Bovine leukemia virus relation to human breast cancer: Meta-analysis

Gao, Andrew; Kouznetsova, Valentina L.; Tsigelny, Igor F.

doi:10.1016/j.micpath.2020.104417

Cited by 17 publications

(15 citation statements)

References 32 publications

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“…Інші види тварин можуть бути експериментально інфіковані BLV різними клінічними, гематологічними та імунологічними проявами (Sentsui et al, 1988;Krasnikova et al, 2019;Porta et al, 2019). Важливе значення має питання про спільність лейкозу великої рогатої худоби та людини (Gao et al, 2020). Механізми трансформації клітин ретровірусами людини і тварин, очевидно, принципово не відрізняються.…”

Section: вступunclassified

“…Лейкоз великої рогатої худоби не є специфічним лише для великої рогатої худоба, це інтерпретує передача вірусу вівцям та кролям з виявленням подібних клінічних, імунологічних та пато-морфологічних ознак, тому така схожість свідчить про можливу етіологічну спільність лейкозів ссавців, в тому числі й людини (Gao et al, 2020;Zyrianova & Kovalchuk, 2020). Висновки 1.…”

Section: обговоренняunclassified

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Pathogenetic aspects of rabbits’ experimental infection caused by bovine leukemia virus

Романишина

Feschenko

Rinyak³

et al. 2020

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Bovine leukemia virus (BLV) is an infectious disease of cattle, causing high economic losses worldwide, especially in the field of dairy farming. There is no common vision on the problem of interspecies transmission of BLV. Therefore, a detailed study of the etiologic relationship between leukemia in cattle and other animal species is relevant. Various laboratory animal models provide insight into the pathogenesis of viral infections. The article presents the research results of two series rabbits’ intravenous infection with bovine leukemia virus (BLV) using the culture antigen FLK-BLV and the blood of rabbits with clinical, hematological and immunological signs of viral tumor growth. Blood from all animals was taken from the ear vein after 14, 21, 30 days, and then monthly for six months: to study the morphological parameters of blood and to determine the titer of antibodies to BLV. Blood serum for the presence of antibodies to BLV was examined using a diagnostic kit for the indication of animals infected with the leukemia virus in an immunodiffusion reaction produced by LLC “SRE Veterinary Medicine”, Kharkiv. It was found that the stage of the BLV provirus in the blood leukogram of infected animals was characterized by pronounced lymphocytosis on the 21st day of the experiment. The highest concentration of antibodies to BLV in the blood serum was found on the 90th day after the administration of the virus-containing material, which disappeared from the blood on the 150–180th day after infection. In experimental rabbits, after five months for thirty days, in the absence of antibodies to leukemia in the blood serum, multiple tumors of a dense consistency began to develop throughout the body. Such clinical signs and changes in the of rabbits’ blood of the experimental group are characteristic of serologically positive cows on the hematological development stage of leukemic process and correlate with the results of domestic and foreign authors. The presence of a large number of lymphoblasts, as well as leukolysis cells, in the histological preparation of lymph nodes, lungs, heart and the accumulation of lymphocytes’ immature forms around the interlobular vessels of the liver, which were found in pathohistological studies of the experimental rabbits’ organs, may indicate the development of the leukemia process on early stage in them. The results obtained indicate the ability of BLV to overcome successfully the interspecies barrier upon parenteral ingestion of heterologous individuals from infected lymphocytes and in the form of a culture antigen.

