Brain metabolomics study for the protective effects of <i>Rhodiola crenulata</i> extract on Alzheimer's disease by HPLC coupled with Fourier transform‐ion cyclotron resonance mass spectrometry

Li, Xintong; Wang, Yanan; Su, Mingming; Chu, Xiaowen; Li, Siqi; Yue, Yiqiang; Zhang, Xiaoxue; Wang, Jiahong; Han, Fei

doi:10.1002/jssc.201901314

Cited by 7 publications

(7 citation statements)

References 36 publications

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“…FT-ICR MS can determine the mass-to-charge ratio (m/z) of ions by detecting the frequency of their cyclotron motion in a high magnetic field and can use to analyze complex components, due to their high resolution and quality accuracy [27]. In recent years, it has been applied to decisively identify the chemical constituents by accurate mass and isotopic peaks in many TCMs, such as Cortex Fraxini, Rhodiola crenulata, Gansuibanxia decoction, and so on [28][29][30]. Therefore, HPLC-FT-ICR MS is a suitable method for the detection and characterization of chemical constituents in TCMs.…”

Section: Introductionmentioning

confidence: 99%

Studies on chemical constituents of Flos Puerariae‐Semen Hoveniae medicine pair by HPLC and Fourier transform ion cyclotron resonance mass spectrometry

Wei

et al. 2021

J of Separation Science

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Alcoholic liver disease is currently the most clinically concerning liver disease, which occurs from chronic alcohol abuse. Flos Puerariae and Semen Hoveniae have been used to treat alcohol drinking excessively for thousands of years in China. In this study, the ethanol extract of the medicine pair was qualitatively and quantitatively analyzed by high-performance liquid chromatography and Fourier transform ion cyclotron resonance mass spectrometry. First, the high-performance liquid chromatography fingerprint was established to obtain the overall chromatographic data of its chemical constituents. Next, highperformance liquid chromatography-mass spectrometry was applied to identify its chemical constituents. Then, the characteristic constituents were simultaneously quantified by high-performance liquid chromatography. In addition, the chemical constituents that were absorbed into rat plasma were identified by high-performance liquid chromatography-mass spectrometry. As a result, a total of 48 chemical constituents in the medicine pair were detected and identified in vitro. Meanwhile, the content of seven representative constituents, including dihydromyricetin, glycitin, genistin, tectoridin, glycitein, genistein, and tectorigenin were simultaneously determined. Furthermore, a total of 19 chemical constituents were detected in rat plasma after oral administration. In short, the chemical constituents of the medicine pair were initially investigated in this study, which will lay the foundation for the discovery of its pharmacodynamic substances in further works.

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Section: Introductionmentioning

confidence: 99%

Studies on chemical constituents of Flos Puerariae‐Semen Hoveniae medicine pair by HPLC and Fourier transform ion cyclotron resonance mass spectrometry

Wei

et al. 2021

J of Separation Science

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“…However, these single-target drugs are not only associated with various adverse effects and toxicity but also less effective in reversing the pathological progression of AD [ 29 , 30 ]. By contrast, because of their multitarget and multichannel properties, medicinal herbs and their derived ingredients (MHDIs) demonstrate considerably exceptional AD treatment outcomes compared with the aforementioned drugs [ 11 , 31 ]. Therefore, developing potential MHDI-based AD treatment strategies is highly warranted.…”

Section: Introductionmentioning

confidence: 99%

Medicinal Herbs and Their Derived Ingredients Protect against Cognitive Decline in In Vivo Models of Alzheimer’s Disease

