Bovine milk RNases modulate pro-inflammatory responses induced by nucleic acids in cultured immune and epithelial cells

Gupta, Sandeep; Haigh, Brendan; Seyfert, Hans‐Martin; Griffin, Frank J; Wheeler, Thomas T.

doi:10.1016/j.dci.2016.11.015

Cited by 12 publications

(13 citation statements)

References 64 publications

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“…Bovine milk RNases can participate in the host response against infection both by direct antimicrobial action and immune response activation ( 182 ). A pro-inflammatory activity is observed in epithelial cells, which is mediated by nucleic acids ( 53 , 226 ). Both RNases can bind nucleic acids, and milk RNase5 induces cytokine release in leukocytes ( 53 ).…”

Section: The Rnase a Superfamilymentioning

confidence: 99%

“…Both RNases can bind nucleic acids, and milk RNase5 induces cytokine release in leukocytes ( 53 ). Recognition of foreign pathogen nucleic acids may facilitate the activation of pattern recognition receptors and promote a pro-inflammatory response ( 226 ). Interestingly, the RNase immuno-stimulatory activity is also dependent on the protein catalytic activity ( 226 ).…”

Section: The Rnase a Superfamilymentioning

confidence: 99%

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Immune Modulation by Human Secreted RNases at the Extracellular Space

et al. 2018

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The ribonuclease A superfamily is a vertebrate-specific family of proteins that encompasses eight functional members in humans. The proteins are secreted by diverse innate immune cells, from blood cells to epithelial cells and their levels in our body fluids correlate with infection and inflammation processes. Recent studies ascribe a prominent role to secretory RNases in the extracellular space. Extracellular RNases endowed with immuno-modulatory and antimicrobial properties can participate in a wide variety of host defense tasks, from performing cellular housekeeping to maintaining body fluid sterility. Their expression and secretion are induced in response to a variety of injury stimuli. The secreted proteins can target damaged cells and facilitate their removal from the focus of infection or inflammation. Following tissue damage, RNases can participate in clearing RNA from cellular debris or work as signaling molecules to regulate the host response and contribute to tissue remodeling and repair. We provide here an overall perspective on the current knowledge of human RNases’ biological properties and their role in health and disease. The review also includes a brief description of other vertebrate family members and unrelated extracellular RNases that share common mechanisms of action. A better knowledge of RNase mechanism of actions and an understanding of their physiological roles should facilitate the development of novel therapeutics.

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Section: The Rnase a Superfamilymentioning

confidence: 99%

Section: The Rnase a Superfamilymentioning

confidence: 99%

Immune Modulation by Human Secreted RNases at the Extracellular Space

et al. 2018

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“…The conditions found in biological fluids are highly unfavorable for nucleic acids. These include a high ribonuclease activity (Dalaly, Eitenmiller, Friend, & Shahani, ; Gupta, Haigh, Seyfert, Griffin, & Wheeler, ; Ramaswamy, Swamy, & Das, ) that suggested, at first, that microRNAs are intracellular compounds and only impact gene expression of the cells that produce them (Leung, ).…”

Section: Circulating Micrornas: the Paradigm Shiftmentioning

confidence: 99%

“…Milk microRNAs are found in all milk fractions and other dairy preparations and are ingested by infants or adult consumers on a daily basis (Melnik, ; Oh et al., ). Without protection, milk microRNAs would be degraded during digestion or by the high levels of RNAse found in milk (Gupta et al., ; Ramaswamy et al., ) resulting in a very poor bioaccessibility.…”

Section: Milk Micrornas In Health and Diseasementioning

confidence: 99%

Milk MicroRNAs in Health and Disease

Benmoussa

Provost

2019

Comp Rev Food Sci Food Safe

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MicroRNAs are small noncoding RNAs responsible for regulating 40% to 60% of gene expression at the posttranscriptional level. The discovery of circulating microRNAs in several biological fluids opened the path for their study as biomarkers and long‐range cell‐to‐cell communication mediators. Their transfer between individuals in the case of blood transfusion, for example, and their high enrichment in milk have sparked the interest for microRNA transfer through diet, especially from mothers to infants during breastfeeding. The extension of such paradigm led to the study of milk microRNAs in the case of cow or goat milk consumption in adults. Here we provide a comprehensive critical review of the key findings surrounding milk microRNAs in human, cow, and goat milk among other species. We discuss the data on their biological properties, their use as disease biomarkers, their transfer between individuals or species, and their putative or verified functions in health and disease of infants and adult consumers. This work is based on all the literature available and integrates all the results, theories, debates, and validation studies available so far on milk microRNAs and related areas of investigations. We critically discuss the limitations and outline future aspects and avenues to explore in this rapidly growing field of research that could impact public health through infant milk formulations or new therapies. We hope that this comprehensive review of the literature will provide insight for all teams investigating milk RNAs’ biological activities and help ensure the quality of future reports.

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“…One milliliter of the heated sample was added to cooked meat-glucose-starch medium (11) and incubated anaerobically at 35°C for 48 h. The growth suspension was serially diluted, plated on Shahidi-Ferguson agar (12), and incubated anaerobically for 24 h to yield pure colonies. Genomic DNA was extracted from the pure cultures grown in tryptic soy broth (Fort Richard Laboratories, New Zealand) by using a phenol-chloroform extraction method (13). The quality and concentration of DNA were determined using a Qubit 2.0 fluorometer (Thermo Fisher Scientific, USA).…”

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confidence: 99%

Draft Genome Sequence of Clostridium senegalense Strain AGRFS4, Isolated from a Dairy Farm in New Zealand

Gupta

Jáuregui

Nawarathana

et al. 2020

Microbiol Resour Announc

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We report the draft genome sequence of a new Clostridium senegalense strain, AGRFS4, which was isolated from a dairy farm environment in the Manawatu region in New Zealand. The genome is 3.98 Mb, with a GC content of 27%. The genome sequence was found to be 86.6% similar to that of the type strain Clostridium senegalense JC122. Until now, no Clostridium senegalense strain from New Zealand has been reported.

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Bovine milk RNases modulate pro-inflammatory responses induced by nucleic acids in cultured immune and epithelial cells

Cited by 12 publications

References 64 publications

Immune Modulation by Human Secreted RNases at the Extracellular Space

Immune Modulation by Human Secreted RNases at the Extracellular Space

Milk MicroRNAs in Health and Disease

Draft Genome Sequence of Clostridium senegalense Strain AGRFS4, Isolated from a Dairy Farm in New Zealand

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