Bone Sialoprotein, Matrix Metalloproteinase 2, and  v 3 Integrin in Osteotropic Cancer Cell Invasion

Karadağ, Abdullah; Ogbureke, Kalu U.E.; Fedarko, Neal S.; Fisher, Larry W.

doi:10.1093/jnci/djh169

Cited by 104 publications

(109 citation statements)

References 43 publications

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“…The formation of this complex leads to MMP activation and is thought to be important for invading cancer cells. 67 Furthermore, ON has been reported to induce activation of MMP2 and to regulate MMP9 expression in vivo. 20,68 Clearly, in our model extravasation of pancreatic cancer cells is a step which precedes their growth in the liver of nude rats.…”

Section: Discussionmentioning

confidence: 99%

Osteopontin but not osteonectin favors the metastatic growth of pancreatic cancer cell lines

Zhivkova-Galunska

Adwan²,

Eyol³

et al. 2010

Cancer Biology & Therapy

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Section: Discussionmentioning

confidence: 99%

Osteopontin but not osteonectin favors the metastatic growth of pancreatic cancer cell lines

Zhivkova-Galunska

Adwan²,

Eyol³

et al. 2010

Cancer Biology & Therapy

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“…BSP promoted invasion of several osteotropic cancer cell lines in vitro by apparently localizing MMP2 to the cell surface through αvβ3 (REF. 55). DMP1 enhanced the invasion potential of a colon cancer cell line by bridging MMP9 to integrins and, perhaps, CD44 (REF.…”

Section: Invasion and Extracellular Matrix Degradationmentioning

confidence: 99%

Small integrin-binding ligand N-linked glycoproteins (SIBLINGs): multifunctional proteins in cancer

et al. 2008

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Numerous components and pathways are involved in the complex interplay between cancer cells and their environment. The family of glycophosphoproteins comprising osteopontin, bone sialoprotein, dentin matrix protein 1, dentin sialophosphoprotein and matrix extracellular phosphoglycoprotein -small integrin-binding ligand N-linked glycoproteins (SIBLINGs) -are emerging as important players in many stages of cancer progression. From their detection in various human cancers to the demonstration of their key functional roles during malignant transformation, invasion and metastasis, the SIBLINGs are proteins with potential as diagnostic and prognostic tools, as well as new therapeutic targets.

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“…The cleaved lower molecular weight osteopontin fragments can penetrate into cells and have biological activities in promoting both cell adhesion and migration, but these are not observed for the intact osteopontin molecule (38). Thus, it was proposed that cleaved osteopontin may be a member of the SIBLING family of small integrin-binding ligand N-linked glycoproteins that can increase invasiveness of cancer cells by interacting with their specific matrix metalloproteinase and integrin partners, which was shown by a recent in vitro study (40). Our semiquantitative data clearly showed that COOHterminal osteopontin fragments were strongly correlated with ovarian cancer stages or invasiveness and support the previous findings.…”

Section: Discussionmentioning

confidence: 99%

Proteomic-Based Discovery and Characterization of Glycosylated Eosinophil-Derived Neurotoxin and COOH-Terminal Osteopontin Fragments for Ovarian Cancer in Urine

Skates²,

Mok

et al. 2006

Clinical Cancer Research

149

115

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Purpose: The objective was to identify and characterize low molecular weight proteins/peptides in urineandtheirposttranslationalmodifications thatmightbeusedas ascreeningtoolforovariancancer. Experimental Design: Urine samples collected preoperatively from postmenopausal women with ovarian cancer and benign conditions and from nonsurgical controls were analyzed by surface-enhanced laser desorption/ionization mass spectrometry and two-dimensional gel electrophoresis. Selected proteins from mass profiles were purified by chromatography and followed by liquid chromatography-tandem mass spectrometry sequence analysis. Specific antibodies were generated for further characterization, including immunoprecipitation and glycosylation. Quantitative and semiquantitative ELISAs were developed for preliminary validation in patients of 128 ovarian cancer, 52 benign conditions, 44 other cancers, and 188 healthy controls. Results: A protein (m/z f17,400) with higher peak intensities in cancer patients than in benign conditions and controls was identified and subsequently defined as eosinophil-derived neurotoxin (EDN). A glycosylated form of EDN was specifically elevated in ovarian cancer patients. A cluster of COOH-terminal osteopontin was identified from two-dimensional gels of urine from cancer patients. Modified forms EDN and osteopontin fragments were elevated in early-stage ovarian cancers and a combination of both resulted to 93% specificity and 72% sensitivity. Conclusions: Specific elevated posttranslationally modified urinary EDN and osteopontin COOH-terminal fragments in ovarian cancer might lead to potential noninvasive screening tests for early diagnosis. Urine with less complexity than serum and relatively high thermodynamic stability of peptides or metabolites is a promising study medium for discovery of the novel biomarkers which may present in many nonurinary tract neoplastic diseases.

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Bone Sialoprotein, Matrix Metalloproteinase 2, and v 3 Integrin in Osteotropic Cancer Cell Invasion

Abstract: Cancer cells appear to become more invasive when BSP forms a cell-surface trimolecular complex by linking MMP-2 to integrin alpha(v)beta3.

Cited by 104 publications

References 43 publications

Osteopontin but not osteonectin favors the metastatic growth of pancreatic cancer cell lines

Osteopontin but not osteonectin favors the metastatic growth of pancreatic cancer cell lines

Small integrin-binding ligand N-linked glycoproteins (SIBLINGs): multifunctional proteins in cancer

Proteomic-Based Discovery and Characterization of Glycosylated Eosinophil-Derived Neurotoxin and COOH-Terminal Osteopontin Fragments for Ovarian Cancer in Urine

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