Bone morphogenic protein-2 immobilization by cold atmospheric plasma to enhance the osteoinductivity of carboxymethyl chitosan-based nanofibers

Sharifi, Fereshteh; Atyabi, Seyed Mohammad; Irani, Shiva; Bakhshi, Hadi

doi:10.1016/j.carbpol.2019.115681

Cited by 33 publications

(24 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The viability of cells on all scaffolds was progressive during this time and depended on the CMC/LAP content (Figure 3A). Cell viability was significantly higher for the PCL@CMC/LAP3.5% scaffold compared to other scaffolds and control (tissue culture plate) during these days ( P ≤ .001), which can be due to its higher surface hydrophilicity and enriched concentrations of CMC, LAP nanoplatelets and its dissolution products (Li + , Na + , Mg 2+ , and Si[OH] 4 ) 22,23 . Therefore, the PCL@CMC/LAP3.5% scaffold was selected as the scaffold with optimal CMC/LAP content for the next investigations.…”

Section: Resultsmentioning

confidence: 99%

“…To study the osteodifferentiation of hBM‐MSCs seeded on the scaffolds, the expressions of ALP and Osteonectin in mRNA levels were quantitatively evaluated through real‐time PCR after 21 days. ALP is an early osteodifferentiation marker for immature osteoblast 23 . Osteonectin is highly expressed at the beginning of the osteodifferentiation, but its expression decreases as the cells acquire characteristics of mature osteoblasts 33 .…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Immobilization of carboxymethyl chitosan/laponite on polycaprolactone nanofibers as osteoinductive bone scaffolds

Arab‐Ahmadi

Irani

Bakhshi

et al. 2020

Polymers for Advanced Techs

Self Cite

View full text Add to dashboard Cite

The side effects and high cost of growth factors and drugs in bone tissue engineering have led to outstanding investigations of alternative osteoinductive supplements. Herein, the electrospun polycaprolactone (PCL) nanofibers were immobilized with carboxymethyl chitosan (CMC) and laponite nanoplatelets (LAP, 0.5‐3.5 wt%) to fabricate osteoinductive scaffolds for bone tissue engineering. The Fourier‐transform infrared)FTIR(and energy dispersive X‐ray (EDX) spectroscopy confirmed the chemical immobilization of CMC and LAP on the surface of PCL nanofibers. Water contact angle measurements exhibited significant improvement of surface hydrophilicity for immobilized scaffolds (<10°) comparing to the virgin PCL nanofibers (125°). The immobilization of CMC and LAP enhanced the attachment, proliferation, and osteodifferentiation of the seeded human bone marrow mesenchymal stem cells (hBM‐MSCs), as evidenced by increased calcium deposition, alkaline phosphatase (ALP) activity, and Osteonectin gene expression. Therefore, the fabricated scaffolds can serve as an appropriate substrate to support the proliferation and differentiation of stem cells to osteoplasts without any external osteoinductive stimulation.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Immobilization of carboxymethyl chitosan/laponite on polycaprolactone nanofibers as osteoinductive bone scaffolds

Arab‐Ahmadi

Irani

Bakhshi

et al. 2020

Polymers for Advanced Techs

Self Cite

View full text Add to dashboard Cite

show abstract

“…In this regard, HA is one of the most common ceramics which is used for this application [82]. Likewise, in numerous researches CS is incorporated into the synthetic-based polymer, including PCL, PLLA [85][86][87][88][89][90][91], or synthetic polymers and ceramics are incorporated at the same time [92][93][94][95]. Even though synthetic polymers offer positive characteristics including cytocompatibility and sufficient mechanical performance, their hydrophobicity and the absence of particular cell recognition sites restrict their functional application.…”