Tsai

Kao

Cheng

2022

IJMS

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Alzheimer’s disease (AD) has pathological hallmarks including amyloid beta (Aβ) plaque formation. Currently approved single-target drugs cannot effectively ameliorate AD. Medicinal herbs and their derived ingredients (MHDIs) have multitarget and multichannel properties, engendering exceptional AD treatment outcomes. This review delineates how in in vivo models MHDIs suppress Aβ deposition by downregulating β- and γ-secretase activities; inhibit oxidative stress by enhancing the antioxidant activities and reducing lipid peroxidation; prevent tau hyperphosphorylation by upregulating protein phosphatase 2A expression and downregulating glycogen synthase kinase-3β expression; reduce inflammatory mediators partly by upregulating brain-derived neurotrophic factor/extracellular signal-regulated protein kinase 1/2-mediated signaling and downregulating p38 mitogen-activated protein kinase (p38 MAPK)/c-Jun N-terminal kinase (JNK)-mediated signaling; attenuate synaptic dysfunction by increasing presynaptic protein, postsynaptic protein, and acetylcholine levels and preventing acetylcholinesterase activity; and protect against neuronal apoptosis mainly by upregulating Akt/cyclic AMP response element-binding protein/B-cell lymphoma 2 (Bcl-2)-mediated anti-apoptotic signaling and downregulating p38 MAPK/JNK/Bcl-2-associated x protein (Bax)/caspase-3-, Bax/apoptosis-inducing factor-, C/EBP homologous protein/glucose-regulated protein 78-, and autophagy-mediated apoptotic signaling. Therefore, MHDIs listed in this review protect against Aβ-induced cognitive decline by inhibiting Aβ accumulation, oxidative stress, tau hyperphosphorylation, inflammation, synaptic damage, and neuronal apoptosis in the cortex and hippocampus during the early and late AD phases.

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“…The patients show cognitive dysfunction, gradual memory decline, behavior abnormality, and social disorder [3], and cannot take care of themselves when the symptoms become severe, which brings great burden to the family and society. Therefore, it is crucial that find the means to alleviate the symptoms of the disease and to slow its progression [4].…”

Section: Introductionmentioning

confidence: 99%

Mass spectrometry‐based urinary metabolomics for exploring the treatment effects of Radix ginseng‐Schisandra chinensis herb pair on Alzheimer's disease in rats

Wang

Liu

et al. 2021

J of Separation Science

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Herb pairs are the unique combinations of two relatively fixed herbs, intrinsically convey the basic idea of traditional Chinese medicine prescriptions. The compatibility of Radix ginseng and Schisandra chinensis has been used in traditional Chinese medicine for treating Alzheimer's disease for many years. However, there are few studies on Radix ginseng‐Schisandra chinensis herb pair, and the underlying action mechanism is still unclear. In this study, the mechanism of Radix ginseng‐Schisandra chinensis herb pair on Alzheimer's disease was investigated by using the mass spectrometry‐based urinary metabolomics method. Sixteen urinary endogenous metabolites were identified as potential biomarkers. Meanwhile, 10 biomarkers were quantified with tandem mass spectrometry. The study result showed that the brain pathologic symptoms of model rats were improved and the potential biomarkers were adjusted backward significantly after the herb pair administration. The metabolic pathways linked to the herb pair‐regulated endogenous biomarkers included phenylalanine and tyrosine metabolism, tryptophan metabolism, purine metabolism, and so on. The above metabolic pathways reflected that Radix ginseng‐Schisandra chinensis herb pair mainly regulates abnormal energy metabolism, reduces inflammation, and regulates gut microbiota and neurotransmitters in the treatment of Alzheimer's disease.

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Brain metabolomics study for the protective effects of Rhodiola crenulata extract on Alzheimer's disease by HPLC coupled with Fourier transform‐ion cyclotron resonance mass spectrometry

Cited by 7 publications

References 36 publications

Studies on chemical constituents of Flos Puerariae‐Semen Hoveniae medicine pair by HPLC and Fourier transform ion cyclotron resonance mass spectrometry

Studies on chemical constituents of Flos Puerariae‐Semen Hoveniae medicine pair by HPLC and Fourier transform ion cyclotron resonance mass spectrometry

Medicinal Herbs and Their Derived Ingredients Protect against Cognitive Decline in In Vivo Models of Alzheimer’s Disease

Mass spectrometry‐based urinary metabolomics for exploring the treatment effects of Radix ginseng‐Schisandra chinensis herb pair on Alzheimer's disease in rats

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