Bovine Semen Quality Control in Artificial Insemination Centers

Vincent, Patrick W.; Underwood, S.L.; Dolbec, Catherine; Bouchard, Nadine; Kroetsch, Tom; Blondin, Patrick

doi:10.1002/9781118833971.ch74

Cited by 39 publications

(54 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The use of CASA that allows a detailed quantitative measurement of individual sperm cell motility should provide more reliable results than mass sperm motility to predict fertilizing capacity of semen doses (Vincent et al, 2014). However, studies linking CASA parameters to fertility have not clearly demonstrated a greater predictive fertility capability for bulls (Kjaestad et al, 1993;Farrell et al, 1998;Januskauskas et al, 1999;Gillan et al, 2008).…”

Section: Resultsmentioning

confidence: 97%

Mass sperm motility is associated with fertility in sheep

David

Kohnke

Lagriffoul

et al. 2015

Animal Reproduction Science

View full text Add to dashboard Cite

The study was to focus on the relationship between wave motion (mass sperm motility, measured by a mass sperm motility score, manually assessed by artificial insemination (AI) center operators) and fertility in male sheep. A dataset of 711,562 artificial inseminations performed in seven breeds by five French AI centers during the 2001-2005 time period was used for the analysis. Factors influencing the outcome of the insemination, which is a binary response observed at lambing of either success (1) or failure (0), were studied using a joint model within each breed and AI center (eight separate analyses). The joint model is a multivariate model where all information related to the female, the male and the insemination process were included to improve the estimation of the factor effects. Results were consistent for all analyses. The male factors affecting AI results were the age of the ram and the mass motility. After correction for the other factors of variation, the lambing rate increased quasi linearly from three to more than ten points with the mass sperm motility score depending on the breed and the AI center. The consistency of the relationship for all breeds indicated that mass sperm motility is predictive of the fertility resulting when sperm are used from a specific ejaculate. Nonetheless, predictability could be improved if an objective measurement of mass sperm motility were available as a substitute for the subjective scoring currently in use in AI centers.

show abstract

Section: Resultsmentioning

confidence: 97%

Mass sperm motility is associated with fertility in sheep

David

Kohnke

Lagriffoul

et al. 2015

Animal Reproduction Science

View full text Add to dashboard Cite

show abstract

“…Currently, it is the most objective and detailed method, with improved repeatability and sensitivity in determining the motility not only of the semen sample, but also of individual sperms, and can even characterize them to sperm subpopulations within a sample [1]. These sperm subpopulations are based on the kinematics data, which define the movement of the sperm based on its distance travelled, velocity, head…”

mentioning

confidence: 99%

Sperm subpopulations, buffalo semen, sperm kinematics, cryopreservation, computer assisted sperm analyzer.

Maylem¹,

Leoveras²,

Venturina³

et al. 2017

JAST-A

View full text Add to dashboard Cite

Abstract:This study aimed to identify and characterize the sperm subpopulations existing in water buffalo semen using a computer assisted sperm analyzer (CASA), as well as assess the effects of cryopreservation on the sperm subpopulation structure and evaluate bull variability. The semen of eight Bulgarian Murrah bulls was collected by four times in an interval of one week each. The semen was cryopreserved following a standard protocol and sperm kinematics was assessed. Clustering methods were applied to individual sperms, forming two significantly different (P < 0.05) subpopulations (P 1 and P 2 ). Subpopulation P 1 represents those spermatozoa that moved most rapidly and progressively (46.29%), and subpopulation P 2 includes spermatozoa with relatively low velocity or poorly motile but with high progressiveness (53.41%). There was a decline on the population of P 1 sperms from fresh (52.52%), pre-freeze (45.73%) to post-thaw (35.17%) stages and significant difference on the sperm kinematics between P 1 and P 2 . A significant decline in the values of distance, velocity and amplitude of lateral head (ALH) parameters were observed at post-thaw stage, while an increase was observed on trajectory and beat cross frequency (BCF) kinematics. Values of sperm kinematics were also significantly different (P < 0.05) among all bulls. The frequency distribution of spermatozoa on both subpopulations P 1 and P 2 was quite similar for all bulls in pre-freeze and post-thaw stages, but with significant (P < 0.05) variability on fresh stage. Bulls with the highest maintained frequency of P 1 sperms are denoted as good freezer bulls. In sum, kinematic characterization of water buffalo sperm and clustering into subpopulation enable to identify bulls that are more resistant to cryopreservation and production of quality semen for genetic propagation.

show abstract

“…A fertilidade varia substancialmente entre os touros. Em geral, os métodos para estimar a fertilidade são melhores para a identificação de touros com baixa fertilidade do que para classificar touros com boa ou excelente fertilidade (KASTELIC, 2013 (BERLINGUER et al, 2009;VINCENT et al, 2012;LI et al, 2014;OLIVEIRA et al, 2014 …”

