Bone tissue engineering electrospun scaffolds based on layered double hydroxides with the ability to release vitamin D3: Fabrication, characterization and in vitro study

Belgheisi, Ghazal; Nazarpak, Masoumeh Haghbin; Solati‐Hashjin, Mehran

doi:10.1016/j.clay.2019.105434

Cited by 36 publications

(21 citation statements)

References 31 publications

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“…3b) indicates that the pores do not significantly affect the rate of nutrient spread. Such behavior at similar parameters is confirmed in experimental works [42,[46][47][48] and possibly indicates the selection of the optimal scaffold rotation rate. At rotation rates of 24 rpm (Fig.…”

Section: Results Of Internal Flow Modelingsupporting

confidence: 66%

A Numerical Study of Fluid Flow in the Porous Structure of Biological Scaffolds

Daulbayev

Mansurov

Sultanov

et al. 2020

Eurasian Chem. Tech. J.

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Tissue engineering (TE) is one of the promising areas that aims to address the global problem of organ and tissue shortages. The successful development of TE, particularly in bone tissue engineering, consists of the use of modern methods that allow the creation of scaffolds, the physicochemical, mechanical, and structural parameters of which will allow achieving the desired clinical results. The vast possibilities of the rapidly developing technology of three-dimensional (3D) printing, which allows the creation of individual scaffolds with high precision, has led to various developments in bone tissue TE. In this work, for the successful use of three-dimensional printing in TE to ensure the diffusion of nutrients during cell cultivation throughout the entire structure of the scaffold, a model of a rotating scaffold is proposed, and the movement of the diffusion flow of nutrient fluid is calculated based on Darcy’s law, which regulates the flow of fluids through porous media. The conducted studies of the rate of diffusion flow of nutrients based on glucose in the porous structure of scaffolds with a 10% content of calcium hydroxyapatite demonstrated the promise of using a model of a rotating composite scaffold in TE of bone tissue. The results show that at a scaffold rotation speed of 12 rpm, the diffusion flow rate of nutrients in the composite scaffolds porous structure is practically not affected by their geometric shape.

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Section: Results Of Internal Flow Modelingsupporting

confidence: 66%

A Numerical Study of Fluid Flow in the Porous Structure of Biological Scaffolds

Daulbayev

Mansurov

Sultanov

et al. 2020

Eurasian Chem. Tech. J.

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“…Furthermore, it seems that VD, HA, and gelatin can affect not Fig. 5 a In vitro release of VD from scaffolds at specified time intervals; b In vitro release of VD from scaffolds at early hours; while comparing the profiles with mathematical kinetic models, it was observed that they were not matched with any model and exhibited different behavior only the bone formation process in vivo but also the formation of secondary apatite crystals in vitro [30,42]. As for the GHG1 that was decomposed entirely after 14 days, it can be said that although the time of degradation may be earlier than the completion of the bone healing, this may encourage cells to accelerate the healing process, because if the scaffold remains for a long time, cells may complete regeneration process slower, and scaffold debris can cause local side effects.…”

Section: In Vitro Studiesmentioning

confidence: 99%

Bone tissue engineering gelatin–hydroxyapatite/graphene oxide scaffolds with the ability to release vitamin D: fabrication, characterization, and in vitro study

