Bowline, a novel protein localized to the presomitic mesoderm, interacts with Groucho/TLE in Xenopus

Kondow, Akiko; Hitachi, Keisuke; Ikegame, Tempei; Asashima, Makoto

doi:10.1387/ijdb.052138ak

Cited by 25 publications

(33 citation statements)

References 43 publications

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“…Through this screening process, several somitogenesis-related genes, such as paraxial protocadherin and mesogenin 1 (msgn1), as well as a novel gene bowline (Kim et al, 2000;Yoon et al, 2000;Kondow et al, 2006), were isolated. Two bowline-related genes, ledgerline and xRipply3, were also isolated from X. laevis (Chan et al, 2006;Hitachi et al, 2008).…”

Section: Identification and Characterization Of Novel Somitogenesis-rmentioning

confidence: 99%

“…For formation of the somite boundaries, bowline, ledgerline, and their counterpart in zebrafish, ripply1, are required (Kawamura et al, 2005;Chan et al, 2006;Kondow et al, 2006). Knockout of the murine Ripply2 gene results in segmental defects and rostralization of the somites (Chan et al, 2007;Morimoto et al, 2007).…”

Section: Identification and Characterization Of Novel Somitogenesis-rmentioning

confidence: 99%

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In vitro organogenesis from undifferentiated cells in Xenopus

Asashima

Ito

Chan

et al. 2009

Developmental Dynamics

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Amphibians have been used for over a century as experimental animals. In the field of developmental biology in particular, much knowledge has been accumulated from studies on amphibians, mainly because they are easy to observe and handle. Xenopus laevis is one of the most intensely investigated amphibians in developmental biology at the molecular level. Thus, Xenopus is highly suitable for studies on the mechanisms of organ differentiation from not only a single fertilized egg, as in normal development, but also from undifferentiated cells, as in the case of in vitro organogenesis. Based on the established in vitro organogenesis methods, we have identified many genes that are indispensable for normal development in various organs. These experimental systems are useful for investigations of embryonic development and for advancing regenerative medicine. Developmental Dynamics 238:1309 -1320, 2009.

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Section: Identification and Characterization Of Novel Somitogenesis-rmentioning

confidence: 99%

Section: Identification and Characterization Of Novel Somitogenesis-rmentioning

confidence: 99%

In vitro organogenesis from undifferentiated cells in Xenopus

Asashima

Ito

Chan

et al. 2009

Developmental Dynamics

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“…We and others previously identified a novel protein family, Bowline/Ripply, from the mouse, African clawed frog (Xenopus laevis), zebrafish, and amphioxus (Chan et al, 2007;Chan et al, 2006;Kawamura et al, 2005;Kondow et al, 2006;Li et al, 2006;Morimoto et al, 2007). In vertebrates, two bowline/Ripply family genes have been identified and characterized to date.…”

Section: Introductionmentioning

confidence: 99%

“…In vertebrates, two bowline/Ripply family genes have been identified and characterized to date. The expression region of bowline, which is a member of the bowline/ Ripply family gene in Xenopus, is restricted to the anterior PSM during somitogenesis (Kondow et al, 2006). In bowline-knockdown embryos, somite boundary formation is disrupted, and the regions of mespb and X-delta-2 expression, which are restricted to the anterior PSM during normal development, are expanded anteriorly .…”

Section: Introductionmentioning

confidence: 99%

“…Bowline/Ripply family proteins have two conserved domains: the WRPW tetrapeptide motif and the Ripply homology domain/Bowline-DSCR-Ledgerline conserved region (Ripply/BDLC domain). Through the WRPW-tetrapeptide motif, Xenopus Bowline and zebrafish Ripply1 interact with the transcriptional co-repressor Groucho/TLE (Kawamura et al, 2005;Kondow et al, 2006). We have previously shown that Bowline interacts with Tbx6 protein via the Ripply/BDLC domain and suppresses the transcriptional activity of Tbx6 .…”

Section: Introductionmentioning

confidence: 99%

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The Xenopus Bowline/Ripply family proteins negatively regulate the transcriptional activity of T-box transcription factors

et al. 2009

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Bowline, which is a member of the Xenopus Bowline/Ripply family of proteins, represses the transcription of somitogenesis-related genes before somite segmentation, which makes Bowline indispensable for somitogenesis. Although there are three bowline/Ripply family genes in each vertebrate species, it is not known whether the Bowline/Ripply family proteins share a common role in development. To elucidate their developmental roles, we examined the expression patterns and functions of the Xenopus Bowline/Ripply family proteins Bowline, Ledgerline, and a novel member of this protein family, xRipply3. We found that the expression patterns of bowline and ledgerline overlapped in the presomitic mesoderm (PSM), whereas ledgerline was additionally expressed in the newly formed somites. In addition, we isolated xRipply3, which is expressed in the pharyngeal region. Co-immunoprecipitation assays revealed that Ledgerline and xRipply3 interacted with T-box proteins and the transcriptional co-repressor Groucho/TLE. In luciferase assays, xRipply3 weakly suppressed the transcriptional activity of Tbx1, while Ledgerline strongly suppressed that of Tbx6. In line with the repressive role of Ledgerline, knockdown of Ledgerline resulted in enlargement of expression regions of the somitogenesis-related-genes mespb and Tbx6. Inhibition of histone deacetylase activity increased the expression of mespb, as seen in the Bowline and Ledgerline knockdown experiments. These results suggest that the Groucho-HDAC complex is required for the repressive activity of Bowline/Ripply family proteins during Xenopus somitogenesis. We conclude that although the Xenopus Bowline/Ripply family proteins Bowline, Ledgerline and xRipply3 are expressed differentially, they all act as negative regulators of T-box proteins.

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Mouse Ripply2 is downstream of Wnt3a and is dynamically expressed during somitogenesis

Biris

Dunty

Yamaguchi

2007

Developmental Dynamics

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Somites are blocks of mesoderm that form when segment boundaries are periodically generated in the anterior presomitic mesoderm (PSM). Periodicity is thought to be driven by an oscillating Notch-centered segmentation clock, whereas boundaries are spatially positioned by the secreted signaling molecules Wnt3a and Fgf8. We identified the putative transcriptional corepressor Ripply2 as a differentially expressed gene in wild-type and Wnt3a

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Bowline, a novel protein localized to the presomitic mesoderm, interacts with Groucho/TLE in Xenopus

Cited by 25 publications

References 43 publications

In vitro organogenesis from undifferentiated cells in Xenopus

In vitro organogenesis from undifferentiated cells in Xenopus

The Xenopus Bowline/Ripply family proteins negatively regulate the transcriptional activity of T-box transcription factors

Mouse Ripply2 is downstream of Wnt3a and is dynamically expressed during somitogenesis

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