Bone morphogenetic protein-7 expression is down-regulated in human clear cell renal carcinoma

Bašić‐Jukić, Nikolina; Hudolin, Tvrtko; Radic-Antolic, Margareta; Čorić, Marijana; Zadro, Renata; Kaštelan, Željko; Pasini, Josip; Bandić-Pavlović, Daniela; Kes, Petar

doi:10.5301/jn.2010.2020

Cited by 9 publications

(12 citation statements)

References 31 publications

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“…We found higher expression of BMP7 in the RCCs than in the normal tissue, and BMP7 expression was lost in CC-RCCs with higher tumor stage. Thus, our results are partly in concordance with the recent study which reported BMP7 mRNA expression and protein were downregulated in CC-RCC [18]. Kwak et al [14] have reported that the BMP7 protein expression rate in NCC-RCC was higher than that in the CC-RCC.…”

Section: Discussionsupporting

confidence: 83%

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mRNA Expression of Bone Morphogenetic Proteins and Their Receptors in Human Renal Cell Carcinoma

Markić

Ćelić²,

Gršković

et al. 2011

Urol Int

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Introduction: Bone morphogenetic proteins (BMPs) have been studied in several cancers, but only limited information is available about renal cell carcinomas (RCCs). We determined the expression of mRNA of several BMP ligands and BMP receptors (BMPRs) in healthy kidney tissue and RCCs, and data were compared to clinicopathological parameters. Material and Methods: Sixty-four samples of RCCs and healthy renal tissues were prospectively examined. The expression of BMP2, BMP4, BMP6, BMP7, BMPRIA, BMPRIB and BMPRII mRNA was determined using semiquantitative reverse transcriptase-polymerase chain reaction. Results: The expression levels of different BMP ligands and BMPRs were considerably higher in RCCs than in normal kidney tissue. BMP ligands showed elevated expression in clear-cell RCCs, whereas all three BMPRs showed higher expression levels in non-clear-cell RCCs. In clear-cell RCCs, the expression levels of BMP2 progressively increased and expression levels of BMP6, BMP7 and BMPRIB were lost with higher tumor stage. Conclusions: All BMPs and their receptors have stronger expression levels in RCC. The expression level of BMP2 is strongly elevated in kidney cancer.

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Section: Discussionsupporting

confidence: 83%

“…It has also been found that the BMP signaling pathway is involved in tumorigenesis and can be used as molecular targets for cancer therapy. BMPs have hardly been investigated in human renal cell carcinomas (RCCs) and most of the published studies have explored BMP4, BMP6 and BMP7 [14][15][16][17][18].…”

mentioning

confidence: 99%

mRNA Expression of Bone Morphogenetic Proteins and Their Receptors in Human Renal Cell Carcinoma

Markić

Ćelić²,

Gršković

et al. 2011

Urol Int

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“…In keeping with this, data from the MTT cell proliferation assay showed no differences in cell viability and proliferation between control and A549 cells. BMP7 has been indicated to act as a mediator/regulator of cell survival and apoptosis in several tumour cell lines (3,(6)(7)(8)13,25,26,27), including prostate cancer cells, colon cancer cells, breast cancer cells and melanoma cells and renal cancer cells. In the present study, our results from the apoptosis assay showed that BMP7 offers no significant protection against apoptosis in the tumour cells transfected with BMP7 in vitro.…”

Section: Discussionmentioning

confidence: 99%

Μolecular impact of bone morphogenetic proteinï¿½7, on lung cancer cells and its clinical significance

et al. 2012

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Abstract. The aim of this study was to investigate the expression of bone morphogenetic protein 7 (BMP7), in human pulmonary cancer tissues/cells and to evaluate the cellular impact of bone morphogenetic proteins on pulmonary cancer cells. BMP7 expression was determined in human lung cancer cell lines. The invasiveness and growth of cells transfected with BMP7, in vitro, were evaluated using the in vitro invasion assay and in vitro tumour models. Cellular migration was analysed using wounding assays. BMP7-positive tumours correlated with the absence of bone metastasis (P=0.040). In this analysis, we identified that 4 of 4 small cell lung cancer (SCLC) tissue specimens had no BMP7 expression, which illustrated that BMP7 may have no role in SCLC. BMP7 expression was not correlated with the overall survival time in lung cancer patients. Downregulation of BMP7 expression significantly inhibited the invasiveness of SPC-A1 cells (P<0.001) and forced-expression of BMP7 dramatically increased the motility of A549 cells. Overexpression of BMP7 in A549 cells and its knockdown in SPC-A1 cells did not significantly alter proliferation compared with the control cells (P>0.5 respectively). In conclusion, we have demonstrated that BMP7 has an important role in controlling lung cancer cell motility and invasiveness, without affecting the growth process, cell proliferation and cell apoptosis. A higher BMP7 expression may be an indicator for bone metastasis. The therapeutic role of BMP7 warrants further investigation. IntroductionLung cancer is one of the most fatal tumours worldwide. Distant metastasis is an important cause for the poor prognosis. Of the factors related to metastasis, bone morphogenetic proteins (BMPs) have been recently shown to regulate the aggressiveness of cancer cells (1-7). BMPs are multifunctional signaling molecules belonging to the transforming growth factor-β (TGF-β) superfamily, which play important roles in multiple cellular processes such as cell growth, differentiation, migration and apoptosis in various types of cancer (8-12). The Smad-dependent signaling pathway has been proven to be the canonical pathway for BMP function (13). In lung cancer, expression of the BMP family members has also been reported (1,14). For example, BMP2 protein could stimulate growth, migration and invasion of lung cancer cells, and is overexpressed in virtually all types of lung cancers, including non-small cell lung cancer (NSCLC) and small cell lung cancer (SCLC). Silencing of BMP3b expression during NSCLC development has been reported and the BMP3b promoter has been found to be methylated. Activation of the BMP4 signaling pathway negatively regulates the growth, and induces senescence of A549 lung adenocarcinoma cells. Thus, there may be a contrasting response to any given BMP, depending on the tumour and cell type. Despite the discordant results defining the specific effects of BMP7 in various types of cancer, published data underscore the important role that BMP7 plays in tumour development and metastasis. The expre...

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“…The expression of TGF-β/BMP proteins is most commonly regulated at the mRNA level. As an example, this is particularly evident in BMP-7 where changes in Bmp7 mRNA expression occur following renal injury (Simon et al, 1999) and during tumorigenesis (Basic-Jukic et al, 2011). While the mechanisms regulating Bmp7 transcription are poorly understood, the Bmp7 promoter has been cloned and shown to contain numerous putative transcription factor binding sites (Dorai et al, 2001;Simon, Feliers, Arar, Bhandari, & Abboud, 2002).…”

Section: Ligand Expressionmentioning

confidence: 97%

BMP-7 Signaling and its Critical Roles in Kidney Development, the Responses to Renal Injury, and Chronic Kidney Disease

Manson

Austin

Guo

et al. 2015

Bone Morphogenic Protein

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Bone morphogenetic protein-7 expression is down-regulated in human clear cell renal carcinoma

Cited by 9 publications

References 31 publications

mRNA Expression of Bone Morphogenetic Proteins and Their Receptors in Human Renal Cell Carcinoma

mRNA Expression of Bone Morphogenetic Proteins and Their Receptors in Human Renal Cell Carcinoma

Μolecular impact of bone morphogenetic proteinï¿½7, on lung cancer cells and its clinical significance

BMP-7 Signaling and its Critical Roles in Kidney Development, the Responses to Renal Injury, and Chronic Kidney Disease

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