Bovine oocyte vitrification before or after meiotic arrest: effects on ultrastructure and developmental ability

Díez, C.; Duque, Paloma; Gómez, E.; Hidalgo, C.O.; Tamargo, Carolina; Rodríguez, Aida; Fernández, Lina; Varga, Santiago de la; Fernández, Alicia Calleja; Facal, N.; Carbajo, M.

doi:10.1016/j.theriogenology.2004.11.023

Cited by 72 publications

(55 citation statements)

References 55 publications

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“…The achievements of vitrification with our newly developed carrier are comparable to results achieved by following research teams using similar vitrification protocol and open carriers (Luna et al 2001;Diez et al 2002;Vieira et al 2002;Modina et al 2004;Albarracín et al 2005;Morató et al 2008aMorató et al , 2008bMorató et al , 2008cand Hou et al 2009). Hence, we can declare this new carrier a safe variant for bovine oocyte vitrification, which is inexpensive to manufacture from affordable components.…”

Section: Discussionsupporting

confidence: 78%

Closed system for bovine oocyte vitrification

Ševelová

Lopatářová

2012

Acta Vet. Brno

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The aim of our study was to develop a vitrification carrier for bovine oocyte cryopreservation. The carrier was to be cheap enough, elementary in its construction and meet contemporary requirements for a safe closed system. In a closed system, a cell is prevented from direct exposure to liquid nitrogen, thus minimizing the risk of cross-contamination. Furthermore, two questions regarding the proper vitrification technique were resolved: if it is necessary to partially denude the oocytes before the vitrification process or whether intact cumulus oocyte complexes should be frozen; and if it is more advantageous to preheat the vitrification solutions to female body temperature (39 °C) or to keep them at room temperature. Our results show that it is better to partially denude the oocytes prior to vitrification because cryopreserved intact cumulus oocyte complexes often proved dark, non-homogeneous or fragmented cytoplasm after warming, with many of them having visibly widened perivitelline spaces or fractured zonae pellucidae as a result of extensive damage during vitrification. Consequently, intact cumulus oocyte complexes showed significantly lower numbers of cleavage stage embryos on Day 3 compared to partially denuded oocytes (7.4% and 26%, respectively). On the other hand, the survival rate and following development of fertilized oocytes in preheated vitrification solution were equal to results reached at room temperature conditions. In conclusion, results achieved with the newly developed carrier were comparable to previously published studies and therefore they could be recommended for common use.

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Section: Discussionsupporting

confidence: 78%

Closed system for bovine oocyte vitrification

Ševelová

Lopatářová

2012

Acta Vet. Brno

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“…Changes in the structure of the cytoskeleton, mitochondria, cortical granules and nucleoli have been observed in OPS vitrified-warmed bovine oocytes (Hyttel et al, 2000). Cytoplasmic organelle degeneration, lack of communication between the cumulus cells and the oocyte or alteration in the migration patterns of cortical granules may be factors involved in oocyte degeneration after cryopreservation (Diez et al, 2005). It is likely that the reasons for reduced development observed in our experiments are similar to those found in the bovine.…”

Section: Discussionsupporting

confidence: 72%

“…OPS vitrified bovine oocytes have significantly lower developmental ability than non-treated oocytes (Diez et al, 2005). Changes in the structure of the cytoskeleton, mitochondria, cortical granules and nucleoli have been observed in OPS vitrified-warmed bovine oocytes (Hyttel et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Piezo-actuated zona-drilling improves the fertilisation of ops vitrified mouse oocytes

Meng

Tong-yi

et al. 2007

Acta Veterinaria Hungarica

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The present study was designed to investigate fertilisation of open pulled straw (OPS) vitrified mouse oocytes drilled with piezo-micromanipulation method and their subsequent in vitro and in vivo developmental capacity. Ovulated mouse oocytes were vitrified using the OPS method. After warming, the zona pellucida of a group of vitrified-warmed oocytes was drilled by piezo-micromanipulation. Groups of (a) vitrified, (b) vitrified/drilled and (c) fresh control oocytes were fertilised in vitro. The fertilisation rate of vitrified-warmed oocytes was significantly lower than that of fresh oocytes (45.0 ± 12.6% vs. 85.2 ± 6.8%, P < 0.05), and was significantly improved by zona-drilling (85.4 ± 7.3%). However, blastocyst formation rates of the vitrified and vitrified/drilled groups were significantly lower than those of the fresh controls (65.7 ± 7.0% and 66.4 ± 2.5% vs. 86.6 ± 4.3%, respectively, P < 0.05). The cell number of blastocysts from the vitrified/drilled or the vitrified group was not different from that of the controls. Embryo transfer resulted in pregnancy in all three groups, but the rate of development to term was lower in the vitrified/drilled or vitrified groups than in the controls (16.6 ± 0.7% or 36.0 ± 2.4% vs. 51.3 ± 2.9%, respectively). Our results demonstrated that zona-drilling with piezo-micromanipulation could improve fertilisation in OPS vitrified mouse oocytes but did not increase the overall number of vitrified oocytes developing to term.

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“…A significant improvement in bovine oocyte survival was obtained when EM grids were used to provide physical support [12]. Thereafter, OPS [6,26,27] and nylon mesh [14,28] were introduced as containers for freezing of immature bovine oocytes. In the present study, bovine immature oocytes were vitrified using the microdrop vitrification method.…”

Section: Discussionmentioning

confidence: 99%

Vitrification of Immature Bovine Oocytes by the Microdrop Method

et al. 2007

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Abstract. This study was conducted to determine the optimal vitrification conditions for immature bovine oocytes using the microdrop method. In experiment 1, the optimal pre-equilibration period for microdrop vitrification was examined. The maturation rate of vitrified oocytes with a 3 min first preequilibration period (41.1%) was higher than that of vitrified oocytes with a 0 min first preequilibration period (21.4%), and the values of those with a 1 (33.9%) or 5 min (27.4%) first preequilibration period were intermediate. The value for a 1 min second pre-equilibration period (44.4%) was significantly higher (P<0.05) than those for a 0.5 (28.6%) and 2 min (21.4%) second preequilibration period. In experiment 2, the distribution of microtubules in matured oocytes was investigated. There was no difference among the first pre-equilibration times in terms of the rates of normal spindles in vitrified oocytes. However, this value was significantly higher (P<0.05) in the 1 min group (52.8%) compared with the 0.5 (16.7%) and 2 min groups (12.3%). In experiment 3, we investigated the developmental capacity of immature bovine oocytes vitrified under optimal preequilibration conditions (3 min and 1 min for the first and second pre-equilibrations, respectively). Although the total fertilization rates were significantly lower (P<0.05) in the vitrified oocytes (65.6%) compared with the control oocytes (92.4%), there was no difference in the rate of normal fertilization (2PN) between the vitrified (78.6%) and control (82.0%) oocytes. Cleavage and blastocyst rates were significantly lower (P<0.05) in vitrified oocytes (55.7 and 2.3%) than in control oocytes (84.4 and 34.7%). Thus, these results indicated that immature bovine oocytes can survive after microdrop vitrification and subsequently can be cultured to mature oocytes capable of undergoing fertilization in vitro and developing into blastocysts.

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Bovine oocyte vitrification before or after meiotic arrest: effects on ultrastructure and developmental ability

Cited by 72 publications

References 55 publications

Closed system for bovine oocyte vitrification

Closed system for bovine oocyte vitrification

Piezo-actuated zona-drilling improves the fertilisation of ops vitrified mouse oocytes

Vitrification of Immature Bovine Oocytes by the Microdrop Method

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