Bottom up effects on bacterioplankton growth and composition during summer-autumn transition in the open NW Mediterranean Sea

Wambeke, F. Van; Ghiglione, Jean-François; Nedoma, Jiří; Mével, Geneviève; Raimbault, Patrick

doi:10.5194/bg-6-705-2009

Cited by 41 publications

(38 citation statements)

References 60 publications

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“…The outcompeting cells developing in such conditions are organisms with an "r-strategy", as demonstrated in our experiments with HNA+ cells. This could explain the classically reported loss or shift in diversity found in other amendment experiments (Eilers et al, 2000;Schafer et al, 2000;Massana et al, 2001;Van Wambeke et al, 2009). …”

Section: Effect Of Nutrient Addition On the Hprok Leucine Incorporatimentioning

confidence: 72%

“…Indeed, differences between surface and DCM layers, in terms of habitat for Hprok, are noticeable under stratified conditions. With changing limiting factors (switch from N-P limitation to C limitation: Sala et al, 2002;Van Wambeke et al, 2002) and changes in phytoplankton populations which probably fuel different carbon resources along the water column, the result is a change in the Hprok taxonomic composition (Ghiglione et al, 2008). …”

Section: Large Variability Of Hna Leucine Incorporation Ratesmentioning

confidence: 99%

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Flow cytometric assessment of specific leucine incorporation in the open Mediterranean

et al. 2011

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Abstract. The surface of the Mediterranean Sea is a lowphosphate-low-chlorophyll marine area where marine heterotrophic prokaryotes significantly contribute to the biogeochemical cycles of all biogenic elements such as carbon, notably through the mineralization of dissolved organic compounds. Cell-specific leucine incorporation rates were determined in early summer in the open stratified Mediterranean Sea. The bulk leucine incorporation rate was on average 5 ± 4 pmol leu l −1 h −1 (n = 30). Cell-specific 3 Hleucine incorporation rates were assayed using flow cytometry coupled to cell sorting. Heterotrophic prokaryotes (Hprok) were divided into cytometric groups according to their side scatter and green fluorescence properties: high nucleic acid containing cells (HNA) with high scatter (HNAhs) and low scatter (HNA-ls) and low nucleic acid containing cells (LNA). Cell-specific leucine incorporation rates of these cytometric groups ranged from 2 to 54, 0.9 to 11, and 1 to 12 × 10 −21 mol cell −1 h −1 , respectively. LNA cells represented 45 to 63% of the Hprok abundance, and significantly contributed to the bulk leucine incorporation rates, from 12 to 43%. HNA/LNA ratios of cell-specific leucine incorporation were on average 2.0 ± 0.7 (n = 30). In surface layers (from 0 m down to the deep chlorophyll depth, DCM), cell-specific rates of HNA-hs were elevated (7 and 13 times greater than LNA and HNA-ls, respectively). Nevertheless, on average HNA-hs (26%) and LNA (27%) equally contributed to the bulk leucine incorporation in these layers. Prochlorococcus cells were easily sorted near the DCM and displayed cell-specific leucine incorporation rates ranging from 3 to 55 × 10 −21 mol leu cell −1 h −1 , i.e. as high asCorrespondence to: A. Talarmin (agathe.talarmin@univmed.fr) HNA-hs'. These sorted groups could therefore be defined as key-players in the process of leucine incorporation into proteins. The mixotrophic features of certain photosynthetic prokaryotes and the high contribution of LNA cells to leucine incorporation within the microbial communities of the Mediterranean could be reinforced.

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Section: Effect Of Nutrient Addition On the Hprok Leucine Incorporatimentioning

confidence: 72%

Section: Large Variability Of Hna Leucine Incorporation Ratesmentioning

confidence: 99%

Flow cytometric assessment of specific leucine incorporation in the open Mediterranean

et al. 2011

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“…Possible explanations for these trends are a switch from nutrient to carbon limitation which is generally observed within the dcm in stratified conditions (Sala et al, 2002;Van Wambeke et al, 2002). This is associated with large changes in taxonomic composition of heterotrophic prokaryotes Van Wambeke et al, 2009). Apparently the HNA group benefits more of such changes than the LNA.…”

