Bovine hydroxyapatite for bone tissue engineering: Preparation, characterization, challenges, and future perspectives

Adhikara, Arrival Gusti; Maharani, Agnesia Putri; Puspitasari, Anggie; Nuswantoro, Nuzul Ficky; Juliadmi, Dian; Maras, Muhammad Artha Jabatsudewa; Nugroho, Doni Bowo; Saksono, Budi; Gunawarman,

doi:10.1016/j.eurpolymj.2024.113171

Cited by 4 publications

(1 citation statement)

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“…Additionally, HA is employed in bioactive coatings [6,7], protein delivery media [8], drug carriers [9], and for the purification of nucleic acids during chromatographic analysis [10]. As the principal inorganic component of skeletal tissue, HA is often extracted from natural biological sources such as fish bone, goat bone, cattle bone, and chicken bone, with bovine bone being the most commonly used [11][12][13]. Bovine bone is widely used for HA production due to its abundance from the meat industry, similar mineral composition to human bone, and proven biocompatibility and efficacy in bone regeneration applications.…”

Section: Introductionmentioning

confidence: 99%

Enhancing Bioactivity and Mechanical Properties of Nano-Hydroxyapatite Derived from Oyster Shells through Hydrothermal Synthesis

Wu,

Hsu,

et al. 2024

Nanomaterials

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Nano-hydroxyapatite (nHA) demonstrates favorable biological activity, cell adhesion, cell proliferation, and osteoconductivity, making it highly valuable in biomedicine. It is extensively used as a bone substitute and in bone transplantation within the dental and orthopedic fields. This study employed oyster shells as a calcium source to synthesize nHA at 150 °C with various hydrothermal reaction durations (10 min, 1 h, 6 h, and 12 h). As a control, HA synthesized via a wet precipitation method for 1 h at room temperature was utilized. Subsequent material analyses, including XRD, FE-SEM, FTIR, and ICP-MS, were conducted, followed by comprehensive evaluations of the bioactivity, cell attachment, cell proliferation, and sintering properties of the synthesized nHA. The results indicated that nHA synthesized through the hydrothermal reaction produced nanoscale crystals, with the aspect ratio of nHA particles increasing with the duration of hydrothermal treatment. Notably, rod-like nHA particles became prominent with hydrothermal durations exceeding 6 h. nHA particles derived from oyster shells contained carbonate and trace elements (Na, Mg, K, and Sr), similar to constituents found in human hard tissue such as bone and teeth. The immersion of nHA synthesized at 150 °C for 1 h (HT2) in simulated body fluid (SBF) for 28 d led to the formation of a bone-like apatite layer on the surface, indicating the excellent bioactivity of the synthesized nHA. The cell culture results revealed superior cell attachment and proliferation for nHA (HT2). Following the sequential formation and sintering at 1200 °C for 4 h, HT2 ceramics exhibited enhanced microhardness (5.65 GPa) and fracture toughness (1.23 MPa·m0.5), surpassing those of human tooth enamel.

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Section: Introductionmentioning

confidence: 99%

Enhancing Bioactivity and Mechanical Properties of Nano-Hydroxyapatite Derived from Oyster Shells through Hydrothermal Synthesis

Wu,

Hsu,

et al. 2024

Nanomaterials

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Hydroxyapatite-based materials for adsorption, and adsorptive membrane process for heavy metal removal from wastewater: Recent progress, bottleneck and opportunities

Jaffar,

Othman,

Ismail

et al. 2024

Journal of the Taiwan Institute of Chemical Engineers

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Natural hydroxyapatite-based nanobiocomposites and their biomaterials-to-cell interaction for bone tissue engineering

Venkatesan,

Anchan,

Murugan

et al. 2024

Discover Nano

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Hydroxyapatite (HA) is an extensively used biomaterial for dental and orthopaedic applications because of its biocompatibility and biomimetic nature. HA is extensively used as a bone-graft substitute. HA bone graft substitutes of bovine or synthetic origins have been extensively studied. However, caprine-based HA has not been explored. In this study, we aimed to determine the utilization of goat bone-derived HA for commercial applications. HA from caprine bone and teeth was isolated using thermal calcination. The developed HA can be used as a bone graft substitute. Chemical characterization of the isolated HA was carried out using Fourier transform infrared spectroscopy, X-Ray Diffraction, Raman spectroscopy, scanning electron microscopy, and transmission electron microscopy. The biocompatibility and apatite formation of isolated HA were assessed using MG-63 cells, MC3T3-E1, L929 cells, MSCs, adipose derived stem cells, human dermal tissue derived fibroblast cells and osteoblast-like cell line, The studies demonstrate that HA support cell adhesion and osteogenic properties. To improve sheep, lamp, or caprine bone-derived HA, several other composites have been developed with MgO 2 , ZrO 2, ZnO 2, and other polymeric substances. 3D printed technology was used to develop a bioink using sheep-derived HA and printed the composite scaffold as a bone graft substitute. Furthermore, the biomedical applications of sheep-derived HA been studied in terms of their antimicrobial activity, bone-forming ability, and wound healing applications. Sheep-, goat-, and caprine-derived HA are still underutilized and require further research to develop commercial possibilities and sustainable raw materials for HA-based bone graft substitutes.

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Bovine hydroxyapatite for bone tissue engineering: Preparation, characterization, challenges, and future perspectives

Cited by 4 publications

References 100 publications

Enhancing Bioactivity and Mechanical Properties of Nano-Hydroxyapatite Derived from Oyster Shells through Hydrothermal Synthesis

Enhancing Bioactivity and Mechanical Properties of Nano-Hydroxyapatite Derived from Oyster Shells through Hydrothermal Synthesis

Hydroxyapatite-based materials for adsorption, and adsorptive membrane process for heavy metal removal from wastewater: Recent progress, bottleneck and opportunities

Natural hydroxyapatite-based nanobiocomposites and their biomaterials-to-cell interaction for bone tissue engineering

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