Boron doped diamond and glassy carbon electrodes comparative study of the oxidation behaviour of cysteine and methionine

Enache, Teodor Adrian; Oliveira-Brett, Ana Maria

doi:10.1016/j.bioelechem.2011.02.001

Cited by 142 publications

(92 citation statements)

References 23 publications

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“…Cys yielded the poorest selectivity because it is electroactive; however, when channel subtraction (self-referencing) between glutamate oxidase-coated sites and sentinel sites was applied, the selectivity improved to 250 ± 80. Others have previously reported the direct oxidation and quantification of amino acids: Arg, 18,19 Cys, 20,21 Met, 20 Trp, 22−24 and Tyr. 24 The control sites were less sensitive (83 ± 7% of glutamate response for Arg, Cys, His, Met, Trp, and Tyr), possibly because of coating differences between the control and glutamate sensitive sites.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Glutaraldehyde Cross-Linked Glutamate Oxidase Coated Microelectrode Arrays: Selectivity and Resting Levels of Glutamate in the CNS

Burmeister

Davis

Quintero

et al. 2013

ACS Chem. Neurosci.

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Glutaraldehyde is widely used as a cross-linking agent for enzyme immobilization onto microelectrodes. Recent studies and prior reports indicate changes in enzyme activity and selectivity with certain glutaraldehyde cross-linking procedures that may jeopardize the performance of microelectrode recordings and lead to falsely elevated responses in biological systems. In this study, the sensitivity of glutaraldehyde cross-linked glutamate oxidase-based microelectrode arrays to 22 amino acids was tested and compared to glutamate. As expected, responses to electroactive amino acids (Cys, Tyr, Trp) were detected at both nonenzyme-coated and enzyme-coated microelectrodes sites, while the remaining amino acids yielded no detectable responses. Electroactive amino acids were effectively blocked with a m-phenylene diamine (mPD) layer and, subsequently, no responses were detected. Preliminary results on the use of poly(ethylene glycol) diglycidyl ether (PEGDE) as a potentially more reliable cross-linking agent for the immobilization of glutamate oxidase onto ceramic-based microelectrode arrays are reported and show no significant advantages over glutaraldehyde as we observe comparable selectivities and responses. These results support that glutaraldehyde-cross-linked glutamate oxidase retains sufficient enzyme specificity for accurate in vivo brain measures of tonic and phasic glutamate levels when immobilized using specific "wet" coating procedures.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Glutaraldehyde Cross-Linked Glutamate Oxidase Coated Microelectrode Arrays: Selectivity and Resting Levels of Glutamate in the CNS

Burmeister

Davis

Quintero

et al. 2013

ACS Chem. Neurosci.

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“…low background current, wide potential window, high stability combined with biocompatibility and chemical inertness makes it a very promising material for the third-generation biosensors [19,20]. The fast response, sensitivity and selectivity of biosensors based on BBD electrodes aroused great interest among scientists [3,[21][22][23][24][25][26]. The BDD electrodes are mostly prepared by the microwave plasma enhanced-chemical vapor deposition (MW PE CVD) method, while the as-deposited surfaces of BDD are hydrogen terminated (H-BDD).…”

Section: Introductionmentioning

confidence: 99%

Poly-l-lysine-modified boron-doped diamond electrodes for the amperometric detection of nucleic acid bases

Niedziałkowski

Ossowski

Zięba

et al. 2015

Journal of Electroanalytical Chemistry

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“…Methionine oxidation occurs in two steps, involving the adsorption and the protonation/deprotonation of the thiol group [12,14]. Histidine oxidation occurs in a single step at a higher positive potential than the other electroactive amino acids in the same experimental conditions [12,13,15].…”

Section: Introductionmentioning

confidence: 99%

“…The oxidation occurs in the electroactive amino acids [10][11][12]. Among the 20 amino acids presented in proteins, only tyrosine (Tyr), tryptophan (Trp), histidine (His) [12,13], cysteine (Cys) and methionine (Met) residues [12,14,15] (Scheme 1) are oxidized on carbon electrodes in aqueous solutions and all present a pH-dependent electron transfer mechanism [12].…”

Section: Introductionmentioning

confidence: 99%

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Direct Electrochemistry of Native and Denatured Anticancer Antibody Rituximab at a Glassy Carbon Electrode

2013

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Rituximab (RTX) is a human/murine chimeric monoclonal antibody (mAb) that specifically targets the transmembrane protein CD20 of B-cells. The oxidation mechanism of native and denatured RTX was investigated on glassy carbon electrode. The denaturing agent sodium dodecyl sulfate and the redutancts tris(2-carboxyethyl)phosphine and dithiothreitol were used. Significant differences were observed for native and denatured RTX oxidation due to morphological changes and unfolding of the RTX native structure. Native RTX presented only one oxidation peak of tyrosine and tryptophan residues, whereas in denatured RTX were detected three peaks corresponding to the oxidation of tyrosine, tryptophan and histidine residues.

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Boron doped diamond and glassy carbon electrodes comparative study of the oxidation behaviour of cysteine and methionine

Cited by 142 publications

References 23 publications

Glutaraldehyde Cross-Linked Glutamate Oxidase Coated Microelectrode Arrays: Selectivity and Resting Levels of Glutamate in the CNS

Glutaraldehyde Cross-Linked Glutamate Oxidase Coated Microelectrode Arrays: Selectivity and Resting Levels of Glutamate in the CNS

Poly-l-lysine-modified boron-doped diamond electrodes for the amperometric detection of nucleic acid bases

Direct Electrochemistry of Native and Denatured Anticancer Antibody Rituximab at a Glassy Carbon Electrode

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