroblast-enriched CD34 -cells derived from 4-or 5-week ES cell differentiation cultures also expressed embryonic, fetal, and adult globin genes, with greater β gene expression, but otherwise were identical to those of the more primitive CD34 + cells derived from 2-week ES cultures. These latter observations may reflect the presence of heterogeneous cell populations within the cell fractions that were compared, or they may represent variability among ES-cell-derived hematopoietic stem cells.

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Section: Gene Expression Quantification By Semiquantitative Pcrmentioning

confidence: 99%

“…Rhesus monkey ES cells were grown under differentiation conditions as described [7], then were replated and cultured for erythrocyte formation in vitro. After …”

Section: Enrichment Of Rhesus Monkey Erythroblasts From Es Cell Culturesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Hematopoietic Progenitor Cells Derived from Embryonic Stem Cells: Analysis of Gene Expression

Lu¹,

Quan²,

Li³

et al. 2002

STEM CELLS

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“…Medium to support the differentiation was described elsewhere. 29 Cells at day 18 were placed in Methocult GF+ media (StemCell Technologies, Vancouver, Canada) at 1 Â 10 4 and 1 Â 10 5 cells per plate and clonogenic hematopoietic colonies were produced. After 14 days, individual colonies were removed and spun onto glass slides.…”

Section: Hematopoietic Differentiationmentioning

confidence: 99%

Efficient and stable Sendai virus-mediated gene transfer into primate embryonic stem cells with pluripotency preserved

et al. 2004

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Efficient gene transfer and regulated transgene expression in primate embryonic stem (ES) cells are highly desirable for future applications of the cells. In the present study, we have examined using the nonintegrating Sendai virus (SeV) vector to introduce the green fluorescent protein (GFP) gene into non-human primate cynomolgus ES cells. The GFP gene was vigorously and stably expressed in the cynomolgus ES cells for a year. The cells were able to form fluorescent teratomas when transplanted into immunodeficient mice. They were also able to differentiate into fluorescent embryoid bodies, neurons, and mature blood cells. In addition, the GFP expression levels were reduced dose-dependently by the addition of an anti-RNA virus drug, ribavirin, to the culture. Thus, SeV vector will be a useful tool for efficient gene transfer into primate ES cells and the method of using antiviral drugs should allow further investigation for regulated SeV-mediated gene expression.

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Germ Layer Induction in ESC—Following the Vertebrate Roadmap

Smith

Wardle

Loose

et al. 2007

CP Stem Cell Biology

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Controlled differentiation of pluripotential cells takes place routinely and with great success in developing vertebrate embryos. It therefore makes sense to take note of how this is achieved and use this knowledge to control the differentiation of embryonic stem cells (ESCs). An added advantage is that the differentiated cells resulting from this process in embryos have proven functionality and longevity. This unit reviews what is known about the embryonic signals that drive differentiation in one of the most informative of the vertebrate animal models of development, the amphibian Xenopus laevis. It summarizes their identities and the extent to which their activities are dose‐dependent. The unit details what is known about the transcription factor responses to these signals, describing the networks of interactions that they generate. It then discusses the target genes of these transcription factors, the effectors of the differentiated state. Finally, how these same developmental programs operate during germ layer formation in the context of ESC differentiation is summarized. Curr. Protoc. Stem Cell Biol. 1:1D.1.1‐1D.1.22. © 2007 by John Wiley & Sons, Inc.

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Bone morphogenetic protein 4 induces efficient hematopoietic differentiation of rhesus monkey embryonic stem cells in vitro

Cited by 110 publications

References 35 publications

Hematopoietic Progenitor Cells Derived from Embryonic Stem Cells: Analysis of Gene Expression

Hematopoietic Progenitor Cells Derived from Embryonic Stem Cells: Analysis of Gene Expression

Efficient and stable Sendai virus-mediated gene transfer into primate embryonic stem cells with pluripotency preserved

Germ Layer Induction in ESC—Following the Vertebrate Roadmap

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