Bovine herpesvirus 1 can impact the bovine oocyte development during in vitro maturation

Alves, Sabrina do Carmo; Costa, Eduardo Paulino da; Queiroz-Castro, Vanessa Lopes Dias; Machado‐Neves, Mariana; Guimarães, José Domingos; Gomes, Lidiane L.; Azevedo, Marcos Antônio; Júnior, Abelardo Silva

doi:10.1016/j.rvsc.2018.12.020

Cited by 8 publications

(6 citation statements)

References 37 publications

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“…In fact, several layers of this cell type surround the oocyte and form cumulus oocyte complexes (COCs), which play an essential role in maturation, fertilization and early embryo development processes [14,15]. Alves et al [16] reported that COCs obtained from cows naturally infected with BHV1 harbored virus-positive cumulus cells, and its presence impaired the in vitro nuclear maturation rate.…”

Section: Introductionmentioning

confidence: 99%

Bovine herpesvirus 1 can cross the intact zona pellucida of bovine oocytes after artificial infection

et al. 2019

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Bovine herpesvirus 1 (BHV1) is an important bovine pathogen, responsible for respiratory diseases and reproductive problems. This study investigated the penetration capacity of BHV1 into oocytes after co-incubation for either 1 h or 24 h. Immunofluorescence assays in cumulus -oocyte complexes (COCs) and denuded oocytes (without the presence of cumulus cells) were performed and evaluated using confocal laser scanning microscopy. Blood samples and ovaries from BHV1 seronegative cows were used. The oocytes recovered were divided into two groups. Group I comprised COCs (n = 312) and denuded oocytes (n = 296), which were experimentally infected with BHV1 and incubated for 1 h at 38.5°C and 5% CO 2 . Group II comprised COCs (n = 425) and denuded oocytes (n = 405), which were co-incubated with BHV1 under the same conditions for 24 h. The negative control of these two groups was respectively subjected to the same protocol, except for exposure to BHV1. To our knowledge, this study provides the first evidence of BHV1 detection within COCs and denuded oocytes exhibiting intact zona pellucida when co-incubated with the virus for 24 h. Immunolocalization also confirmed the presence of BHV1 in the cytoplasm of the cumulus cells of all COCs exposed to the virus after both incubation periods. In conclusion, detection of BHV1 inside oocytes has a great meaning for the field of animal reproduction. The detection of BHV1 in different layers of cumulus cells also demonstrates that these cells are sources of viral infection.

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Section: Introductionmentioning

confidence: 99%

Bovine herpesvirus 1 can cross the intact zona pellucida of bovine oocytes after artificial infection

et al. 2019

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“…2,7 There is a considerable information regarding the effect of BoHV-1 on breeding by both artificial insemination and natural service. 2,5,7,8 BoHV-1 infection can increase the frequency of sperm abnormalities such as sperm head decondensation. The pioneering work of Bielanski and Dubuc demonstrated that oocytes recovered from BoHV-1infected cows could mature and be fertilized in vitro, thus yielding transferable embryos.…”

Section: Transmission and Epidemiology Of Infectionmentioning

confidence: 99%

“…Subsequent studies have reported that BoHV-1 exerts negative effects on the in vitro embryo production system. 5,7,8 Different strains of BoHV-1, including a live attenuated vaccine, can remain in a latent state in clinically healthy bulls, only to be reactivated and excreted in their semen, thereby promoting transmission of infection to seronegative cattle. This requires restrictions on the use of semen from potentially infected bulls to produce embryos.…”

Section: Transmission and Epidemiology Of Infectionmentioning

confidence: 99%

“…4 It is worth emphasizing that oocytes containing BoHV-1 adhered to the ZP also allow for possible infection of the oviduct epithelial cells by the virus, due to its epitheliotropic nature, leading to cell lysis and thus poor embryo development. 5 The observed adherence of BoHV-1 to the ZP after the maturation period may enable its penetration into the oocyte at the moment of fertilization. 5 In addition, the virus may infect the recipient cow if a contaminated embryo is transferred.…”

