Brain tumour stem cells

Vescovi, Angelo L.; Galli, Rossella

doi:10.1038/nrc1889

Cited by 898 publications

(737 citation statements)

References 115 publications

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“…Reactivation of a subset of signature genes expressed in HSCs correlates with regaining of an enhanced self-renewal capacity [52]. Researches in brain tumor development also indicate that more committed neural progenitor cells are likely to be the targets of tumorigenic mutations [53].…”

Section: Origins Of Cscsmentioning

confidence: 99%

Beyond tumorigenesis: cancer stem cells in metastasis

et al. 2006

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Section: Origins Of Cscsmentioning

confidence: 99%

Beyond tumorigenesis: cancer stem cells in metastasis

et al. 2006

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“…As tumorigenesis is closely associated with abnormal gene expression and/or alternative splicing of pro-oncogenes [29][30][31] , the representative pro-oncogenes in the CNS were quantified 32,33 . We observed significant expressions of such pro-oncogenes in NSPCs and differences between the two in Igf2r, pdgfrb, Hif1a, Fos, Smo, Idh1, Pax6 and Olig2 (Fig.…”

Section: Differences In Nspcs Engrafted In Naïve and Injured Spinal Cmentioning

confidence: 99%

Direct isolation and RNA-seq reveal environment-dependent properties of engrafted neural stem/progenitor cells

et al. 2012

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neural stem/progenitor cell (nsPC) transplantation is a promising treatment for various neurodegenerative disorders including spinal cord injury, however, no direct analysis has ever been performed on their in vivo profile after transplantation. Here we combined bioimaging, flow-cytometric isolation and ultra-high-throughput RnA sequencing to evaluate the cellular properties of engrafted nsPCs. The acutely transplanted nsPCs had beneficial effects on spinal cord injury, particularly neuroprotection and neurohumoral secretion, whereas their in situ secretory activity differed significantly from that predicted in vitro. The RnA-sequencing of engrafted nsPCs revealed dynamic expression/splicing changes in various genes involved in cellular functions and tumour development depending on graft environments. notably, in the pathological environment, overall transcriptional activity, external signal transduction and neural differentiation of engrafted nsPCs were significantly suppressed. These results highlight the vulnerability of engrafted nsPCs to environmental force, while emphasizing the importance of in situ analysis in advancing the efficacy and safety of stem cell-based therapies.

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“…GSCs are thought to carry tumour initiation potential and play essential roles in tumour propagation, repopulation, therapeutic resistance and relapse. While capable of self-renewal, these cells retain the ability to activate cellular programmes that result in generation of rapidly dividing, committed and differentiated bulk GBM cell populations [11,12]. GSC maintenance and fate rely upon external regulatory interactions with the brain microenvironment, of which vascular niche is thought to play a critical role [13].…”

Section: Introductionmentioning

confidence: 99%

Molecular subtypes and differentiation programmes of glioma stem cells as determinants of extracellular vesicle profiles and endothelial cell‐stimulating activities

Spinelli

Montermini

Meehan

et al. 2018

J of Extracellular Vesicle

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We have previously uncovered the impact of oncogenic and differentiation processes on extracellular vesicles (EVs) in cancer. This is of interested in the context of glioma stem cells (GSC) that are responsible for recurrent nature of glioblastoma multiforme (GBM), while retaining the potential to undergo differentiation and self renewal. GSCs reside in vascular niches where they interact with endothelial cells through a number of mediators including bioactive cargo of EVs. GSCs can be classified as proneural (PN) or mesenchymal (MES) subtypes on the basis of their gene expression profiles and distinct biological characteristics. In the present study we investigated how GSC diversity and differentiation programmes influence their EV-mediated communication potentials. Indeed, molecular subtypes of GBMs and GSCs differ with respect to their expression of EV-related genes (vesiculome) and GSCs with PN or MES phenotypes produce EVs with markedly different characteristics, marker profiles, proteomes and endothelial stimulating activities. For example, while EVs of PN GSC are largely devoid of exosomal markers their counterparts from MES GSCs express ample CD9, CD63 and CD81 tetraspanins. In both GSC subtypes serum-induced differentiation results in profound, but distinct changes of cellular phenotypes including the enhanced EV production, reconfiguration of their proteomes and the related functional pathways. Notably, the EV uptake was a function of both subtype and differentiation state of donor cells. Thus, while, EVs produced by differentiated MES GSCs were internalized less efficiently than those from undifferentiated cells they exhibited an increased stimulatory potential for human brain endothelial cells. Such stimulating activity was also observed for EVs derived from differentiated PN GSCs, despite their even weaker uptake by endothelial cells. These findings suggest that the role of EVs as biological mediators and biomarkers in GBM may depend on the molecular subtype and functional state of donor cancer cells, including cancer stem cells.Abbreviations: CryoTEM: cryo-transmission electron microscopy; DIFF: differentiated GSCs; EGF: epidermal growth factor; DUC: differential ultracentrifugation; EV: extracellular vesicle; FGF: fibroblast growth factor; GBM: glioblastoma multiforme; GFAP: glial fibrillary acidic protein; GO: gene ontology; GSC: glioma stem cells; HBEC-5i: human brain endothelial cells; MES: mesenchymal cells; MTS - [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt; PMT1: proneural-to-mesenchyman transition cell line 1; PN: proneural cells; TEM: transmission electron microscopy; WB: western blotting

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Brain tumour stem cells

Cited by 898 publications

References 115 publications

Beyond tumorigenesis: cancer stem cells in metastasis

Beyond tumorigenesis: cancer stem cells in metastasis

Direct isolation and RNA-seq reveal environment-dependent properties of engrafted neural stem/progenitor cells

Molecular subtypes and differentiation programmes of glioma stem cells as determinants of extracellular vesicle profiles and endothelial cell‐stimulating activities

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