Bone regeneration by statin and low-intensity pulsed ultrasound (LIPUS) in rabbit nasal bone

Ishihara, Yuri; Ueki, Koichiro; Sotobori, Megumi; Marukawa, Kohei; Moroi, Akinori

doi:10.1016/j.jcms.2013.05.002

Cited by 22 publications

(20 citation statements)

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“…Therefore, a valid treatment is imperative for preventing collapse of the femoral head and subsequent osteoarthritis. Several studies have reported that LIPUS can enhance bone repair and local blood flow in animal models [3,16,24,34]. However, whether the effect of osteogenesis and neovascularization by LIPUS can enhance the repair progress in steroid-associated osteonecrosis is unknown.…”

Section: Discussionmentioning

confidence: 99%

“…In vitro studies have shown that LIPUS increases bone formation, osteoblast activity, and osteogenic differentiation of mesenchymal stem cells (MSCs) [27,31]. In vivo studies have found that LIPUS treatment promotes new bone formation and improves tissue blood flow [3,16,24,34]. Rutten et al [29], in a clinical study, showed that LIPUS accelerates fracture healing through increased osteoblast activity.…”

Section: Introductionmentioning

confidence: 99%

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Low-intensity Pulsed Ultrasound Enhances Bone Repair in a Rabbit Model of Steroid-associated Osteonecrosis

Zhu

Cai

Lin

et al. 2015

Clinical Orthopaedics &Amp; Related Research

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Background Steroids are a leading cause of femoral head osteonecrosis. Currently there are no medications available to prevent and/or treat steroid-associated osteonecrosis. Low-intensity pulsed ultrasound (LIPUS) was approved by the FDA for treating delayed union of bone fractures. Some studies have reported that LIPUS can enhance bone formation and local blood flow in an animal model of fracture healing. However, whether the effect of osteogenesis and neovascularization by LIPUS can enhance the repair progress in steroid-associated osteonecrosis is unknown.Questions/purposes We hypothesized that LIPUS may facilitate osteogenesis and neovascularization in the reparative processes of steroid-associated osteonecrosis. Using a rabbit animal model, we asked whether LIPUS affects (1) bone strength and trabecular architecture; (2) blood vessel number and diameter; and (3) BMP-2 and VEGF expression. Methods Bilateral femoral head necrosis was induced by lipopolysaccharide and methylprednisolone in 24 rabbits. The left femoral heads of rabbits received LIPUS therapy (200 mW/cm 2 ) for 20 minutes daily and were classified as the LIPUS group. The right femoral heads of the same rabbits did not receive therapy and were classified as the control group. All rabbits were euthanized 12 weeks after LIPUS therapy. Micro-CT, biomechanical testing, histologic evaluation, immunohistochemistry, quantitative realtime PCR, and Western blot were used for examination of the effects of LIPUS. Results Twelve weeks after LIPUS treatment, the loading strength in the control group was 355 ± 38 N (95% CI, 315-394 N), which was lower (p = 0.028) than that in the LIPUS group (441 ± 78 N; 95% CI, 359-524 N). The bone tissue volume density (bone volume/total volume) in the LIPUS group (49.29% ± 12.37%; 95 % CI, 36.31%-62.27%) was higher (p = 0.022) than that in the control group (37.93% ± 8.37%; 95 % CI, 29.15%-46.72%). The percentage of empty osteocyte lacunae in the LIPUS group (17% ± 4%; 95% CI, 15%-20%) was lower (p = 0.002) than that in the control group (26% ± 9%; 95% CI, 21%-32%). The mineral apposition rate (lm/day) in the LIPUS group (2.3 ± 0.8 lm/day; 95% CI, 1.8 2.8 lm/day) was higher (p = 0.001) than that in the control group (1.6 ± 0.3 lm/day; 95% CL, 1.4-1.8 lm/day). The number of blood vessels in the LIPUS group (7.8 ± 3.6/mm 2 ; 95% CI, 5.5-10.1 mm 2 ) was greater (p = 0.025) than the number in Hanxiao Zhu and Xunzi Cai contributed equally to this work and should be considered as co-first authors.The institution of the authors has received funding from the National Natural Science Foundation of China (Hangzhou, Zhejiang, China) (81101377, 81101345, 81171687, 81371954, 81201414, 81201416, 81401785, 81472113) 123Clin Orthop Relat Res (2015) 473:1830-1839 DOI 10.1007 Clinical Orthopaedics and Related Research ® A Publication of The Association of Bone and Joint Surgeons® the control group (5.7 ± 2.6/mm 2 ; 95% CI, 4.0-7.3 mm 2 ). Messenger RNA (mRNA) and protein expression of BMP-2 in the LIPUS group (75 ± 7, 95% CI, 70-7...

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Low-intensity Pulsed Ultrasound Enhances Bone Repair in a Rabbit Model of Steroid-associated Osteonecrosis

Zhu

Cai

Lin

et al. 2015

Clinical Orthopaedics &Amp; Related Research

View full text Add to dashboard Cite

show abstract

“…Some studies have proposed that ultrasound treatment enhances the repair of fractured bone (Hasuike et al 2011;Ishihara et al 2014;Lim et al 2013;Yang and Park 2001); however, the energy from non-targeted exposure to ultrasound can diffuse, resulting in a decrease in positive effects. Therefore, in this study we investigated focused ultrasound (FUS), which can deliver highly focused acoustic energy to specific areas of biological tissues as small as a few millimeters in diameter .…”

Section: Introductionmentioning

confidence: 99%

Focused Low-Intensity Pulsed Ultrasound Enhances Bone Regeneration in Rat Calvarial Bone Defect through Enhancement of Cell Proliferation

Jung

Kim

Ham

et al. 2015

Ultrasound in Medicine & Biology

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“…16 It is well known that LIPUS treatment stimulates osteogenesis, thus accelerating bone formation and repair. 17 Some studies have demonstrated that LIPUS stimulates the expression of cyclooxygenase 2 (COX-2) and runt-related transcription factor 2 (RUNX2), early response genes involved in the process of osteoblast differentiation. [18][19][20] Additionally, LIPUS has been shown to stimulate the expression of bone differentiation marker genes, including osteopontin, osteonectin, and osteocalcin, produced by osteoblasts.…”

Section: Discussionmentioning

confidence: 99%

Low-intensity pulsed ultrasound enhances bone morphogenetic protein expression of human mandibular fracture haematoma-derived cells

Huang

Hasegawa

Imai

et al. 2015

International Journal of Oral and Maxillofacial Surgery

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Bone regeneration by statin and low-intensity pulsed ultrasound (LIPUS) in rabbit nasal bone

Cited by 22 publications

References 50 publications

Low-intensity Pulsed Ultrasound Enhances Bone Repair in a Rabbit Model of Steroid-associated Osteonecrosis

Low-intensity Pulsed Ultrasound Enhances Bone Repair in a Rabbit Model of Steroid-associated Osteonecrosis

Focused Low-Intensity Pulsed Ultrasound Enhances Bone Regeneration in Rat Calvarial Bone Defect through Enhancement of Cell Proliferation

Low-intensity pulsed ultrasound enhances bone morphogenetic protein expression of human mandibular fracture haematoma-derived cells

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