Boule gene expression underpins the meiotic arrest in spermatogenesis in male rainbow trout ( Oncorhynchus mykiss ) exposed to DEHP and butachlor

Ahmadivand, Sohrab; Farahmand, Hamid; Teimoori‐Toolabi, Ladan; Mirvaghefi, Alireza; Eagderi, Soheil; Geerinckx, Tom; Shokrpoor, Sara; Rahmati‐Holasoo, Hooman

doi:10.1016/j.ygcen.2015.05.011

Cited by 9 publications

(5 citation statements)

References 67 publications

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“…BOLL ( Boule ), an ancestral gene of the DAZ family, is evolutionarily conserved in all metazoans [5,12]. Numerous studies have shown that the BOLL gene plays an important role in testicular function, maintenance, and spermatogenesis and that loss of the gene may cause male dyszoospermia and infertility [9,13,14,15,16]. In females, BOLL may regulate human embryonic stem cells to enter into meiotic phase and produce meiotic germ cells which are further induced to form ovarian follicle-like cells by expressing DAZL and BOLL with recombinant GDF9 and BMP15 [17].…”

Section: Introductionmentioning

confidence: 99%

Histomorphological Comparisons and Expression Patterns of BOLL Gene in Sheep Testes at Different Development Stages

Wang

Zhang

et al. 2019

Animals

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BOLL is implicated in mammalian testicular function maintenance and spermatogenesis. To understand the expression patterns and biological functions of sheep BOLL, we examined the expression and immunolocalization of BOLL in the developing testes of Small-Tail Han sheep aged 0 days (D0), 2 months (2M), 5 months (5M), 1 year (1Y), and 2 years (2Y), by qPCR, Western blot, and immunohistochemistry methods. Firstly, morphological studies revealed that, in addition to spermatogonia, ordered and clear spermatocytes, as well as round and elongated spermatids and sperm, were found in the 1Y and 2Y testicular seminiferous tubules of the sheep testes, compared with the D0, 2M, and 5M testes, as analyzed by hematoxylin and eosin (H&E) staining. The diameter and area of the seminiferous tubules, epithelial thickness, and the area and perimeter of the tubule lumens gradually increased with age. BOLL was specifically expressed in testes and upregulation of BOLL transcript expression was higher in the testes of the 1Y and 2Y groups than in those of the D0, 2M, and 5M groups. Similarly, BOLL protein was expressed mainly in the 1Y and 2Y testes, ranging from primary spermatocytes to round spermatids, as well as in the spermatozoa. This study is the first demonstration that sheep BOLL might serve as a key regulator of the spermiogenesis involved in sperm maturity, in addition to its role as a crucial meiotic regulator.

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Section: Introductionmentioning

confidence: 99%

Histomorphological Comparisons and Expression Patterns of BOLL Gene in Sheep Testes at Different Development Stages

Wang

Zhang

et al. 2019

Animals

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“…Interestingly, the human BOLL ( BOULE ) gene can rescue reproductive defects in Drosophila , 24 which supports the idea that BOLL may encode a key conserved switch that regulates the progression of germ cells through meiosis in men. The mechanism by which BOLL participates in meiosis may be that it regulates CDC25A translation, and CDC25A participates in mitosis or meiosis by regulating the transition of the G1/S phase and exit of the M phase 25 . BOLL gene expression levels in the testis have been demonstrated to be an indicator of meiosis efficiency in rainbow trout 25 and infertile men, 23 as well as the presence of sperm in the testis 26 .…”

Section: Discussionmentioning

confidence: 99%

“…The mechanism by which BOLL participates in meiosis may be that it regulates CDC25A translation, and CDC25A participates in mitosis or meiosis by regulating the transition of the G1/S phase and exit of the M phase 25 . BOLL gene expression levels in the testis have been demonstrated to be an indicator of meiosis efficiency in rainbow trout 25 and infertile men, 23 as well as the presence of sperm in the testis 26 . In the present study, we demonstrated that this gene's cfs‐mRNA was predominately derived from the testis and germ cells, since the levels of cfs‐mRNAs in both SCOS and OA patients were undetectable or very low.…”

