Boosting extracellular vesicle secretion

Debbi, Lior; Guo, Shengming; Shulamit, Dina Safina; Levenberg,

doi:10.1016/j.biotechadv.2022.107983

Cited by 90 publications

(66 citation statements)

References 58 publications

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“…Compared to 2D culture, static 3-D culture showed an increased EV biogenesis, revealing the contribution of 3D architecture [ 46 ]. Compared to static 3-D culture, dynamic 3-D culture also increased the EV biogenesis, showing the contribution of the hydrodynamics of bioreactors [ 40 , 60 ].…”

Section: Discussionmentioning

confidence: 99%

Engineering Human Mesenchymal Bodies in a Novel 3D-Printed Microchannel Bioreactor for Extracellular Vesicle Biogenesis

et al. 2022

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Human Mesenchymal Stem Cells (hMSCs) and their derived products hold potential in tissue engineering and as therapeutics in a wide range of diseases. hMSCs possess the ability to aggregate into “spheroids”, which has been used as a preconditioning technique to enhance their therapeutic potential by upregulating stemness, immunomodulatory capacity, and anti-inflammatory and pro-angiogenic secretome. Few studies have investigated the impact on hMSC aggregate properties stemming from dynamic and static aggregation techniques. hMSCs’ main mechanistic mode of action occur through their secretome, including extracellular vesicles (EVs)/exosomes, which contain therapeutically relevant proteins and nucleic acids. In this study, a 3D printed microchannel bioreactor was developed to dynamically form hMSC spheroids and promote hMSC condensation. In particular, the manner in which dynamic microenvironment conditions alter hMSC properties and EV biogenesis in relation to static cultures was assessed. Dynamic aggregation was found to promote autophagy activity, alter metabolism toward glycolysis, and promote exosome/EV production. This study advances our knowledge on a commonly used preconditioning technique that could be beneficial in wound healing, tissue regeneration, and autoimmune disorders.

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Section: Discussionmentioning

confidence: 99%

Engineering Human Mesenchymal Bodies in a Novel 3D-Printed Microchannel Bioreactor for Extracellular Vesicle Biogenesis

et al. 2022

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“…In fact, studies have shown that there were a variety of ways to promote exosome production by cells in situ, including stress or injury to cells, regulating small GTPases, heat shock protein 90, cortactin, and Ca 2+ (like monensin). [ 36 ] Therefore, in order to make better use of exosome‐mediated deep tumor penetration, the combination of promoting in situ generation of exosomes and drug delivery may be the potential way for future research.…”

Section: Exosomesmentioning

confidence: 99%

Organism‐Generated Biological Vesicles In Situ: An Emerging Drug Delivery Strategy

2022

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Biological vesicles, containing genetic materials and proteins of the original cells, are usually used for local or systemic communications among cells. Currently, studies on biological vesicles as therapeutic strategies or drug delivery carriers mainly focus on exogenously generated biological vesicles. However, the limitations of yield and purity caused by the complex purification process still hinder their clinical transformation. Recently, it has been reported that living organisms, including cells and bacteria, can produce functional/therapeutic biological vesicles within body automatically. Therefore, using organisms to produce endogenous biological vesicles in body as drug/bio‐information delivery carriers has become a potential therapeutic strategy. In this review, the current development status and application prospects of in situ organism‐produced biological vesicles are introduced. The advantages and effects of this endogenous biological vesicles‐based strategy in drug delivery and disease treatments are analyzed. According to the type of endogenous biological vesicles, they are divided into four categories: exosomes, platelet‐derived microparticles, apoptotic bodies, and bacteria‐released outer membrane vesicles. And finally, the shortcomings of current research and future development are analyzed. This review is believed to open up the application of endogenous biological vesicles in the field of biomedicine and shed light on current research.

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“…Finally, advances in generating thyroid organoids could also help and be inspiring for thyroid-derived EV functional studies [ 89 , 90 ]. A recent review also highlighted the possibility of boosting EV production using a 3D culture system, among other possibilities [ 91 ]. Finally, excessive dosages with questionable physiological relevance are often used in in vitro assays [ 92 , 93 ].…”

Section: Concerns and Further Directionsmentioning

confidence: 99%

Role of Extracellular Vesicles in Thyroid Physiology and Diseases: Implications for Diagnosis and Treatment

2022

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Extracellular vesicles are spherical subcellular structures delimited by a lipid bilayer and released by most cells in the human body. They are loaded with a myriad of molecules (i.e., nucleic acids and proteins) depending on their cell of origin and provide the ability to transmit a message to surrounding or distant target cells. In several organs, including the thyroid, abundant recent literature reports that extracellular vesicles are responsible for intercellular communication in physiological and pathological processes, and that their utilization as a potential biomarker of pathological states (i.e., cancer, autoimmune diseases) or as therapeutic delivery vehicles promise clinical options. In this review, we present the current knowledge and understanding regarding the role of extracellular vesicles in developing thyroid diseases and diagnosis.

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Boosting extracellular vesicle secretion

Cited by 90 publications

References 58 publications

Engineering Human Mesenchymal Bodies in a Novel 3D-Printed Microchannel Bioreactor for Extracellular Vesicle Biogenesis

Engineering Human Mesenchymal Bodies in a Novel 3D-Printed Microchannel Bioreactor for Extracellular Vesicle Biogenesis

Organism‐Generated Biological Vesicles In Situ: An Emerging Drug Delivery Strategy

Role of Extracellular Vesicles in Thyroid Physiology and Diseases: Implications for Diagnosis and Treatment

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