Both bovine and rabbit lymphocytes conditioned with hydrogen peroxide show an adaptive response to radiation damage

Flores, María José; Piñero, J.; Ortiz, T.; Pastor, Nuria; Mateos, J.C.; Cortés, Felipe

doi:10.1016/s0027-5107(96)00082-6

Cited by 23 publications

(14 citation statements)

References 40 publications

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“…Although the results presented here are from an in vitro study in a piscine species, the nature of the findings was similar to those reported from earlier in vivo studies by Boreham and Mitchell [1993], Schappi-Buchi [1994], Broome et al [2002], Flores et al [1996], Azzam et al [1994], and by Wolff [1996] at the chromosomal and cellular levels, and also from the in vitro studies with ungulate species cells [Ulsh et al, 2004]. The significant difference being the doses required to prime and challenge were an order of magnitude higher and the time required for the AR to become apparent was at least 48 hr greater than that for the other biological systems.…”

Section: Discussionsupporting

confidence: 89%

“…AR has been observed in organisms ranging from yeast and other single-celled organisms [Boreham and Mitchel, 1993], insects [Schappi-Buchi, 1994], to humans [Broome et al, 2002], rabbits and cows [Flores et al, 1996], and laboratory rodents [Azzam et al, 1994;Wolff, 1996] at chromosomal and cellular levels. Early research with a variety of organisms [Luckey, 1980], and more recently with mice demonstrate that this is also true at the organismal level, although how this might translate to the population, community, or higher levels of biological organization is less clear.…”

Section: Introductionmentioning

confidence: 99%

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Characteristics of the adaptive response in cultured salmon cells exposed to ionizing radiation

Kilemade

Lemon

Boreham

2007

Environ and Mol Mutagen

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The aim of this study was to investigate the influence of "priming" doses of ionizing irradiation on salmon cell survival in vitro prior to being challenged with subsequent higher doses. A radiation-induced adaptive response (AR) was examined in the Chinook salmon embryo cell line (CHSE-214). Cells were initially irradiated with a range of priming (conditioning) doses of (60)Co gamma (gamma) rays (0.25-0.75 Gy), followed by a challenge dose of 7.50 Gy at intervals of 24, 48, and 72 hr. The AR was assessed using a colony-forming assay. Cell survival was determined by counting the number of colonies (viable clones) after 40 days of culture. This study revealed that cells that received a priming dose of 0.50 Gy before delivering the higher challenge dose became more radiation resistant with an increase in cell survival of 29% over cells receiving the challenge dose alone. The cells showed maximum resistance to ionizing radiation when the priming dose was given 72 hr prior to the higher challenge dose. This study is one of the first to demonstrate an AR using an in vitro piscine system, and is generally consistent with other studies of both in vitro and in vivo systems across the taxa.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Characteristics of the adaptive response in cultured salmon cells exposed to ionizing radiation

Kilemade

Lemon

Boreham

2007

Environ and Mol Mutagen

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“…It has become a tool to investigate and evaluate damage caused by environmental pollution on man and other animals (Hooftman 1981, Heddle et al 1983, Majone et al 1990. It is adopted in humans to evaluate the mutagenic effect of drugs before they are commercialized (Heddle et al 1991), and it is also used to estimate chromosome damage as a consequence of radiation (Fenech and Morley 1985, Kormos and Koteles 1988, Wuttke et al 1993, Flores et al 1996, Kim et al 1997). The study of micronuclei in buffalo by cytokinesis block method has demonstrated that , compared to other cytogenetic methods for evalution of chromosomal stability, the MN assay is relatively simple and rapid for scoring of large number of cells.…”

Section: Discussionmentioning

confidence: 99%

“…Cytokinesis-block (CB) method is used as a suitable method for scoring quantitation micronuclei in lymphocytes of human, mice, goat, rabbit and cattle (Fenech and Morly 1985, Scarfi et al 1993, Flores et al 1996, Kim et al 1997. It is based on the identification of the divided nuclei by blocking cytoplasmic division using cytochalasin-B (cyt-B).…”

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confidence: 99%

Measurement of Micronuclei by Cytokinesis-Block Method in Buffalo Lymphocytes.

Ahmed¹,

El-Miniawey

2000

CYTOLOGIA

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“…It is an example of a more general stress response [1]. For example, cross-adaptation has been observed between exposure to radiation and metals [2], chemicals [3,4] and hyperthermia [5,6].…”

Section: Introductionmentioning

confidence: 99%

Bystander effects in bullfrog tadpoles

Audette-Stuart

Yankovich

2011

Radioprotection

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Abstract. Adaptive responses were observed using the micronucleus frequency in bullfrog tadpoles. In tanks in which control tadpoles were placed in contact with tadpoles that were previously housed in tritiated water (3.0 × 10 4 Bq/L), the cells from all animals responded as though they were "adapted". This suggests that direct exposure to 3.0 × 10 4 Bq/L tritium contributes to an increased resistance to a high dose of radiation in liver cells. It also suggests that being in contact with tadpoles that were previously exposed to 3.0 × 10 4 Bq/L tritium (bystander effect) contributes to an increased resistance to a high dose of radiation in liver cells. In vitro exposures were also conducted using primary cultures of liver cells obtained from an unexposednon-bystander tadpole. In these control cells, it was observed that exposure to 100 mGy of 60 Co gamma radiation (delivered at a dose rate of 5 mGy/min) did not affect the micronucleus frequency whereas exposure to 4 Gy (delivered at a dose rate of about 10.2 Gy/min) increased the micronucleus frequency. Prior exposure to a low dose of 60 Co gamma radiation (100 mGy delivered at a dose rate of 5 mGy/min) induced an adaptive response, protecting the cells from harm caused by exposure to subsequent high doses of 60 Co gamma radiation (4 Gy delivered at a dose rate of about 10.2 Gy/min). Using the adaptive response (determined using micronucleus assay) as a biomarker, the data obtained suggest that bystander effects do play a role in wild populations since bullfrog tadpoles that were not exposed to tritium responded like the tadpoles that were directly exposed to tritium after being placed in contact with them.

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Both bovine and rabbit lymphocytes conditioned with hydrogen peroxide show an adaptive response to radiation damage

Cited by 23 publications

References 40 publications

Characteristics of the adaptive response in cultured salmon cells exposed to ionizing radiation

Characteristics of the adaptive response in cultured salmon cells exposed to ionizing radiation

Measurement of Micronuclei by Cytokinesis-Block Method in Buffalo Lymphocytes.

Bystander effects in bullfrog tadpoles

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