Section: Discussionmentioning

confidence: 99%

Multi‐stage screening cell‐free seminal mRNAs to diagnose completion of meiosis and predict testicular sperm retrieval in men with non‐obstructive azoospermia

et al. 2022

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Background: Differential diagnosis of men with subtypes of non-obstructive azoospermia (NOA) is important for their treatment. Many genes are transcripted during meiosis. We hypothesized that some of these genes can be detected in cell-free seminal message RNA (mRNAs) (cfs-mRNA) and be developed as non-invasive biomarkers for diagnosing NOA subtypes.Objective: To screen cfs-mRNA to diagnose the completion of meiosis and predict successful sperm retrieval (SR) in men with NOA. Materials and methods: NOA patients who visited our institutes from September 2018 to December 2020 for testicular histopathological diagnosis (n = 109) or testicular SR (n = 92) were screened for participation in the study. Microarray and real-time quantitative polymerase chain reaction were used in five stages to obtain candidate cfs-mRNAs for comparisons between patients with early maturation arrest (eMA, meiosis not completed) and late MA or hypospermatogenesis (meiosis completed) and between NOA patients with successful SR and SR failure.Results: Twelve cfs-mRNAs were selected based on this comparison between men with eMA and hypospermatogenesis and their gene expression and function information. Of these, AKAP1, BOLL, TCP11 and SETX predominantly derived from testes and germ cells were proposed as candidate cfs-mRNAs. Further quantification in men with NOA demonstrated significantly higher levels of BOLL cfs-mRNA (p < 0.0001) in men with late MA or hypospermatogenesis (n = 23), compared with men with eMA (n = 51); and significantly higher levels (p < 0.0001) in patients with successful SR (n = 44) when compared with patients with SR failure (n = 37). Interestingly, with a similar cutoff value, BOLL cfs-mRNA showed high sensitivity and specificity in diagnosing late MA and hypospermatogenesis (>404 copies/ml) and predicting successful SR

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“…Total RNA was then extracted from the cells using the ReliaPrep™ RNA Cell Miniprep System (Promega), and cDNA was synthesized using the GoScript™ Reverse Transcription System Kit (Promega). The qPCR was performed using Fast SYBR™ Green Master Mix (ThermoFisher, DE) ( 32 ),, for gene markers of the innate immune response to viral infection ( mx, vig1, ifit5 , and isg-15 ) ( 33 , 34 ),. The expression of the target genes has been corrected based on a reference gene ( EF-1 α ) and calculated relative to the control via the 2 - ΔΔCt method ( 35 ).…”

Section: Methodsmentioning

confidence: 99%

Self-assembling ferritin nanoplatform for the development of infectious hematopoietic necrosis virus vaccine

Ahmadivand,

Krpetic,

Martínez

et al. 2024

Front. Immunol.

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Self-assembling protein nanoparticles are used as a novel vaccine design platform to improve the stability and immunogenicity of safe subunit vaccines, while providing broader protection against viral infections. Infectious Hematopoietic Necrosis virus (IHNV) is the causative agent of the WOAH-listed IHN diseases for which there are currently no therapeutic treatments and no globally available commercial vaccine. In this study, by genetically fusing the virus glycoprotein to the H. pylori ferritin as a scaffold, we constructed a self-assembling IHNV nanovaccine (FerritVac). Despite the introduction of an exogenous fragment, the FerritVac NPs show excellent stability same as Ferritin NPs under different storage, pH, and temperature conditions, mimicking the harsh gastrointestinal condition of the virus main host (trout). MTT viability assays showed no cytotoxicity of FerritVac or Ferritin NPs in zebrafish cell culture (ZFL cells) incubated with different doses of up to 100 µg/mL for 14 hours. FerritVac NPs also upregulated expression of innate antiviral immunity, IHNV, and other fish rhabdovirus infection gene markers (mx, vig1, ifit5, and isg-15) in the macrophage cells of the host. In this study, we demonstrate the development of a soluble recombinant glycoprotein of IHNV in the E. coli system using the ferritin self-assembling nanoplatform, as a biocompatible, stable, and effective foundation to rescue and produce soluble protein and enable oral administration and antiviral induction for development of a complete IHNV vaccine. This self-assembling protein nanocages as novel vaccine approach offers significant commercial potential for non-mammalian and enveloped viruses.

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Boule gene expression underpins the meiotic arrest in spermatogenesis in male rainbow trout ( Oncorhynchus mykiss ) exposed to DEHP and butachlor

Cited by 9 publications

References 67 publications

Histomorphological Comparisons and Expression Patterns of BOLL Gene in Sheep Testes at Different Development Stages

Histomorphological Comparisons and Expression Patterns of BOLL Gene in Sheep Testes at Different Development Stages

Multi‐stage screening cell‐free seminal mRNAs to diagnose completion of meiosis and predict testicular sperm retrieval in men with non‐obstructive azoospermia

Self-assembling ferritin nanoplatform for the development of infectious hematopoietic necrosis virus vaccine

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