Boosting Polyamines to Enhance Shoot Regeneration in Potato (Solanum tuberosum L.) Using AgNO3

Abstract

Advancements in shoot regeneration systems support biotechnology-based tools used in the genetic improvement of plant crops. This study aims to enhance shoot regeneration in potatoes by boosting polyamine content by adding AgNO3 to the shoot regeneration medium (MS medium supplemented with 30 g L−1 sucrose, 100 mg L−1 myoinositol, and 2.25 BA mg L−1). Five concentrations of AgNO3 (2, 4, 6, 8, and 10 mg L−1) were used in addition to a control. The effect of AgNO3 on regeneration assumed a more or less concentration-dependent bell-shaped curve peaking at 4 mg L−1. Enhancements in shoot regeneration were attributed to the known role of AgNO3 as an ethylene action blocker in addition to improvements in polyamine accumulation without an increase in H2O2 content, lipid peroxidation, or DNA damage. The uncoupling of shoot regeneration and polyamine content recorded at high AgNO3 concentrations can be attributed to the consumption of polyamines to counteract the synchronized oxidative stress manifested by increases in H2O2 content, lipid peroxidation, and DNA damage.

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“…In addition, Frukh et al [45] observed that the accumulation of H 2 O 2 generated more MDA in rice plants under salinity stress. The relationship of an increase in H 2 O 2 content and MDA accumulation was documented by several research groups [46][47][48].…”

Somaclonal Variation for Genetic Improvement of Starch Accumulation in Potato (Solanum tuberosum) Tubers

“…In the current study, callus was induced on internode explants on a medium supplemented with 2.25 mg L −1 BAP and 0.186 mg L −1 NAA, while shoot regeneration was achieved following auxin removal and the addition of AgNO 3 at 4 mg L −1 . The same media were utilized for callus induction followed by shoot regeneration from potato leaves and internode explants in a previous study [ 32 ]. Similarly, Kumlay and Ercisli [ 33 ] employed a medium supplemented with both cytokinin and auxin to induce callus formation on the leaves and internode explants of four potato cultivars, whereas shoot regeneration commenced following auxin omission.…”

“…The role played by AgNO 3 in shoot regeneration media can be attributed to the intrusion with ethylene perception, which mitigates the hindering influence of the accretion of the gas hormone on shoot regeneration [ 38 , 39 ]. In addition, AgNO 3 enhances the accumulation of polyamines [ 32 ], whose role in the improvement of morphogenesis has been previously recorded in potato shoot cultures [ 40 ].…”

“…Based on our previous publication [ 32 ], about 1 cm of each of the internode explants was utilized for callus induction on a basal medium augmented with 2.25 mg L −1 BAP and 0.186 mg L −1 NAA. Six weeks later, the explants carrying calli were transferred to a regeneration medium consisting of a basal medium supplemented with 2.25 mg L −1 BAP and 4 mg L −1 AgNO 3 .…”

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“…AgNO 3 has been widely applied in fields such as agriculture [ 23 , 26 ], medical science [ 27 ], and environmental management [ 28 ]. It is primarily used in agriculture to achieve a range of results, such as plant growth and development control, high-quality plants, and flowering control [ [29] , [30] , [31] ]. Although the potential of AgNO 3 is widely accepted, its use in agriculture to increase secondary metabolite levels in plants remains debated [ 23 ].…”