Bottom-illuminated orbital shaker for microalgae cultivation

Nedbal, Jakub; Gao, Lu; Suhling, Klaus

doi:10.1016/j.ohx.2020.e00143

Cited by 15 publications

(12 citation statements)

References 48 publications

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“…These devices mainly vary with respect to the culture volumes they hold (from 1 to 50 mL); in their geometry, which impacts shaking and illumination; and in their illumination modalities, which impacts cells exposure to light. Each optogenetic device corresponds to a standard lab-scale culture device, and alternative designs exist for each device and purpose: screen straining in imaging plates like the OptoBox 18,[41][42][43] , illumination of starter cultures in OptoTubes 44 , monitoring multiple culture parameters in minibioreactors such as eVOLVER 19,45,46 , or use of larger culture volumes for various purposes in OptoFlasks 47 . Measuring the intensities of the LEDs allows us to compare the relative amounts of input light across these devices.…”

Section: Discussionmentioning

confidence: 99%

Optogenetic control of beta-carotene bioproduction in yeast across multiple lab-scales

Pouzet

Cruz-Ramon

Bec

et al. 2022

Preprint

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Optogenetics arises as a valuable tool to precisely control genetic circuits in microbial cell factories. Light control holds the promise of optimizing bioproduction methods and maximize yields, but its implementation at different steps of the strain development process and at different culture scales remains challenging. In this study, we aim to control beta-carotene bioproduction using optogenetics in Saccharomyces cerevisiae and investigate how its performance translates across culture scales. We built four lab-scale illumination devices, each handling different culture volumes, and each having specific illumination characteristics and cultivating conditions. We evaluated optogenetic activation and beta-carotene production across devices and optimized them both independently. Then, we combined optogenetic induction and beta-carotene production to make a light-inducible beta-carotene producer strain. This was achieved by placing the transcription of the bifunctional lycopene cyclase / phytoene synthase CrtYB under the control of the pC120 optogenetic promoter regulated by the EL222-VP16 light-activated transcription factor, while other carotenogenic enzymes (CrtI, CrtE, tHMG) were expressed constitutively. We show that illumination, culture volume and shaking impact differently optogenetic activation and beta-carotene production across devices. This enabled us to determine the best culture conditions to maximize light-induced beta-carotene production in each of the devices, reaching a content of up to 880 micro-g/gCDW. Our study exemplifies the stakes of scaling up optogenetics in devices of different lab scales and sheds light on the interplays and potential conflicts between optogenetic control and metabolic pathway efficiency. As a general principle, we propose that it is important to first optimize both components of the system independently, before combining them into optogenetic producing strains to avoid extensive troubleshooting. We anticipate that our results can help designing both strains and devices that could eventually lead to larger scale systems in an effort to bring optogenetics to the industrial scale.

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Section: Discussionmentioning

confidence: 99%

Optogenetic control of beta-carotene bioproduction in yeast across multiple lab-scales

Pouzet

Cruz-Ramon

Bec

et al. 2022

Preprint

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“…The relative intensity of the different color LEDs is easy to control via programming. The carbon source for photoautotrophic microorganisms is CO 2 and it can be provided by shaking, stirring or via gas sparging to ensure microalgae cell mixing, and nutrient and gas exchange [ 10 ].…”

Section: Hardware In Contextmentioning

confidence: 99%

“…Indoor microalgae cultivation is usually done in climate-controlled growth rooms/cabinets where an orbital shaker is typically used for agitation. Small scale LED systems for microalgae cultivation mounted on orbital shakers are seldom published [ [10] , [14] ] while publications regarding microalgae cultivation under different LED source light regimes are more common [ [15] , [16] , [17] , [18] , [19] ].…”

Section: Hardware In Contextmentioning

confidence: 99%

CultureLED: A 3D printer-based LED illumination cultivation system for multi-well culture plates

Hasson

Wishkerman

2022

HardwareX

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“…The correlation appears weaker when compared to the scatterplot charts for sunlight and overcast days. This suggests that the spectral composition of the artificial light sources are not the same as sunlight and thus the one set of coefficients calibrated from the sun will not be as accurate when used in a different setting as suggested by Nedbal et al [29]. These points indicate that an inexpensive IoT sensor, calibrated using only sunlight, will struggle to accurately measure PAR light from a variety of sources.…”

Section: Introductionmentioning

confidence: 96%

Adaptalight: An Inexpensive PAR Sensor System for Daylight Harvesting in a Micro Indoor Smart Hydroponic System

et al. 2022

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Environmental changes and the reduction in arable land have led to food security concerns around the world, particularly in urban settings. Hydroponic soilless growing methods deliver plant nutrients using water, conserving resources and can be constructed nearly anywhere. Hydroponic systems have several complex attributes that need to be managed, and this can be daunting for the layperson. Micro Indoor Smart Hydroponics (MISH) leverage Internet of Things (IoT) technology to manage the complexities of hydroponic techniques, for growing food at home for everyday citizens. Two prohibitive costs in the advancement of MISH systems are power consumption and equipment expense. Reducing cost through harvesting ambient light can potentially reduce power consumption but must be done accurately to sustain sufficient plant yields. Photosynthetic Active Radiation (PAR) meters are commercially used to measure only the light spectrum that plants use, but are expensive. This study presents Adaptalight, a MISH system that harvests ambient light using an inexpensive AS7265x IoT sensor to measure PAR. The system is built on commonly found IoT technology and a well-established architecture for MISH systems. Adpatalight was deployed in a real-world application in the living space of an apartment and experiments were carried out accordingly. A two-phase experiment was conducted over three months, each phase lasting 21 days. Phase one measured the IoT sensor’s capability to accurately measure PAR. Phase two measured the ability of the system to harvest ambient PAR light and produce sufficient yields, using the calibrated IoT sensor from phase one. The results showed that the Adaptalight system was successful in saving a significant amount of power, harvesting ambient PAR light and producing yields with no significant differences from the control. The amount of power savings would be potentially greater in a location with more ambient light. Additionally, the findings show that, when calibrated, the AS7265x sensor is well suited to accurately measure PAR light in MISH systems.

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Bottom-illuminated orbital shaker for microalgae cultivation

Cited by 15 publications

References 48 publications

Optogenetic control of beta-carotene bioproduction in yeast across multiple lab-scales

Optogenetic control of beta-carotene bioproduction in yeast across multiple lab-scales

CultureLED: A 3D printer-based LED illumination cultivation system for multi-well culture plates

Adaptalight: An Inexpensive PAR Sensor System for Daylight Harvesting in a Micro Indoor Smart Hydroponic System

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