Section: Fertilidade Do Tourounclassified

“…Em níveis fisiológicos, o NO participa da capacitação espermática, reação acrossomal e efeito antiapoptótico (WANG et al, 2014, ROESSNER et al, 2010. Coincidindo também com Franco (2013) A relação entre qualidade do sêmen de touros com a fertilidade a campo, seja por resultado da taxa de prenhez, ou pela taxa de não retorno ao estro (NRR), tem sido foco de muitas pesquisas (CHRISTENSEN et al, 2005;VINCENT et al, 2012;OLIVEIRA et al, 2014b;SELLEM et al, 2015, SANTOS, 2016a. Entretanto, os resultados são controversos.…”

Section: Estatística Descritivaunclassified

“…A fertilidade do macho está intrinsecamente ligada à qualidade do sêmen, porém, o método de predição da fertilidade de uma amostra seminal in vitro não é simples, sendo que diferentes características do sêmen podem limitar o potencial fecundante (AMANN, 1989). Fatores dependentes do indivíduo, do próprio espermatozoide, de como o sêmen foi processado e de como a amostra foi avaliada podem influenciar na predição da fertilidade de uma amostra de sêmen (AMANN; HAMMERSTEDT, 1993;VINCENT et al, 2012). Estimativas ou predições do potencial de fertilidade do sêmen tem sido difíceis, principalmente pela heterogeneidade do ejaculado, devido à presença de distintas subpopulações com diferentes características funcionais dentro do ejaculado, que podem contribuir no processo de fecundação (RODRÍGUEZ-MARTÍNEZ, 2006).…”

Section: Introductionunclassified

See 1 more Smart Citation

<b>Impacto da qualidade espermática sobre a fertilidade <i>in vivo</i> em bovinos:</b> contribuição de marcadores mitocondriais e subpopulações espermáticas

Florez-Rodriguez¹

View full text Add to dashboard Cite

Esta tese é dedicada aos meus anjos da guarda na terra:Minha mãe, meu amor, pelo amor incondicional, pelas sábias palavras em todos os momentos da minha vida e pelo seu fervor nas orações para que o senhor Deus me encha de bênçãos.Minha irmã Sandra, minha adoração, meu melhor presente da vida, o amor mais leal.Meu pai, sempre presente, pelo apoio e suporte incondicional, pela dedicação e o esforço a suas filhas.Minha família, minha fonte de fortaleza e palavras de alento. À minha família, meus pais Myriam e Alejandro, e minha irmã Sandra.À minha orientadora Profa. Dra. Eneiva Carla Carvalho Celeghini pela paciência, dedicação, pelo apoio em todo momento e pelo interesse por meu crescimento profissional.Mas, principalmente porque ao longo desses anos você não só me ensinou à ser uma pesquisadora, mas também um ser humano melhor, além que, você é meu melhor exemplo de trabalho, com coragem e determinação. Muito carinho e muita gratidão. The objective of this study was to evaluate the sperm characteristics of bovine semen and the impact on fertility when used in an IATF program. Four experiments were carried out and described in the form of scientific articles. In article 1. We compared the semen quality determined by subjective and objective methods for the in vitro sperm analysis of bovine semen. According to the results, three groups were analyzed: high quality (A), good quality (B) and high quality (A), Questionable quality (Q). Subsequently, the results of objective analyzes using fluorescence probes by epifluorescence microscopy were analyzed to determine the integrity of plasma, acrosomal membrane and mitochondrial membrane potential (% PIAIA); And using the computerized sperm motility evaluation system (CASA), the effect was evaluated using ANOVA and Tukey 5%. Afterwards, 67 matches were used to determine the agreement to determine seminal quality according to three methods of seminal evaluation: (1) Subjective analysis considering subjective motility, vigor and sperm morphology (2) Analysis by CASA taking into consideration the TM, VCL, VSL and VAP and (3) Analysis by fluorescent probes considering % PIAIA. Pearson correlation analysis and concordance analysis (Kappa significance) were performed. In article 2, 18 sets of semen were classified according to the fertility index of each bull in two groups: High fertility (n = 9) and Low fertility (n = 9). The classification of the score was provided by the central office based on data from 33,198 services by IATF. The semen were subjected to conventional analyzes, CASA, fluorescent probe analysis to evaluate the percentage of PIAIA in epifluorescence microscopy and production of mitochondrial markers by fluorescent probes in flow cytometry. Pearson correlation analyzes were performed on all sperm characteristics and effects between fertility groups were evaluated using ANOVA and Tukey 5%. In article 3 the objective was to determine the relationship between sperm quality measured by the production of mitochondrial markers and the integrity of the spermatic stru...

show abstract

Bovine Semen Quality Control in Artificial Insemination Centers

Cited by 39 publications

References 61 publications

Mass sperm motility is associated with fertility in sheep

Mass sperm motility is associated with fertility in sheep

Sperm subpopulations, buffalo semen, sperm kinematics, cryopreservation, computer assisted sperm analyzer.

<b>Impacto da qualidade espermática sobre a fertilidade <i>in vivo</i> em bovinos:</b> contribuição de marcadores mitocondriais e subpopulações espermáticas

Contact Info

Product

Resources

About