Bovine respiratory disease.

Nicholas, Robin A.J.; Ayling, Roger D.

doi:10.1079/9780851990125.0132

Cited by 32 publications

(45 citation statements)

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“…M bovis respiratory disease in cattle was most commonly diagnosed in young animals (Fig C), consistent with previous knowledge 2. Since 2014 the rise in the number of diagnoses has been predominantly in the pre- and postwean age groups, with the disease most common in the postweaning period.…”

Section: Surveillance Trends In Gbsupporting

confidence: 89%

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Mycoplasma bovis investigations in cattle

2018

Veterinary Record

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Section: Surveillance Trends In Gbsupporting

confidence: 89%

“…M bovis pneumonia has previously been reported to be higher during colder seasons,2 with fluctuating temperatures and quality of housing likely contributory factors 2,4. Closer contact when housed also increases likelihood of transmission to other cattle in the herd.…”

Section: Surveillance Trends In Gbmentioning

confidence: 99%

Mycoplasma bovis investigations in cattle

2018

Veterinary Record

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“…The control of M. bovis infections relies strongly on antimicrobial therapy, which has variable success rates in the field (3). Vaccination has been used in the early stages of cattle development and is mostly based on autogenous vaccines, which limits their use and the potential for widespread control of M. bovis infections (3).…”

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confidence: 99%

Global Multilocus Sequence Typing Analysis of Mycoplasma bovis Isolates Reveals Two Main Population Clusters

et al. 2015

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Mycoplasma bovis is a major bovine pathogen associated with bovine respiratory disease complex and is responsible for substantial economic losses worldwide. M. bovis is also associated with other clinical presentations in cattle, including mastitis, otitis, arthritis, and reproductive disorders. To gain a better understanding of the genetic diversity of this pathogen, a multilocus sequence typing (MLST) scheme was developed and applied to the characterization of 137 M. bovis isolates from diverse geographical origins, obtained from healthy or clinically infected cattle. After in silico analysis, a final set of 7 housekeeping genes was selected (dnaA, metS, recA, tufA, atpA, rpoD, and tkt). MLST analysis demonstrated the presence of 35 different sequence types (STs) distributed in two main clonal complexes (CCs), defined at the double-locus variant level, namely, CC1, which included most of the British and German isolates, and CC2, which was a more heterogeneous and geographically distant group of isolates, including European, Asian, and Australian samples. Index of association analysis confirmed the clonal nature of the investigated M. bovis population, based on MLST data. This scheme has demonstrated high discriminatory power, with the analysis showing the presence of genetically distant and divergent clusters of isolates predominantly associated with geographical origins.M ycoplasma diseases cause substantial economic losses, particularly in intensively farmed cattle production systems worldwide, as a result of poor growth, morbidity, and deaths, as well as the costs associated with increased control and prophylactic measures. Mycoplasma bovis has increasingly been recognized as one of the main pathogens involved in the bovine respiratory disease complex, on its own or in association with other respiratory pathogens (1). M. bovis can also be found in association with mastitis, in which outbreaks can affect more than 20% of the cows in a herd, regardless of the stage of lactation, and infections are usually refractory to treatment. Arthritis and otitis have also been associated with M. bovis, usually appearing once pneumonia or mastitis is already established in the herd (1, 2). The control of M. bovis infections relies strongly on antimicrobial therapy, which has variable success rates in the field (3). Vaccination has been used in the early stages of cattle development and is mostly based on autogenous vaccines, which limits their use and the potential for widespread control of M. bovis infections (3).Taking into consideration the limited tools available for M. bovis disease management, the development of a dependable molecular typing scheme able to offer robust and reproducible epidemiological information would provide a valuable addition to control measures targeting this pathogen. M. bovis isolates have been characterized previously using multiple molecular typing methods, including amplified fragment length polymorphism (AFLP) analysis (4), random amplified polymorphic DNA (RAPD) analysis (5), pulsed-fiel...

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“…These three Mycoplasma’s are associated with BRD [20] and application of the multiplex bacterial real-time PCR and the triplex Mycoplasma real-time PCR together improves detection of BRD-associated pathogens further.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a multiplex real-time PCR for detection of four bacterial agents commonly associated with bovine respiratory disease in bronchoalveolar lavage fluid

et al. 2017

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Background Pasteurella multocida, Mannheimia haemolytica, Histophilus somni and Trueperella pyogenes are four bacterial agents commonly associated with bovine respiratory disease (BRD). In this study a bacterial multiplex real-time PCR (the RespoCheck PCR) was evaluated for the detection in bronchoalveolar lavage fluid (BALF) of these four bacterial agents.ResultsThe analytical sensitivity of the multiplex real-time PCR assay determined on purified DNA and on bacterial cells of the four target pathogens was one to ten fg DNA/assay and 4 × 10−1 to 2 × 100 CFU/assay. The analytical specificity of the test was, as evaluated on a collection of 118 bacterial isolates, 98.3% for M. haemolytica and 100% for the other three target bacteria. A set of 160 BALF samples of calves originating from ten different herds with health problems related to BRD was examined with bacteriological methods and with the RespoCheck PCR. Using bacteriological examination as the gold standard, the diagnostic sensitivities and specificities of the four bacterial agents were respectively between 0.72 and 1.00 and between 0.70 and 0.99. Kappa values for agreement between results of bacteriological examination and PCRs were low for H. somni (0.17), moderate for P. multocida (0.52) and M. haemolytica (0.57), and good for T. pyogenes (0.79). The low and moderate kappa values seemed to be related to limitations of the bacteriological examination, this was especially the case for H. somni.ConclusionIt was concluded that the RespoCheck PCR assay is a valuable diagnostic tool for the simultaneous detection of the four bacterial agents in BALF of calves.

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Bovine respiratory disease.

Cited by 32 publications

References 0 publications

Mycoplasma bovis investigations in cattle

Mycoplasma bovis investigations in cattle

Global Multilocus Sequence Typing Analysis of Mycoplasma bovis Isolates Reveals Two Main Population Clusters

Evaluation of a multiplex real-time PCR for detection of four bacterial agents commonly associated with bovine respiratory disease in bronchoalveolar lavage fluid

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