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Section: обговоренняunclassified

Pathogenetic aspects of rabbits’ experimental infection caused by bovine leukemia virus

Романишина

Feschenko

Rinyak³

et al. 2020

SMLNUVMB

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“…In addition to affecting the longevity and overall health of the cows, BLV has also been found to affect milk production, which accounts to a loss of approximately 380 USD per cow per year [ 3 ]. Detection of BLV DNA in human blood or tumor samples has been linked to potential human health concerns, including breast cancer [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Tracing Viral Transmission and Evolution of Bovine Leukemia Virus through Long Read Oxford Nanopore Sequencing of the Proviral Genome

et al. 2021

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Bovine leukemia virus (BLV) causes Enzootic Bovine Leukosis (EBL), a persistent life-long disease resulting in immune dysfunction and shortened lifespan in infected cattle, severely impacting the profitability of the US dairy industry. Our group has found that 94% of dairy farms in the United States are infected with BLV with an average in-herd prevalence of 46%. This is partly due to the lack of clinical presentation during the early stages of primary infection and the elusive nature of BLV transmission. This study sought to validate a near-complete genomic sequencing approach for reliability and accuracy before determining its efficacy in characterizing the sequence identity of BLV proviral genomes collected from a pilot study made up of 14 animals from one commercial dairy herd. These BLV-infected animals were comprised of seven adult dam/daughter pairs that tested positive by ELISA and qPCR. The results demonstrate sequence identity or divergence of the BLV genome from the same samples tested in two independent laboratories, suggesting both vertical and horizontal transmission in this dairy herd. This study supports the use of Oxford Nanopore sequencing for the identification of viral SNPs that can be used for retrospective genetic contact tracing of BLV transmission.

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“…Discussion. In the last years topicality of the BLV is increasing in view of new information related to human breast cancer [21]. It leads to new research in this area and the development of new methods of BLV identification in cattle to eradicate infected animals [17,18,[22][23][24][25][26].…”

mentioning

confidence: 99%

Improving of the Nested PCR for Detection of Bovine Leukemia Virus

Іщенко¹,

Недосеков²,

Ishchenko³

et al. 2021

Mikrobiol. Z.

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Enzootic bovine leukosis caused by a bovine leukemia virus has a significant economic impact and is reported in World Organization for Animal Health(OIE). Aim. The purpose of our work was to improve the nested polymerase chain reaction (PCR) recommended by the OIE conducting it second-stage in real-time (RT) PCR. Such modification does not require the stage of gel electrophoresis and consequently reduces contamination risks and prevents false positive results. Methods. Primers that are recommended by the Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (OIE) were used for the first amplification stage. For the second stage of the proposed modification of nested PCR, the primers and probe were designed based on the alignment of the sequences envelope gene of different isolates of bovine leukemia virus including Ukrainian isolates. Amplification of the internal control was carried out for the second stage to prevent false negative results. Results. Comparative studies of 48 blood samples for bovine leukemia virus identification by a proposed nested RT-PCR, nested PCR recommended by the protocol of the OIE, and RT-PCR were conducted. The sample panel included both positive and negative samples. A 100% match of the results of the bovine leukemia virus presence in nested PCR proposed by the OIE and in our proposed nested RT-PCR was obtained. Comparative analysis of results that were obtained using the RT-PCR and the proposed nested RT-PCR showed that false-negative results in 5 samples and 3 doubtful results that require retesting were obtained by use of RT-PCR. The interpretation of the results using nested RT-PCR is more efficient than RT-PCR since the cycle threshold value of positive samples obtained using RT-PCR was in the range of 24–40 cycles, whereas in the case of nested RT-PCR using, the value of Ct was in the range of 4–20 cycles. Conclusions. Proposed nested PCR modification includes the combination of the OIE recommendation about nested PCR and the reduction of the risk of contamination by conducting the second stage in RT-PCR. Results of approbation of proposed nested RT-PCR give a reason to recommend it for the identification of bovine leukemia virus.

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Bovine leukemia virus relation to human breast cancer: Meta-analysis

Cited by 17 publications

References 32 publications

Pathogenetic aspects of rabbits’ experimental infection caused by bovine leukemia virus

Pathogenetic aspects of rabbits’ experimental infection caused by bovine leukemia virus

Tracing Viral Transmission and Evolution of Bovine Leukemia Virus through Long Read Oxford Nanopore Sequencing of the Proviral Genome

Improving of the Nested PCR for Detection of Bovine Leukemia Virus

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