Brain schistosomiasis in mice experimentally infected with Schistosoma mansoni

Lambertucci, José Roberto; Fidelis, Thiago Andre Alves; Pereira, Thiago A.; Coelho, Paulo Marcos Zech; Araujo, Nathanniely Deyse de; Souza, Márcia María de; Filho, Geraldo Brasileiro; Pereira, Fausto Edmundo Lima; Antunes, Carlos Maurício de Figueiredo

doi:10.1590/0037-8682-0083-2013

Cited by 9 publications

(10 citation statements)

References 14 publications

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“…Surprisingly, S. mansoni eggs and granulomas were identified in the central nervous system of symptomatic animals (Lambertucci et al 2014). Central nervous system involvement can occur during acute primary infections (Chitsulo et al 2000;Amaral et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Impact of gold nanoparticles on brain of mice infected with Schistosoma mansoni

et al. 2015

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Schistosomiasis is a condition characterized by high rates of morbidity and cognitive impairment. It afflicts many people in tropical and sub-tropical countries. Our study aimed to investigate the protective role of gold nanoparticles (GNPs) on the brain of mice infected with Schistosoma mansoni. Characterizations of GNPs were determined by using high-resolution transmission electron microscopy. Three doses of GNPs (0.25, 0.5, and 1.0 mg/kg body weight) were used to treat animals after S. mansoni infection. The infection induced impairments in histological picture as a result of schistosome infection resulting in a disturbance in the content of the brain neurotransmitters, norepinephrine (NE), and dopamine (DA). Also, the infection induced significant reduction in glutathione level; oppositely, the levels of nitric oxide and malondialdehyde were increased significantly. In addition, S. mansoni was able to disregulate the infected mice brain Cacnb4, Cabp4, Vdac3, Glrb, and Adam23 messenger RNA (mRNA). On the other hand, treatment of mice with GNPs could alleviate the histological impairments, the changes in the content of NE and DA, and the brain oxidative damage. Also, GNPs could regulate the gene expression due to S. mansoni infection. Generally, GNPs could decrease the neurooxidative stress and regulated the gene expression in the brain of infected mice. Consequently, our results revealed an anti-neuroschistosomal effect of GNPs in mice infected with S. mansoni.

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Section: Introductionmentioning

confidence: 99%

Impact of gold nanoparticles on brain of mice infected with Schistosoma mansoni

et al. 2015

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“…and that 800 million people in the world are at risk of infection. In Brazil, approximately 2.5 million people are infected with Schistosoma mansoni and 30 million are exposed to infection [1,2].…”

Section: Introductionmentioning

confidence: 99%

“…Central Nervous System (CNS) involvement in schistosomiasis can occur during acute primary infections, but as the disease becomes chronic, neurological complications can occur as the newly forming of granulomas are smaller, shrunken and fibrotic [2,3].…”

Section: Introductionmentioning

confidence: 99%

“…Alves (1958) found that in 28% of 150 unselected autopsy cases S. haematobium eggs were detected in the brain [9][10][11]. It is estimated that the incidence of neurological complications varies between 0.3% and 4% of schistosomiasis mansoni [2]. The incidence of encephalic demage caused by S. mansoni in humans is unknown.…”

Section: Introductionmentioning

confidence: 99%

“…Neurological manifestations of schistosomiasis are caused by an increase in intracranial pressure, and the focal signs are triggered by the tumor masses produced by granulomas, often in the productive phase with slight fibrosis, which suggested the chronic phase of infection. The initial signs and symptoms include headache, focal or generalized seizures, ataxia, nystagmus, nausea and vomiting, intracranial hypertension and various neurological deficits [2]. For many years, we have observed evidence of brain disease (hemiplegia, spinning and urinary retention) in mice infected with S. mansoni, but these mice were considered to have other diseases, such as labyrinthitis or cerebral infection [2].…”

Section: Introductionmentioning

confidence: 99%

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Murine Model of Neuroschistosomiasis Mansoni: Clinical, Histological and Magnetic Resonance Imaging Studies

Fidelis¹,

Parreiras²,

Moll³

et al. 2018

Adv Tech Biol Med

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The schistosomiasis mansoni infection is responsible for 3.6% of the worldwide estimated causes of death and the central nervous system can be affected. In humans, the eggs of this helminth have been found in the leptomeninges, cerebral cortex, basal ganglia, choroid plexus, cerebellum and spinal cord. Neurological manifestations, histhology and magnetic resonance imaging of neuroschistosomiasis mansoni in humans serve as our chief reference points for the examination of the experimental infections in murine model. In this study, experimental infection of S. mansoni cercariae in mice aims to demonstrate the presence of granulomas in the brain and correlate to the clinical, histologic, and magnetic resonance findings. Twenty five Swiss-webster mice were infected subcutaneously, and followed for 160 days post-infection. Another group of twenty five mice were not infected and kept as controls. Images were obtained in the different planes by magnetic resonance. Histological samples were stained by Hematoxilin and Eosin (HE) to examine S. mansoni eggs, granulomas and inflammatory lesions. The results showed neurological manifestations as head and chest tilt (to the left or right side), hemiparesis, ataxia, body contortion, loss of balance and spinning, induced by granulomas in several regions of the central nervous system, and vascular changes associated with haemorrages. The MRI indicated multiple irregular nodules dispersed associated with oedema. These findings indicate that the murine model subcutaneously infected by S. mansoni cercarie may be used for studying mechanisms leading to human neuroschistosomiasis.

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