Bone Regulatory Mechanisms Destruction in Experimental Chronic Kidney Disease

Pavlov, S. B.; Кумечко, М В; Litvinova, O. B.; Babenko, Nataliia; Goncharova, A. V.

doi:10.15407/fz62.03.054

Cited by 6 publications

(3 citation statements)

References 13 publications

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“…It should also be borne in mind that, inflammation can be one of the factors associated with a decrease in bone mass. (25) A decrease in inflammation appears to lead to a decrease in resorption processes. The results of the histological study carried out in our work confirm the improvement of healing in the group of animals, which was modeled fracture of the proximal femur and…”

Section: Bioactive Molecules and Biochemical Parametersmentioning

confidence: 99%

The Enalapril Use in Arterial Hypertension Stimulates The Reparative Processes in Fractures of The Proximal Femur

et al. 2022

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BACKGROUND: In patients with a fracture of the proximal femur and concomitant arterial hypertension, there is a disturbance of the reparative processes of bone tissue. This research aimed to study the regulation of the reparative processes of fractures of the proximal femur with intramedullary osteosynthesis during the correction of concomitant hypertension, which was examined based on some markers using the rat model.METHODS: The study involved healthy Wistar rats and spontaneously hypertensive rats (SHR). The subjects were then grouped into healthy rats without exposure (1.1) SHR without exposure (2.1), healthy rats with modeled fractures of the proximal femur (1.2), SHR with modeled fractures of the proximal femur (2.2), SHR underwent hypertension correction with enalapril in subgroups without fracture (2.3) and SHR underwent hypertension correction with enalapril in subgroups with fracture (2.4). The levels of interleukin (IL)-6, tumor necrosis factor alpha (TNF-a), IL-10, amino-terminal propeptide procollagen type III (PIIINP), glucose, uric acid, creatinine, urea, cholesterol, and albumin were determined in the blood serum of the animals. Femur preparations were examined after the removal of intramedullary fixation. RESULTS: Serum IL-6 level of animal in group 2.4 (2.297±0.361 pg/mL) were reduced compared to the corresponding indicators of rats in group 2.3 (4.054±0.491 pg/mL, p<0.05). Serum glucose and urea levels of animal in group 2.4 (3.951±0.156 mmol/L, 6.552±0.426 mmol/L, respectively) were significantly reduced in comparison with the group 2.3 (6.384±0.890 mmol/L, 10.369±0.888 mmol/L, respectively). The histological results indicated a positive effect of the drug enalapril on the healing of fractures of the proximal femur in animals with hypertension.CONCLUSION: Correction of arterial hypertension with enalapril in fractures of the proximal femur improves the reparative processes of bone tissue.KEYWORDS: injury healing, remodeling, concomitant diseases, angiotensin-converting enzyme inhibitors, cytokines, growth factor, collagen, biochemical parameters

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Section: Bioactive Molecules and Biochemical Parametersmentioning

confidence: 99%

The Enalapril Use in Arterial Hypertension Stimulates The Reparative Processes in Fractures of The Proximal Femur

et al. 2022

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“…With the development of chronic inflammation, an imbalance of regulatory mechanisms occurs, characterised by the activity of proinflammatory cytokines and the insufficient effect of their inhibitors and antagonists. 7 Prolongation or delay of the inflammatory phase negatively affects the remaining stages of wound healing. 8 During the proliferative phase, granulation tissue formation, re-epithelialisation and angiogenesis occur.…”

