Bovine chondrocyte behaviour in three-dimensional type I collagen gel in terms of gel contraction, proliferation and gene expression

Galois, Laurent; Hutasse, S.; Cortial, Delphine; Rousseau, Cécile F.; Grossin, Laurent; Ronzière, Marie‐Claire; Herbage, D.; Freyria, Anne‐Marie

doi:10.1016/j.biomaterials.2005.05.098

Cited by 104 publications

(76 citation statements)

References 43 publications

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“…In all tested scaffolds, transcript levels of cartilage-related genes, like Col 2, Agr, Sox 9, were upregulated. [27][28][29][30][31][32] Two different types of hydrogels were examined in the present study: alginate and alginate/agarose. A 14-day incubation period was selected to allow for sufficient redifferentiation.…”

Section: Human Chondrocyte Gene Expression In Vitromentioning

confidence: 99%

Quantitative analysis of gene expression in human articular chondrocytes assigned for autologous implantation

Barlič

Drobnič

Maličev

et al. 2008

Journal Orthopaedic Research

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Autologous chondrocyte implantation (ACI) relies on the implantation of in vitro expanded cells. The aim was to study the dedifferentiation of human articular chondrocytes under different cultivating conditions [days 0-10 in the primary culture (P0); passages in a monolayer from P0 to P3; monolayer vs. alginate and monolayer vs. alginate/agarose hydrogels] using real-time PCR analysis. The relative gene expressions for collagen type I and II, aggrecan and versican were quantified and the corresponding differentiation indexes (Col2/Col1, Agr/Ver) were calculated. The values of both differentiation indexes decreased exponentially with time in the P0 monolayer culture, and continued with a significant decrease over the subsequent monolayer passages. On the contrary, the chondrocytes seeded in either of the hydrogels significantly increased the indexes compared to their parallel monolayer cultures. These results indicate that alginate and alginate/agarose hydrogels offer an appropriate environment for human articular chondrocytes to redifferentiate after being expanded in vitro. Therefore the three-dimensional (3D) hydrogel chondrocyte cultures present not only surgical, but also biological advantage over the classic suspension-periosteum chondrocyte implantation. ß

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Section: Human Chondrocyte Gene Expression In Vitromentioning

confidence: 99%

Quantitative analysis of gene expression in human articular chondrocytes assigned for autologous implantation

Barlič

Drobnič

Maličev

et al. 2008

Journal Orthopaedic Research

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“…Conversely, chondrocytes don't segregate in collagen type-I gels (Galois et al, 2006). Thus, in analogy to cell-cell sorting (Steinberg, 1970), not all combinations of cells and ECM lead to spatial segregation of cells.…”

Section: Tissue Architecture In Stromal Microtissuesmentioning

confidence: 99%

3D culture models of tissues under tension

Eyckmans

Chen

2016

Journal of Cell Science

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Cells dynamically assemble and organize into complex tissues during development, and the resulting three-dimensional (3D) arrangement of cells and their surrounding extracellular matrix in turn feeds back to regulate cell and tissue function. Recent advances in engineered cultures of cells to model 3D tissues or organoids have begun to capture this dynamic reciprocity between form and function. Here, we describe the underlying principles that have advanced the field, focusing in particular on recent progress in using mechanical constraints to recapitulate the structure and function of musculoskeletal tissues.

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“…15,16 Others include cell-mediated compaction or contraction of the scaffold, which may increase the mechanical strength of the material but typically are associated with more fibroblastic phenotypes. [34][35][36] Other approaches include functionalizing the scaffold or composing the scaffold wholly with the desired matrix proteins, 12,37,38 which increase the initial matrix concentration of the construct. In the present study, an 80% increase in COL concentration was achieved rapidly in a few days by a matrix assembly and mechanical dehydration method.…”

Section: Han Et Almentioning

confidence: 99%

Compaction Enhances Extracellular Matrix Content and Mechanical Properties of Tissue-Engineered Cartilaginous Constructs

Han

Ge²,

Chen³

et al. 2012

Tissue Engineering Part A

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Many cell-based tissue-engineered cartilaginous constructs are mechanically softer than native tissue and have low content and abnormal proportions of extracellular matrix (ECM) constituents. We hypothesized that the load-bearing mechanical properties of cartilaginous constructs improve with the inclusion of collagen (COL) and proteoglycan (PG) during assembly. The objectives of this work were to determine (1) the effect of addition of PG, COL, or COL + PG on compressive properties of 2% agarose constructs and (2) the ability of mechanical compaction to concentrate matrix content and improve the compressive properties of such constructs. The inclusion of COL + PG improved the compressive properties of hydrogel constructs compared with PG or COL alone. Mechanical compaction increased the PG and COL concentrations in and compressive stiffness of the constructs. Chondrocytes included in the constructs maintained high viability after compaction. These results support the concepts that the assembly of cartilaginous constructs with COL + PG and application of mechanical compaction enhance the ECM content and compressive properties of engineered cartilaginous constructs.

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Bovine chondrocyte behaviour in three-dimensional type I collagen gel in terms of gel contraction, proliferation and gene expression

Cited by 104 publications

References 43 publications

Quantitative analysis of gene expression in human articular chondrocytes assigned for autologous implantation

Quantitative analysis of gene expression in human articular chondrocytes assigned for autologous implantation

3D culture models of tissues under tension

Compaction Enhances Extracellular Matrix Content and Mechanical Properties of Tissue-Engineered Cartilaginous Constructs

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