Bone Regeneration in Osseous Defects Using Hydroxyapatite Graft and the Extent of Ossification in Osseous Defects Treated Without Grafts: A Comparative Evaluation

Shankar, Ravi; Singh, Deepak Kumar; Shaikh, Shoyeb; Singh, Gaurav; Yadav, Anurag; Jain, Ravi

doi:10.1007/s12663-011-0189-x

Cited by 9 publications

(6 citation statements)

References 10 publications

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“…Synthetic HA is a widely used bone substitute. It is a non-toxic compound with a similar chemical composition to human bone mineral [36,37]. It is traditionally regarded as an osteoconductive material [35], providing a mechanical framework to support bone repair.…”

Section: Discussionmentioning

confidence: 99%

Development and biological evaluation of fluorophosphonate-modified hydroxyapatite for orthopaedic applications

Neary

Blom

Shiel

et al. 2018

J Mater Sci: Mater Med

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There is an incentive to functionalise hydroxyapatite (HA) for orthopaedic implant use with bioactive agents to encourage superior integration of the implants into host bone. One such agent is (3S) 1-fluoro-3-hydroxy-4-(oleoyloxy) butyl-1-phosphonate (FHBP), a phosphatase-resistant lysophosphatidic acid (LPA) analogue. We investigated the effect of an FHBP-HA coating on the maturation of human (MG63) osteoblast-like cells. Optimal coating conditions were identified and cell maturation on modified and unmodified, control HA surfaces was assessed. Stress tests were performed to evaluate coating survivorship after exposure to mechanical and thermal insults that are routinely encountered in the clinical environment. MG63 maturation was found to be three times greater on FHBP-modified HA compared to controls (p < 0.0001). There was no significant loss of coating bioactivity after autoclaving (P = 0.9813) although functionality declined by 67% after mechanical cleaning and reuse (p < 0.0001). The bioactivity of modified disks was significantly greater than that of controls following storage for up to six months (p < 0.001). Herein we demonstrate that HA can be functionalised with FHBP in a facile, scalable manner and that this novel surface has the capacity to enhance osteoblast maturation. Improving the biological performance of HA in a bone regenerative setting could be realised through the simple conjugation of bioactive LPA species in the future. Depicted is a stylised summary of hydroxyapatite (HA) surface modification using an analogue of lysophosphatidic acid, FHBP. a HA surfaces are simply steeped in an aqueous solution of 2 μM FHBP. b The polar head group of some FHBP molecules react with available hydroxyl residues at the mineral surfaces forming robust HA-O-P bonds leaving acyl chain extensions perpendicular to the HA surface. These fatty acyl chains provide points of integration for other FHBP molecules to facilitate their self-assembly. This final surface finish enhanced the human osteoblast maturation response to calcitriol, the active vitamin D3 metabolite.

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Section: Discussionmentioning

confidence: 99%

Development and biological evaluation of fluorophosphonate-modified hydroxyapatite for orthopaedic applications

Neary

Blom

Shiel

et al. 2018

J Mater Sci: Mater Med

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“…In addition, this biomaterial is atoxic and has a controllable microstructure in terms of pore size 6 . In view of its osteogenic properties, hydroxyapatite is widely used in bone reconstruction surgeries [7][8][9] . Another material used mainly for the regeneration of soft tissues is fibrin sealant, which serves as a scaffold for cell adhesion and growth, in addition to presenting hemostatic properties.…”

Section: Introductionmentioning

confidence: 99%

Hydroxyapatite and a New Fibrin Sealant Derived from Snake Venom as Scaffold to Treatment of Cranial Defects in Rats

et al. 2015

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Biomaterials are used as a promising alternative to bone grafts, including bioceramics whose composition resembles that of bone and fibrin sealants due to their hemostatic properties. The objective was to evaluate the repair of cranial defects in 40 rats, grafted with hydroxyapatite and a new fibrin sealant derived from snake venom. The animals were divided into four groups: C (control, no graft); Ha (hydroxyapatite); FS (fibrin sealant), and HaFS (hydroxyapatite and fibrin sealant). The animals were euthanized 2 and 6 weeks after surgery and wound area were submitted to analysis. After 2 weeks, immature bone was formed from the borders of the defect and in groups Ha and HaFS, few hydroxyapatite particles were surrounded by new bone. After 6 weeks, the new bone was mature and surrounded several hydroxyapatite particles, without connective tissue interposition and the volume of new bone was higher in HaFS group. The hydroxyapatite in combination with the new fibrin sealant accelerates bone repair.

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“…Artificial hydroxyapatite proves to be complete absorbable leaving new bone formation only. It is non-toxic, has a high chemical stability and causes less inflammatory and allergic reactions (Shankar et al 2011). Dense nonabsorbable HA particles are used in long-term augmentation of severely resorbed alveolar ridges (Schaffer 1993).…”

mentioning

confidence: 99%

Monophasic ß‐TCP vs. biphasic HA/ß‐TCP in two‐stage sinus floor augmentation procedures – a prospective randomized clinical trial

Jelušić¹,

Zirk

Fienitz

et al. 2016

Clinical Oral Implants Res

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Bone Regeneration in Osseous Defects Using Hydroxyapatite Graft and the Extent of Ossification in Osseous Defects Treated Without Grafts: A Comparative Evaluation

Cited by 9 publications

References 10 publications

Development and biological evaluation of fluorophosphonate-modified hydroxyapatite for orthopaedic applications

Development and biological evaluation of fluorophosphonate-modified hydroxyapatite for orthopaedic applications

Hydroxyapatite and a New Fibrin Sealant Derived from Snake Venom as Scaffold to Treatment of Cranial Defects in Rats

Monophasic ß‐TCP vs. biphasic HA/ß‐TCP in two‐stage sinus floor augmentation procedures – a prospective randomized clinical trial

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