Section: Introductionmentioning

confidence: 99%

Features of metabolism in chronic wound remodelling

Pavlov,

Babenko,

Kumetchko

et al. 2024

Scripta Medica

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Background/Aim: The treatment of chronic wounds continues to be a pressing problem throughout the world. Healing occurs through some evolutionarily conserved biochemical pathways. The mechanisms of development of disorders of reparative regeneration are not fully understood. The work aimed to study the dynamics of changes in metabolic parameters during the healing of chronic wounds. Methods: Healthy Wistar rats were divided into two groups. The animals of the first group were intact. Chronic wounds were simulated for the animals of the second group. On days 7, 14 and 28 after wound creation, the animals were euthanised. Biochemical parameters such as glucose, total protein, albumin, cholesterol, urea, creatinine, aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) were assessed in the blood serum of animals. Results: It was found that the maximum decrease in glucose and total protein levels in the blood serum of animals in the experimental groups compared to intact animals was observed 2 weeks after surgery: the glucose concentration in rats was 1.7 times lower (p < 0.001). The level of albumin in the blood serum of experimental animals compared to intact animals was reduced by 1.5 times after 14 days (p < 0.001) and by 1.2 times after 28 days (p < 0.01). A week after surgery, the concentration of urea in the blood serum of experimental animals was 1.3 times higher (p < 0.01) than in intact rats and by day 28 after surgery, the urea level was 1.4 times higher (p < 0.001). The reduction in cholesterol and creatinine levels was not significant. An increase in AST, AST and ALP levels in the blood serum of experimental animals was shown. An increase in the blood serum of animals 7 days after surgery compared to the indicators of intact animals: ALP concentrations by 2.8 times (p < 0.001) and ALT concentrations by 1.4 times (p < 0.001) was established. The AST level significantly increased 14 days after surgery (p < 0.05). Conclusions: The study of metabolic parameters allows monitoring of the state of the body during the healing process of wounds to correct treatment tactics.

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“…The clinical outcome of the disease is associated precisely with the reaction of the connective tissue, as a physiological system, as one of the most important components of the systemic inflammatory response syndrome [3]. For elderly patients, this becomes critically important, not only because we are talking about bone damage in the background of osteoporosis, but primarily because the physiological reserves of the body are significantly reduced.…”

Section: Introductionmentioning

confidence: 99%

Bone remodeling features in elderly and senile patients with the proximal femur fractures after hip replacement

et al. 2020

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The aim of this study is to identify the dependence of the result of surgical treatment of patients of elderly and senile age with fractures of the proximal femur on the characteristics of the response cytokine-mediated regulatory response to trauma and surgery. Materials and methods: In 74 patients after hip arthroplasty, serum levels of bone metabolism markers were determined using enzyme-linked immunosorbent assay. Patients were divided into 2 groups depending on the results of treatment. Results: It was found that compared with group 2 (treatment outcome is worse) in group 1 (treatment outcome is better) there was a greater number of correlations. In group 1, correlations were found between OPG and RANKL (r = 0.88; p = 0.000), OPG and OPG/RANKL (r = 0.44; p = 0.006), TGF-β1 and OPG/RANKL (r = 0.66; p = 0.000) , IL-6 and OPG (r = 0.67; p = 0.000), IL-6 and RANKL (r = 0.53; p = 0.001), IL-6 and OPG/RANKL (r = 0.39; p = 0.016). In group 2, only between OPG and OPG/RANKL (r = 0.72; p = 0.000), RANKL and OPG/RANKL (r = −0.53; p = 0.0007). In patients of group 2, there was a decrease in the level of OPG relative to the control and a less significant increase in TGF-β1 and IL-6 relative to group 1. Conclusion: The prognosis of the results of treatment of patients with proximal femur fractures is largely determined by the nature of the adaptive response to injury and the implant, the synchronism of the mechanism of stress remodeling of the bone. A less favorable prognosis after arthroplasty is associated with exacerbation of the initial metabolic disorders in the bone tissue due to severe cytokine-mediated dysfunction of the regulatory pathways.

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Bone Regulatory Mechanisms Destruction in Experimental Chronic Kidney Disease

Abstract: Метою нашої роботи було вивчення ролі міжклітинних медіаторів -рецепторного антагоніста інтерлейкіну-1 (ІЛ-1 РA), інтерлейкіну-17 (ІЛ-17)

Cited by 6 publications

References 13 publications

The Enalapril Use in Arterial Hypertension Stimulates The Reparative Processes in Fractures of The Proximal Femur

The Enalapril Use in Arterial Hypertension Stimulates The Reparative Processes in Fractures of The Proximal Femur

Features of metabolism in chronic wound remodelling

Bone remodeling features in elderly and senile patients with the proximal femur fractures after hip replacement

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