“…Human carcinoma cell lines BÜX [7], OHS [8], FEMX [9], EKVX [10], PC-MM1 (kindly provided by Klaus Pantel, Germany), OSA (kindly provided by Dr. A Thomas Look, St Jude’s hospital, Memphis, USA), SF-295 and U-138MG [11], MA11 [12] and D54MG [13] were cultured in RPMI-1640 medium (BioWhittaker, Verviers, Belgium) supplemented with 10% FCS (Gibco BRL, Paisley, UK), 2 mM l -glutamine (BioWhittaker, Verviers, Belgium) and 10 mM HEPES (BioWhittaker, Verviers, Belgium). Other cell lines, BT-474, T-47D, MCF-7, MDA-MB-231, SK-BR-3, HT-29, HCT 116, HTB-182, H146, SK-MEL-28, SW900, HeLa, OVCAR-3, SKOV-3, DU145, LNCaP and LL 86 were obtained from The American Type Culture Collection (ATCC) and cultivated as described above.…”