Bone Regeneration Effect of Low Level Lasers Including Argon Laser

Nagasawa, Akinori; Kato, Kazuichi; Negishi, Akira

doi:10.5978/islsm.91-or-07

Cited by 35 publications

(13 citation statements)

References 2 publications

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“…The effect of low‐energy laser on the alveolar bone after tooth extraction showed increase in the deposition of bone (4). Radiological analysis after tooth extraction demonstrate that low intensity laser can activate repair of damaged bone tissue in patients (15).…”

Section: Introductionmentioning

confidence: 99%

Effect of low intensity laser irradiation on surgically created bony defects in rats

Nissan

Assif

Groß

et al. 2006

J of Oral Rehabilitation

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Low intensity lasers have been used by clinicians to improve healing and reduce pain in humans. Lasing also results in new bone formation around hydroxyapatite implants and a significant increase in the total bone area. However, the exact mechanism of cell biostimulation by laser is still unclear. This study biochemically assessed the effects of low intensity laser (Gallium-Arsenide) using 4 and 22.4 mW cm(-2) power density on the bone healing process after surgically creating bony cavities in rat mandibles. Rats (n = 24) were divided into two groups each treated with specific energy, 4 or 22.4 mW cm(-2), for 3 min each day post-surgery. Surgical cavities were created on both sides of the mandible: the left served as an untreated control, the right was treated with laser. All rats were sacrificed after 1, 2 and 4 weeks of treatment. In the newly formed callus, accumulation of radiocalcium and alkaline phosphatase activity was measured to indicate osteogenic activity. One-way anova with repeated measures showed that the low intensity laser using 4 mW cm(-2) power density significantly increased radiocalcium accumulation from 2 weeks post-surgery, whereas 22.4 mW cm(-2) had no effect. No changes were noted in the activity of alkaline phosphatase with the laser treatment. These results suggest that laser therapy of low power density is effective on the bone healing process in artificially created osseous cavities by affecting calcium transport during new bone formation.

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Section: Introductionmentioning

confidence: 99%

Effect of low intensity laser irradiation on surgically created bony defects in rats

Nissan

Assif

Groß

et al. 2006

J of Oral Rehabilitation

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“…In particular, the acceleration of bone regeneration by laser treatment has been investigated by in vivo study [7][8][9][10][11]. In vitro study, cell growth, DNA synthesis [12] and bone nodule formation [13] were significantly stimulated by low-level laser irradiation.…”

Section: Introductionmentioning

confidence: 99%

Stimulation of MCM3 Gene Expression in Osteoblast by Low Level Laser Irradiation

et al. 2001

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Biostimulatory effect of cell proliferation and bone formation by laser irradiation has been reported, however, very little is known about the molecular basis of mechanisms. We previously constructed the cDNA library of mouse osteoblastic cells (MC3T3-E1) which enhanced gene expression by laser irradiation using a subtracted gene cloning procedure. In the present study, we focused on a gene clone, designated as MCL-140, which exhibited the high homology of DNA sequence with mouse minichromosome maintenance (MCM) 3 gene. MCM3 is involved in the initiation of DNA replication as licensing factor in eukaryotic cells. Nucleotide sequence of MCL-140 insert was determined and assessed in the nucleic acid databases. The transcription level of MCL-140 was examined by Northern blot analysis. The DNA sequences of clone MCL-140 insert exhibited 96.2% homology with MCM 3 gene coding P1 protein. Higher MCM3 mRNA levels were observed in laser-irradiated cells compared to the levels in non-irradiated cells: furthermore, radiolabelled thymidine incorporation was increased by laser irradiation. These findings suggest that low-level laser irradiation may enhance DNA replication and play a role in stimulating proliferation of osteoblast through the enhancement of the MCM3 gene expression.

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“…Its application was initiated based on previous work that demonstrated properties of lowlevel laser that exert a positive influence on fibroblast 1 and osteoblast 2 proliferation, collagen synthesis, 3 and bone regeneration. 4 In vivo examinations have also shown that LLLT significantly stimulates the activity of alcalic phosphatase and calcium accumulation. 5 In addition to cartilage damage and bone metabolism, pathological alterations are also known to exhibit reduced circulation in the vessels of the joint parallel to the degenerative changes.…”

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confidence: 99%

The Effect of Low-Level Laser in Knee Osteoarthritis: A Double-Blind, Randomized, Placebo-Controlled Trial

Hegedüs

Viharos

Gervain³

et al. 2009

Photomedicine and Laser Surgery

171

118

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Introduction: Low-level laser therapy (LLLT) is thought to have an analgesic effect as well as a biomodulatory effect on microcirculation. This study was designed to examine the pain-relieving effect of LLLT and possible microcirculatory changes measured by thermography in patients with knee osteoarthritis (KOA). Materials and Methods: Patients with mild or moderate KOA were randomized to receive either LLLT or placebo LLLT. Treatments were delivered twice a week over a period of 4 wk with a diode laser (wavelength 830 nm, continuous wave, power 50 mW) in skin contact at a dose of 6 J=point. The placebo control group was treated with an ineffective probe (power 0.5 mW) of the same appearance. Before examinations and immediately, 2 wk, and 2 mo after completing the therapy, thermography was performed (bilateral comparative thermograph by AGA infrared camera); joint flexion, circumference, and pressure sensitivity were measured; and the visual analogue scale was recorded. Results: In the group treated with active LLLT, a significant improvement was found in pain (before treatment [BT]: 5.75; 2 mo after treatment : 1.18); circumference (BT: 40.45; AT: 39.86); pressure sensitivity (BT: 2.33; AT: 0.77); and flexion (BT: 105.83; AT: 122.94). In the placebo group, changes in joint flexion and pain were not significant. Thermographic measurements showed at least a 0.58C increase in temperature-and thus an improvement in circulation compared to the initial values. In the placebo group, these changes did not occur. Conclusion: Our results show that LLLT reduces pain in KOA and improves microcirculation in the irradiated area.

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Bone Regeneration Effect of Low Level Lasers Including Argon Laser

Cited by 35 publications

References 2 publications

Effect of low intensity laser irradiation on surgically created bony defects in rats

Effect of low intensity laser irradiation on surgically created bony defects in rats

Stimulation of MCM3 Gene Expression in Osteoblast by Low Level Laser Irradiation

The Effect of Low-Level Laser in Knee Osteoarthritis: A Double-Blind, Randomized, Placebo-Controlled Trial

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