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confidence: 99%

Vertical and longitudinal gradients in HNA-LNA cell abundances and cytometric characteristics in the Mediterranean Sea

et al. 2011

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Abstract. Heterotrophic bacterioplankton abundance and production were investigated with depth (down to bathypelagic layers) and with longitude (from 4.9 • E to 32.7 • E) along a cruise track across the Mediterranean Sea in early summer 2008. Abundances and flow cytometric characteristics (green fluorescence and side scatter signals) of high nucleic acid (HNA) and low nucleic acid (LNA) bacterial cells were determined using flow cytometry. Contrary to what is generally observed, the relative importance of HNA cells, as a percent of total cells, (%HNA, range 30-69 %) was inversely related to bacterial production (range 0.15-44 ng C l −1 h −1 ) although the negative relation was weak (log-log regression r 2 = 0.19). The %HNA as well as the mean side scatter of HNA group increased significantly with depth in the meso and bathypelagic layers. Vertical stratification played an important role in influencing the distribution and characteristics of bacterial cells especially with regard to layers located above, within or below the deep chlorophyll maximum. Within a given layer, the relationships between the flow cytometric characteristics and environmental variables such as chlorophyll-a, nutrients or bacterial production changed. Overall, the relationships between HNA and LNA cells and environmental parameters differed vertically more than longitudinally.

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“…Sediments are generally considered to be a significant internal source of nutrients in shallow coastal ecosystems, but they may also be a net sink of dissolved nitrogen, at least during certain times of the year (Sundbäck et al, 2000;Tyler et al, 2003). Rivera-Monroy et al (1995a) measured nitrogen fluxes between Estero Pargo (an unpolluted tidal creek), and a fringe mangrove forest in the Términos Lagoon.…”

Section: Biogeochemical Characteristics Of the Términosmentioning

confidence: 99%

“…These saturated concentrations and optimized time incubations were determined prior to the extracellular enzymatic activities measurement as previously described by Van Wambeke et al, 2009.…”

Section: Extracellular Enzymatic Activitiesmentioning

confidence: 99%

Biogeochemical cycling and phyto- and bacterioplankton communities in a large and shallow tropical lagoon (Términos Lagoon, Mexico) under 2009–2010 El Niño Modoki drought conditions

et al. 2017

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Abstract. The 2009-2010 period was marked by an episode of intense drought known as the El Niño Modoki event. Sampling of the Términos Lagoon (Mexico) was carried out in November 2009 in order to understand the influence of these particular environmental conditions on organic matter fluxes within the lagoon's pelagic ecosystem and, more specifically, on the relationship between phyto-and bacterioplankton communities. The measurements presented here concern biogeochemical parameters (nutrients, dissolved and particulate organic matter [POM], and dissolved polycyclic aromatic hydrocarbons [PAHs]), phytoplankton (biomass and photosynthesis), and bacteria (diversity and abundance, including PAH degradation bacteria and ectoenzymatic activities). During the studied period, the water column of the Términos Lagoon functioned globally as a sink and, more precisely, as a "nitrogen assimilator". This was due to the high production of particulate and dissolved organic matter (DOM), even though exportation of autochthonous matter to the Gulf of Mexico was weak. We found that "bottom-up" control accounted for a large portion of the variability of phytoplankton productivity. Nitrogen and phosphorus stoichiometry mostly accounted for the heterogeneity in phytoplankton and free-living prokaryote distribution in the lagoon. In the eastern part, we found a clear decoupling between areas enriched in dissolved inorganic nitrogen near the Puerto Real coastal inlet and areas enriched in phosphate (PO 4 ) near the Candelaria estuary. Such a decoupling limited the potential for primary production, resulting in an accumulation of dissolved organic carbon and nitrogen (DOC and DON, respectively) near the river mouths. In the western part of the lagoon, maximal phytoplankton development resulted from bacterial activity transforming particulate organic phosphorus (PP) and dissolved organic phosphorus (DOP) to available PO 4 and the coupling between Palizada River inputs of nitrate (NO 3 ) and PP. The Chumpan River contributed only marginally to PO 4 inputs due to its very low contribution to overall river inputs. The highest dissolved total PAH concentrations were measured in the El Carmen Inlet, suggesting that the anthropogenic pollution of the zone is probably related to the oil-platform exploitation activities in the shallow waters of the southern of the Gulf of Mexico. We also found that a complex array of biogeochemical and phytoplanktonic parameters were the driving force behind the geographical distribution of bacterial community structure and activities. Finally, we showed that nutrients brought by the Palizada River supported an abundant bacterial community of PAH

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Bottom up effects on bacterioplankton growth and composition during summer-autumn transition in the open NW Mediterranean Sea

Cited by 41 publications

References 60 publications

Flow cytometric assessment of specific leucine incorporation in the open Mediterranean

Flow cytometric assessment of specific leucine incorporation in the open Mediterranean

Vertical and longitudinal gradients in HNA-LNA cell abundances and cytometric characteristics in the Mediterranean Sea

Biogeochemical cycling and phyto- and bacterioplankton communities in a large and shallow tropical lagoon (Términos Lagoon, Mexico) under 2009–2010 El Niño Modoki drought conditions

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