Section: Transmission and Epidemiology Of Infectionmentioning

confidence: 99%

“…Any factor that triggers host immunosuppression may result in reactivation of BoHV-1 followed by episodes of virus re-excretion. [4][5][6]…”

Section: Introductionmentioning

confidence: 99%

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Bovine herpes virus-1: comparison of methods for removal of a commercially important pathogen from cattle sperm, oocytes and pre-implantation embryos

Robinson,

Sadeghzadeh

2019

JDVAR

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Bovine herpesvirus 1 (BoHV-1) is a pathogen of major veterinary importance, causing principally reproductive failure, genital and respiratory disease in cattle. Since embryo transfer is a rapidly growing commercial venture, BoHV-1 has a significant negative impact on cattle breeding by both natural and artificial service, and thereby on the global livestock industry. Clinical infection of the reproductive tract causes infertility, early embryonic death and abortion. BoHV-1 may infect an embryo by either of two means. The first is through entry of contaminated sperm into the oocyte at the point of fertilization, while the second is via contact with either contaminated follicular fluid, oviductal or uterine tissues. In addition, the virus may infect the recipient cow if an infected embryo is transferred by assisted reproduction technology. This article briefly examines the two principal methods that are routinely available to eliminate BoHV-1, performed in order to prevent infection of bovine embryos. Although each offers considerable benefits, it is also imperfect. Even after multiple trypsin washes BoHV-1 can adhere to the zona pellucida of oocytes and pre-implantation stage embryos; likewise, cryopreservation fails to eliminate all infectious virus particles. A more experimental technique, sperm processing, shows considerable promise but requires further validation as an effective way to remove BoHV-1 from bull semen before it can be recommended for industry-wide use.

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Impedimetric Biosensor for Bovine Herpesvirus Type 1‐Antigen Detection

et al. 2020

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The bovine herpesvirus type 1 (BHV‐1) is a pathogen of great economic impact for livestock, which is related multi‐systemic infections that leads to mortality or morbidity of cattles. Thus, the search for cheap and practical methodologies that allow the selective detection of BHV‐1 antigen (BHV‐1 AG) is of utmost relevance. Therefore, an impedimetric label‐free immunosensor was herein, developed and its performance evaluated in biological samples enriched with BHV‐1 AG. Briefly, the biosensor construction was based on the immobilization of BHV‐1 antibody (BHV‐1 AB) and casein on the activated glassy carbon electrode surface. The BHV‐1 AB was isolated from egg yolk of immunized chickens, which is a less stressful protocol. The bio sensing principle was based on Electrochemical Impedance Spectroscopy by using Fe(CN)64−/3− probe, which were also used to check variation of charge transfer resistance (▵Rct), when the electrode surface was increasingly blocked by immune complex. A linear relationship between ▵Rct and BHV‐1 AG concentration was verified in the range from 10 to 50 TCID50 mL−1, with LOQ of 2.00 TCID50 mL−1 and LOD of 0.66 TCID50 mL−1. Besides the suitable sensitivity, the immunosensor displayed accuracy, stability, and specificity to detect BHV‐1 AG in biological samples of serum, nasal secretions, semen and urine. Moreover, to the best of our knowledge this is the first immunosensor applied to BHV‐1 diagnostic.

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Bovine herpesvirus 1 can impact the bovine oocyte development during in vitro maturation

Cited by 8 publications

References 37 publications

Bovine herpesvirus 1 can cross the intact zona pellucida of bovine oocytes after artificial infection

Bovine herpesvirus 1 can cross the intact zona pellucida of bovine oocytes after artificial infection

Bovine herpes virus-1: comparison of methods for removal of a commercially important pathogen from cattle sperm, oocytes and pre-implantation embryos

Impedimetric Biosensor for Bovine Herpesvirus Type 1‐Antigen Detection

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