Both overlapping and independent loci underlie seed number per pod and seed weight in Brassica napus by comparative quantitative trait loci analysis

Ovule number (ON) produced during ower development determines the maximum number of seeds per silique and thereby affects crop productivity; however, the genetic control of ON remains poorly understood in oilseed rape (Brassica napus). In this study, we genetically dissected the ON variations in a double haploid population (DH) and in diverse inbred accessions by linkage mapping and genome-wide association analysis. Phenotypic analysis in six environments showed that ON displayed normal distribution in both populations with the broad-sense heritability of 0.861 (DH population) and 0.930 (natural population). Linkage mapping identi ed 5 QTLs related to ON, including qON-A03, qON-A07, qON-A07-2, qON-A10, and qON-C06. Genome-wide association studies (GWAS) revealed 214, 48 and 40 signi cant single nucleotide polymorphisms (SNPs) by individually using the single-locus model GLM and the multiple-locus model MrMLM and FASTMrMLM. The phenotypic variation explained (PVE) by these QTLs and SNPs ranged from 2.00-17.40% and 5.03-7.33%, respectively. Integration of the results from both strategies identi ed four consensus genomic regions associated with ON from the Chr A03, A07, and A10. Our results preliminarily resolved the genetic basis of ON and provides useful molecular markers for plant yield improvement in B. napus.

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Identification of a candidate QTG for seed number per silique by integrating QTL mapping and RNA-seq in Brassica napus L.

Xin

Dong²,

Cui³

et al. 2023

The Crop Journal

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Both overlapping and independent loci underlie seed number per pod and seed weight in Brassica napus by comparative quantitative trait loci analysis

Cited by 7 publications

References 61 publications

High-Throughput Association Mapping in Brassica napus L.: Methods and Applications

High-Throughput Association Mapping in Brassica napus L.: Methods and Applications

Linkage and association mapping of ovule number per ovary (ON) in oilseed rape (Brassica napus L.)

Identification of a candidate QTG for seed number per silique by integrating QTL mapping and RNA-seq in Brassica napus L.

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