Bradyrhizobium canariense sp. nov., an acid-tolerant endosymbiont that nodulates endemic genistoid legumes (Papilionoideae: Genisteae) from the Canary Islands, along with Bradyrhizobium japonicum bv. genistearum, Bradyrhizobium genospecies alpha and Bradyrhizobium genospecies beta

Vinuesa, Pablo; León-Barrios, Milagros; Silva, Claudia; Willems, Anne; Jarabo-Lorenzo, A.; Pérez-Galdona, Ricardo; Werner, Dietrich; Martı́nez-Romero, Esperanza

doi:10.1099/ijs.0.63292-0

Cited by 253 publications

(159 citation statements)

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“…Since B. yuanmingense was found nodulating cowpea in Africa, our findings are confirmation of this point and further extend the geographical distribution of this species in Japan. A non-human-mediated wide geographical distribution has been also reported previously for B. canariense (32) and Mesorhizobium plurifarium (34).…”

Section: Discussionmentioning

confidence: 84%

“…According to microbe-host specificity, rhizobia isolated from cowpea are generally placed in the cowpea-crossinoculated group (1) and species of this heterogeneous group were assigned, based on phylogenetics, to the genus Bradyrhizobium (13). Created in 1982 (12), the genus Bradyrhizobium now includes seven species: Bradyrhizobium japonicum (12), Bradyrhizobium elkanii (17), Bradyrhizobium liaoningense (36), Bradyrhizobium yuanmingense (37), Bradyrhizobium betae (21), Bradyrhizobium canariense (32), and Bradyrhizobium denitrificans (30). Despite this number, however, studies on cowpea bradyrhizobia are limited.…”

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confidence: 99%

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Phylogenetic Diversity and Symbiotic Effectiveness of Root-Nodulating Bacteria Associated with Cowpea in the South-West Area of Japan

et al. 2009

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The phylogenetic diversity of cowpea root-nodulating bacteria in the South-West of Japan was investigated using 60 isolates. Seeds of cowpea were aseptically sown in vermiculite and inoculated with a suspension of Cowpea Soil (CS) or Bean Soil (BS) or without a soil suspension as a control. CS and BS were collected from the Kyushu University's farm (Japan) at sites where cowpea and bean, respectively, have been cultivated previously. Based on an analysis of the 16S rRNA gene and the Internal Transcribed Spacer (ITS) sequence between the 16S and 23S rRNA genes, 56 isolates were assigned to the genus Bradyrhizobium, while one isolate was found to be closely related to the genus Ralstonia. The ITS-based phylogeny showed 53 isolates, 2 isolates, and 1 isolate, to be closely related to B. yuanmingense, B. elkanii and B. japonicum, respectively, suggesting that B. yuanmingense strains predominated in the soils. Among the isolates tested, B. yuanmingense TSC10 and TTC9 exhibited a greater symbiotic activity and could be considered efficient inoculants for cowpea.

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Section: Discussionmentioning

confidence: 84%

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confidence: 99%

Phylogenetic Diversity and Symbiotic Effectiveness of Root-Nodulating Bacteria Associated with Cowpea in the South-West Area of Japan

et al. 2009

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“…The ITS and maintenance genes are currently used as markers for molecular systematics and for estimations of phylogenetic relationships among bradyrhizobia [24][25][26][27][28] , with the 16S rRNA gene having few polymorphisms within the Bradyrhizobium genus 25,27,28 [29][30][31] . In the present study, B. yuanmingense strains were isolated from the root nodule of soya bean from Yangon Region and Shan State.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic diversity of indigenous soya bean bradyrhizobia from different agro-climatic regions in Myanmar

Soe¹,

Yamakawa²,

Hashimoto³

et al. 2013

ScienceAsia

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Soya bean (Glycine max Merr.) is the most important legume crop in the world and an important cash crop in Myanmar. In the present study, we characterized 43 isolates of indigenous soya bean rhizobia from Myanmar based on the sequence analysis of the bacterial 16S-23S rRNA internal transcribed spacer region. The sequence analysis confirmed that all isolates were categorized and identified as the genus Bradyrhizobium and they were conspecific with B. japonicum, B. elkanii, B. yuanmingense, and Bradyrhizobium sp. The highest genetic diversity of Bradyrhizobium strains was observed in the Shan State soya bean growing area. B. elkanii was distributed throughout the cultivation areas and was recorded as the dominant soya bean-nodulating strain in Myanmar. The present study revealed that there were two Rj-genotypes as Rj4-and non-Rj-genotypes among the collected soya bean cultivars from Myanmar. These findings indicate a high compatibility between Rj4-genotypes soya beans and nodulation type B strains in Shan State and Yangon region. In Mandalay region, the compatibility between non-Rj-genotype soya beans and nodulation type A strains was clearly investigated. This is the first report describing B. yuanmingense from nodules of soya bean and the geographical distribution of indigenous bradyrhizobia of soya bean from different regions in Myanmar.