Section: Bone Regenerationmentioning

confidence: 99%

Three-Dimensional Printing Constructs Based on the Chitosan for Tissue Regeneration: State of the Art, Developing Directions and Prospect Trends

et al. 2020

View full text Add to dashboard Cite

Chitosan (CS) has gained particular attention in biomedical applications due to its biocompatibility, antibacterial feature, and biodegradability. Hence, many studies have focused on the manufacturing of CS films, scaffolds, particulate, and inks via different production methods. Nowadays, with the possibility of the precise adjustment of porosity size and shape, fiber size, suitable interconnectivity of pores, and creation of patient-specific constructs, 3D printing has overcome the limitations of many traditional manufacturing methods. Therefore, the fabrication of 3D printed CS scaffolds can lead to promising advances in tissue engineering and regenerative medicine. A review of additive manufacturing types, CS-based printed constructs, their usages as biomaterials, advantages, and drawbacks can open doors to optimize CS-based constructions for biomedical applications. The latest technological issues and upcoming capabilities of 3D printing with CS-based biopolymers for different applications are also discussed. This review article will act as a roadmap aiming to investigate chitosan as a new feedstock concerning various 3D printing approaches which may be employed in biomedical fields. In fact, the combination of 3D printing and CS-based biopolymers is extremely appealing particularly with regard to certain clinical purposes. Complications of 3D printing coupled with the challenges associated with materials should be recognized to help make this method feasible for wider clinical requirements. This strategy is currently gaining substantial attention in terms of several industrial biomedical products. In this review, the key 3D printing approaches along with revealing historical background are initially presented, and ultimately, the applications of different 3D printing techniques for fabricating chitosan constructs will be discussed. The recognition of essential complications and technical problems related to numerous 3D printing techniques and CS-based biopolymer choices according to clinical requirements is crucial. A comprehensive investigation will be required to encounter those challenges and to completely understand the possibilities of 3D printing in the foreseeable future.

show abstract

“…In addition, nanofibers are highly porous, providing an artificial milieu that is structurally comparable to the naturally occurring extracellular matrix (ECM) ( 1 , 5 ). As such, electrospun nanofibers are frequently utilized in tissue engineering ( 1 , 6 , 7 ). Electrospinning can also produce nanofibers with high bioactivity.…”

Section: Introductionmentioning

confidence: 99%

Features of a simvastatin‑loaded multi‑layered co‑electrospun barrier membrane for guided bone regeneration

Huang

Chu³

et al. 2021

Exp Ther Med

View full text Add to dashboard Cite

A novel tri-layer membrane consisting of polycaprolactone (PCL) fibrous sheets and structured nanofibers with a gelatin (Gt) shell and a simvastatin-containing PCL core (PCL-Gt/PCL-simvastatin membrane) was prepared. The soft external layer comprised of Gt/PCL-simvastatin, the external layer of PCL and the middle layer of both microfilaments, interwoven together. The membrane was designed to promote osteoinduction and act as a barrier against cells but not against water and molecules in order to promote guided bone regeneration. The structure of the membrane was characterized by scanning electronic microscopy. The in vitro release rates of simvastatin over 32 days were determined by high-performance liquid chromatography. For in vitro biological assays, bone marrow mesenchymal stem cells and human fibroblasts were cultured on the different surfaces of the membrane. Cell adhesion, proliferation, distribution, and differentiation were examined. For in vivo testing, cranial defects were created in rabbits to assess the amount of new bone formed for each membrane. The results revealed that membranes with multi-layered structures showed good cell viability and effective osteoinductive and barrier properties. These results suggest that the novel multi-layered PCL-Gt/PCL-simvastatin membranes have great potential for bone tissue engineering.

show abstract

Bone morphogenic protein-2 immobilization by cold atmospheric plasma to enhance the osteoinductivity of carboxymethyl chitosan-based nanofibers

Cited by 33 publications

References 44 publications

Immobilization of carboxymethyl chitosan/laponite on polycaprolactone nanofibers as osteoinductive bone scaffolds

Immobilization of carboxymethyl chitosan/laponite on polycaprolactone nanofibers as osteoinductive bone scaffolds

Three-Dimensional Printing Constructs Based on the Chitosan for Tissue Regeneration: State of the Art, Developing Directions and Prospect Trends

Features of a simvastatin‑loaded multi‑layered co‑electrospun barrier membrane for guided bone regeneration

Contact Info

Product

Resources

About