Bovine polyomavirus, a cell-transforming virus with tumorigenic potential

Schuurman, Rob; Strien, A. van; Steenis, Bert van; Noordaa, J. van der; Sol, C. J. A.

doi:10.1099/0022-1317-73-11-2871

Cited by 7 publications

(5 citation statements)

References 25 publications

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“…The 619 amino acidencoding ORF of the splice I/II mRNA was expected to encode a protein of approximately 70K. This has recently been proven by Western blotting (Schuurman et al, 1992).…”

Section: Discussionmentioning

confidence: 95%

“…Such an interaction might be an important step in cell transformation (Chen & Paucha, 1990). In this respect, it is worth mentioning that primary rodent cells can be transformed by the early gene products of BPyV (Schuurman et al, 1992).…”

Section: Discussionmentioning

confidence: 99%

“…Cell cultures of embryonic mouse cells (ME cells) and embryonic mouse cells transformed by the early region of bovine polyomavirus (ME-RBPy clones l, 2 and 3; Schuurman et al, 1992) were cultured in Iscove's modified Dulbecco's medium supplemented with 8% FCS. The FCS used for cell culture had previously been screened for the presence of BPyV using a BPyV-specific PCR assay (Schuurman et al, 1991).…”

Section: And Othersmentioning

confidence: 99%

“…Six days after infection, when cytopathology was observed throughout the cell culture, total cellular RNA was isolated from monkey kidney cells infected with BPyV (0-1 TCIDso/cell). Total cellular RNA was also isolated from subconfluent cultures of either BPyV-transformed murine embryo cells (Schuurman et al, 1992) or from untransformed (ME) cells.…”

Section: And Othersmentioning

confidence: 99%

See 3 more Smart Citations

Analysis of splice sites in the early region of bovine polyomavirus: evidence for a unique pattern of large T mRNA splicing

Schuurman

Jacobs

Strien

et al. 1992

Journal of General Virology

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“…The 619 amino acidencoding ORF of the splice I/II mRNA was expected to encode a protein of approximately 70K. This has recently been proven by Western blotting (Schuurman et al, 1992).…”

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: And Othersmentioning

confidence: 99%

Section: And Othersmentioning

confidence: 99%

See 2 more Smart Citations

Analysis of splice sites in the early region of bovine polyomavirus: evidence for a unique pattern of large T mRNA splicing

Schuurman

Jacobs

Strien

et al. 1992

Journal of General Virology

Self Cite

View full text Add to dashboard Cite

“…It can transform (cause foci of overgrowth of) tissue cultures of rodent kidney cells. These transformed cells, inoculated into immunocompromised rats, lead within three weeks to the appearance of macroscopically visible tumours at the site of inoculation (Schuurman et al, 1992).…”

Section: Cancer Virusesmentioning

confidence: 99%

Reflections on viruses and cancer

Darcel¹

1994

Vet Res Commun

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In recent years human and animal cancers have increasingly been shown to have a viral component in their aetiology. Oncogenic viruses will continue to be discovered although with certain cancers there is also an important environmental component, and with others--congenital cancers and cancers of early childhood--an important genetic component. There is thus the probability that 'cancer' may not be an entity. Rather it may be a syndrome, the phenotypic expression of alteration of cellular metabolism, differentiation and cell death. More information is needed on the mathematics of cell division and destruction, in vivo and in vitro, and the involvement of 'biological clocks', i.e. ageing processes. These data, when available, should help us to understand better the nature of cancer and lead us to more effective methods of prevention and cure.

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Cell Culture Contamination

Stacey

2011

Methods in Molecular Biology

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Microbial contamination is a major issue in cell culture, but there are a range of procedures which can be adopted to prevent or eliminate contamination. Contamination may arise from the operator and the laboratory environment, from other cells used in the laboratory, and from reagents. Some infections may present a risk to laboratory workers: containment and aseptic technique are the key defence against such risks. Remedial management of suspected infection may simply mean discarding a single potentially infected culture. However, if a more widespread problem is identified, then all contaminated cultures and associated unused media that have been opened during this period should be discarded, equipment should be inspected and cleaned, cell culture operations reviewed, and isolation from other laboratories instituted until the problem is solved. Attention to training of staff, laboratory layout, appropriate use of quarantine for new cultures or cell lines, cleaning and maintenance, and quality control are important factors in preventing contamination in cell culture laboratories.

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Bovine polyomavirus, a cell-transforming virus with tumorigenic potential

Cited by 7 publications

References 25 publications

Analysis of splice sites in the early region of bovine polyomavirus: evidence for a unique pattern of large T mRNA splicing

Analysis of splice sites in the early region of bovine polyomavirus: evidence for a unique pattern of large T mRNA splicing

Reflections on viruses and cancer

Cell Culture Contamination

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