Mahdavi

Belgheisi

Haghbin-Nazarpak

et al. 2020

J Mater Sci: Mater Med

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Developing smart scaffolds with drug release capability is one of the main approaches to bone tissue engineering. The current study involves the fabrication of novel gelatin (G)-hydroxyapatite (HA)-/vitamin D (VD)-loaded graphene oxide (GO) scaffolds with different concentrations through solvent-casting method. Characterizations confirmed the successful synthesis of HA and GO, and VD was loaded in GO with 36.87 ± 4.87% encapsulation efficiency. Physicochemical characterizations showed that the scaffold containing 1% VD-loaded GO had the best mechanical properties and its porosity percentage and density was in the range of natural spongy bone. All scaffolds were degraded after 1-month, subjecting to phosphate buffer saline. The release profile of VD did not match any mathematical kinetics model, porosities and the degradation rate of the scaffolds were dominant controlling factors of release behavior. Studies on the bioactivity of scaffolds immersed in simulated body fluid indicated that VD and HA could encourage the formation of secondary apatite crystals in vitro. Buccal fat pad-derived stem cells (BFPSCs) were seeded on the scaffolds, MTT assay, alkaline phosphatase activity as an indicator of osteoconductivity, and cell adhesion were conducted in order to evaluate in vitro biological responses. All scaffolds highly supported cell adhesion, MTT assay indicated better cell viability in 0.5% VD-loaded GO containing scaffold, and the scaffold enriched with 2% VD-loaded GO performed the most ALP activity. The results demonstrated the potential of these scaffolds to induce bone regeneration.

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“…Figure 1(c) (Vit D3) confirmed this result. 19,33,34 According to the Figure 1(b), the peaks around 1722, 1287, 1380and 1450 cm À1 are related to the carbonyl, ester and alkyl functional groups of PHB. 23 On the other hand, the bonds around 1624 and 1707 cm À1 related to the carboxyl groups of ciprofloxacin.…”

Section: Structural Analysismentioning

confidence: 98%

“…Other researcher revealed that that the release of the vitamin D3 showed the similar pattern to our membrane as the main controlling mechanism of release. 19…”

Section: In Vitro Drug Release Studymentioning

confidence: 99%

“…15 The bioceramics and vitamin D3 have been used to improve the properties of the polymer and promote bone tissue. [16][17][18][19] Beta-tri-calcium phosphate has been used for medical applications due to its excellent tensile and compressive strength as well as high biocompatibility. 20 In addition, the infection control is one of the necessary criteria for barrier membranes.…”

Section: Introductionmentioning

confidence: 99%

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Preparation and evaluation of bioactive bilayer composite membrane PHB/β‐TCP with ciprofloxacin and vitamin D3 delivery for regenerative damaged tissue in periodontal disease

Tayebi

Parham

Ahangar

et al. 2021

J of Applied Polymer Sci

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Periodontal tissue engineering has made a tremendous change in the treatment of periodontal disease. In this study, electrophoresed poly-hydroxy-butyrate (PHB) scaffold, beta-three calcium phosphate (β-TCP) nano powder in 3, 5 and 7% wt, ciprofloxacin antibiotics, and vitamin D3 as the major materials along with electrospinning method are selected for remaking the scaffold. The fibers are smooth and beads-free and have a good diameter (around 700 nm) and porosity (above 80%) indicates ideal conditions for the treatment of damaged tissue. The membrane with 5% ceramic has been selected as ideal membrane. The mention membrane has suitable mechanical properties. Studies have shown that adding ciprofloxacin to the membrane increases the rate of hydrophilicity. The addition of β-TCP nano powder increased the bioactivity of this membrane. In terms of the drug release of ciprofloxacin and vitamin D3, 45% and 60% of drugs released within the first 24 h which in turn it shows the ability of the drugs controlled deliveration by the membrane. Accordingly, it could be concluded that this bilayer composite membrane with high bioactivity, high hydrophilicity, suitable mechanical properties, suitable morphology, and appropriate porosity has the ideal conditions for increased bone growth as well as proliferation and treatment the periodontal disease.

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Bone tissue engineering electrospun scaffolds based on layered double hydroxides with the ability to release vitamin D3: Fabrication, characterization and in vitro study

Cited by 36 publications

References 31 publications

A Numerical Study of Fluid Flow in the Porous Structure of Biological Scaffolds

A Numerical Study of Fluid Flow in the Porous Structure of Biological Scaffolds

Bone tissue engineering gelatin–hydroxyapatite/graphene oxide scaffolds with the ability to release vitamin D: fabrication, characterization, and in vitro study

Preparation and evaluation of bioactive bilayer composite membrane PHB/β‐TCP with ciprofloxacin and vitamin D3 delivery for regenerative damaged tissue in periodontal disease

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