Box-Behnken design in the development of optimized complex medium for phenol degradation using Pseudomonas putida (NICM 2174)

Annadurai, G.; Balan, S. Mathalai; Murugesan, Thanapalan

doi:10.1007/pl00009082

Cited by 19 publications

(19 citation statements)

References 22 publications

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“…However, most laboratory studies on phenol degradation have been carried out at an optimum temperature of 30°C (Annadurai et al 2002;Paraskevi and Euripides 2005). Annadurai et al (1999) and Chitra (1995) described that when the temperature increased to beyond 30°C or 34°C, no phenol degradation was observed due to cell decay, which is a temperature-dependent parameter.…”

Section: Effect Of Substrate Concentrationmentioning

confidence: 97%

“…Most organisms cannot tolerate pH values below 4.0 or above 9.0 (Kim and Armstrong 1981). At low (4.0) or high (9.0) pH values, acids or bases can penetrate into cells more easily, because they tend to exist in undissociated form under these conditions and electrostatic force cannot prevent them from entering cells (Robertson and Alexander 1992;Kim and Armstrong 1981;Chitra 1995;Annadurai et al 1999). …”

Section: Effect Of Phmentioning

confidence: 97%

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Isolation and characterisation of phenol-degrading Pseudomonas aeruginosa MTCC 4996

Kotresha

Vidyasagar

2007

World J Microbiol Biotechnol

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A novel indigenous Pseudomonas aeruginosa strain (MTCC 4996) isolated from a pulp industrial effluent-contaminated site was capable of degrading phenol up to a concentration of 1,300 mg L -1 within 156 h. Complete degradation was observed at pH values ranging from 6.0 to 10.0 and temperatures from 15 to 45°C, with an optimum pH of 7.0 and optimum temperature of 37°C. At an optimum shaking speed of 100-125 rpm, 100% degradation was observed in 66 h, as compared to 84 h under static conditions. Glucose and peptone at lower concentrations enhanced phenol degradation. The rate of phenol degradation was most sensitive to added Hg. Low concentrations of Fe, Cu, Pb, Zn, and Mn stimulated and enhanced the rate of degradation.

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Section: Effect Of Substrate Concentrationmentioning

confidence: 97%

Section: Effect Of Phmentioning

confidence: 97%

Isolation and characterisation of phenol-degrading Pseudomonas aeruginosa MTCC 4996

Kotresha

Vidyasagar

2007

World J Microbiol Biotechnol

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“…RSM with CCD reduces the load of experimental measurements, saves both time and cost [33,34], as well as minimizes errors in determining the effects of parameters It is also capable of determining interactive effects among the treatment variables [31,35].…”

Section: Introductionmentioning

confidence: 99%

Photo-fermentational hydrogen production of Rhodobacter sp. KKU-PS1 isolated from an UASB reactor

Assawamongkholsiri

Reungsang

2015

Electronic Journal of Biotechnology

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Background: In this study, the detection of nifH and nifD by a polymerase chain reaction assay was used to screen the potential photosynthetic bacteria capable of producing hydrogen from five different environmental sources. Efficiency of photo-hydrogen production is highly dependent on the culture conditions. Initial pH, temperature and illumination intensity were optimized for maximal hydrogen production using response surface methodology with central composite design. Results: Rhodobacter sp. KKU-PS1 (GenBank Accession No. KC478552) was isolated from the methane fermentation broth of an UASB reactor. Malic acid was the favored carbon source while Na-glutamate was the best nitrogen source. The optimum conditions for simultaneously maximizing the cumulative hydrogen production (H max ) and hydrogen production rate (R m ) from malic acid were an initial of pH 7.0, a temperature of 25.6°C, and an illumination intensity of 2500 lx. H max and R m levels of 1264 ml H 2 /l and 6.8 ml H 2 /L-h were obtained, respectively. The optimum initial pH and temperature were further used to optimize the illumination intensity for hydrogen production. An illumination intensity of 7500 lx gave the highest values of H max (1339 ml H 2 /l) and R m (12.0 ml H 2 /L-h) with a hydrogen yield and substrate conversion efficiency of 3.88 mol H 2 /mol malate and 64.7%, respectively. Conclusions: KKU-PS1 can produce hydrogen from at least 8 types of organic acids. By optimizing pH and temperature, a maximal hydrogen production by this strain was obtained. Additionally, by optimizing the light intensity, R m was increased by approximately two fold and the lag phase of hydrogen production was shortened.

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“…Of particular interest, BBD has been widely used for medium optimisation for different microbial systems (Annadurai et al, 1999;Ramnani and Gupta, 2004;Bae and Shoda, 2005). However, information regarding the application of this approach with microalgae is scarce in the literature.…”

mentioning

confidence: 99%

Optimising the lipid production of the green alga Neochloris oleoabundans using box–behnken experimental design

Wang

Lan

2011

Can J Chem Eng

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A systematic approach involving factor grouping, factorial design and response surface analysis was demonstrated to be an effective method for studying the effects of a large number of nutrients on cell growth and lipid production of the green alga Neochloris oleoabundans for the purpose of medium optimisation. Box-Behnken experimental design (BBD) was used for factorial experimental design and response surface method (RSM) was used for data analysis. The optimised medium obtained was able to support a lipid yield of 641.4 mg lipid/L and a cell density of 2.54 g/L, which were 4.5-and 2.2-fold of that obtained with the basic medium, modified Bristol medium (MBM), respectively.Une approche systématique impliquant un groupement de facteur, un plan factoriel et une analyse de surface de réponse s'est démontréeêtre une méthode efficace pour l'étude des effets d'un grand nombre d'éléments nutritifs sur la croissance cellulaire et la production de lipides de l'algue verte Neochloris oleoabundans, aux fins d'optimisation des milieux de culture. Le concept expérimental Box-Behnken (BBD) aété utilisé pour le plan expérimental factoriel et la méthode de surface de réponse (RSM) aété utilisée pour l'analyse des données. Le milieu optimisé obtenu aété en mesure de soutenir une production de lipides de 641,4 mg de lipides/L et une densité cellulaire de 2,54 g/L, ce qui représente des valeurs 4,5 fois et 2,2 fois supérieuresà celles obtenues avec le milieu de base, le milieu Bristol modifié (MBM), respectivement.

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Box-Behnken design in the development of optimized complex medium for phenol degradation using Pseudomonas putida (NICM 2174)

Cited by 19 publications

References 22 publications

Isolation and characterisation of phenol-degrading Pseudomonas aeruginosa MTCC 4996

Isolation and characterisation of phenol-degrading Pseudomonas aeruginosa MTCC 4996

Photo-fermentational hydrogen production of Rhodobacter sp. KKU-PS1 isolated from an UASB reactor

Optimising the lipid production of the green alga Neochloris oleoabundans using box–behnken experimental design

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