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“…The analysis of a small number of carefully selected gene sequences may equal or even surpass the precision of DNA-DNA hybridization for the quantification of genome relatedness and this approach thus has the potential to replace cumbersome DNA-DNA hybridizations (Zeigler, 2003, Martens et al, 2008. Mantelin et al (2006) included MLSA in the description of four novel species of the genus Phyllobacterium and Vinuesa et al (2005c) described novel species of the genus Bradyrhizobium using phylogenetic analysis of three housekeeping genes, atpD, glnII and recA, combined with other classical genotypic and phenotypic analyses. 'Ensifer mexicanus' and 'Sinorhizobium chiapanecum' were described using phenotypic analysis, phylogenies of the recA, gyrA, nolR, rpoB, rrs and symbiotic genes and confidence intervals of sequence similarity to estimate both inter-and intraspecies variation; all were in correlation with DNA-DNA hybridization data (Lloret et al, 2007;Rincó nRosales et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Multilocus sequence analysis of root nodule isolates from Lotus arabicus (Senegal), Lotus creticus, Argyrolobium uniflorum and Medicago sativa (Tunisia) and description of Ensifer numidicus sp. nov. and Ensifer garamanticus sp. nov.

Merabet

Martens

Mahdhi

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

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Nine isolates from Argyrolobium uniflorum, Lotus creticus, Medicago sativa (Tunisia) and Lotus arabicus (Senegal) were analysed by multilocus sequence analysis (MLSA) of five housekeeping genes (recA, atpD, glnA, gltA and thrC), the 16S rRNA gene and the nodulation gene nodA. Analysis of the individual and concatenated gene sequences demonstrated that the nine new strains constituted three stable, well-supported (bootstrap and gene sequence similarity values) monophyletic clusters, A, B and C, all belonging to the branch of the genus Ensifer, regardless of the phylogenetic reconstruction method used (maximum likelihood, maximum-parsimony, neighbour-joining). The three groups were further characterized by API 100 auxanographic tests, host specificity and nodA gene sequence analysis. On the basis of these data, clusters A and C are suggested as representing two novel species within the genus Ensifer, for which the names Ensifer numidicus sp. nov. (type strain ORS 1407T=LMG 24690T=CIP 109850T) and Ensifer garamanticus sp. nov. (type strain ORS 1400T=LMG 24692T=CIP 109916T) are proposed. The cluster B strains were assigned to Ensifer adhaerens genomovar A.

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Bradyrhizobium canariense sp. nov., an acid-tolerant endosymbiont that nodulates endemic genistoid legumes (Papilionoideae: Genisteae) from the Canary Islands, along with Bradyrhizobium japonicum bv. genistearum, Bradyrhizobium genospecies alpha and Bradyrhizobium genospecies beta

Abstract: Bradyrhizobium canariense sp. nov., an acidtolerant endosymbiont that nodulates endemic genistoid legumes (Papilionoideae: Genisteae) from the Canary Islands, along with Bradyrhizobium japonicum bv. genistearum, Bradyrhizobium genospecies alpha and Bradyrhizobium genospecies beta

Cited by 253 publications

References 41 publications

Phylogenetic Diversity and Symbiotic Effectiveness of Root-Nodulating Bacteria Associated with Cowpea in the South-West Area of Japan

Phylogenetic Diversity and Symbiotic Effectiveness of Root-Nodulating Bacteria Associated with Cowpea in the South-West Area of Japan

Phylogenetic diversity of indigenous soya bean bradyrhizobia from different agro-climatic regions in Myanmar

Multilocus sequence analysis of root nodule isolates from Lotus arabicus (Senegal), Lotus creticus, Argyrolobium uniflorum and Medicago sativa (Tunisia) and description of Ensifer numidicus sp. nov. and Ensifer garamanticus sp. nov.

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