Branched-Chain Amino Acids

Pátek, Miroslav

doi:10.1007/7171_2006_070

Cited by 28 publications

(14 citation statements)

References 117 publications

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“…Further studies on the ACCase genes in barnyardgrass are needed to compare the differences among these genes, and determine their role in herbicide resistance. Penoxsulam and bispyribac-sodium herbicides, which were used in our study, were ALS-inhibiting herbicides [33]. In the barnyardgrass transcriptome and digital expression analysis, higher levels of ALS sequences (contig05544 and G81ESPA01CWAJO) were present in the resistant biotype than in the susceptible biotype after treatment with herbicides (Table 6).…”

Section: Resultsmentioning

confidence: 88%

De novo Assembly and Characterization of the Barnyardgrass (Echinochloa crus-galli) Transcriptome Using Next-Generation Pyrosequencing

Yang

2013

PLoS ONE

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BackgroundBarnyardgrass (Echinochloa crus-galli) is an important weed that is a menace to rice cultivation and production. Rapid evolution of herbicide resistance in this weed makes it one of the most difficult to manage using herbicides. Since genome-wide sequence data for barnyardgrass is limited, we sequenced the transcriptomes of susceptible and resistant barnyardgrass biotypes using the 454 GS-FLX platform.Results454 pyrosequencing generated 371,281 raw reads with an average length of 341.8 bp, which made a total length of 126.89 Mb (SRX160526). De novo assembly produced 10,142 contigs (∼5.92 Mb) with an average length of 583 bp and 68,940 singletons (∼22.13 Mb) with an average length of 321 bp. About 244,653 GO term assignments to the biological process, cellular component and molecular function categories were obtained. A total of 6,092 contigs and singletons with 2,515 enzyme commission numbers were assigned to 151 predicted KEGG metabolic pathways. Digital abundance analysis using Illumina sequencing identified 78,124 transcripts among susceptible, resistant, herbicide-treated susceptible and herbicide-treated resistant barnyardgrass biotypes. From these analyses, eight herbicide target-site gene groups and four non-target-site gene groups were identified in the resistant biotype. These could be potential candidate genes involved in the herbicide resistance of barnyardgrass and could be used for further functional genomics research. C4 photosynthesis genes including RbcS, RbcL, NADP-me and MDH with complete CDS were identified using PCR and RACE technology.ConclusionsThis is the first large-scale transcriptome sequencing of E. crus-galli performed using the 454 GS-FLX platform. Potential candidate genes involved in the evolution of herbicide resistance were identified from the assembled sequences. This transcriptome data may serve as a reference for further gene expression and functional genomics studies, and will facilitate the study of herbicide resistance at the molecular level in this species as well as other weeds.

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Section: Resultsmentioning

confidence: 88%

De novo Assembly and Characterization of the Barnyardgrass (Echinochloa crus-galli) Transcriptome Using Next-Generation Pyrosequencing

Yang

2013

PLoS ONE

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“…Regulation of the pathways includes multivalent feedback inhibition of the first common enzyme of the pathways, acetohydroxy acid synthase (AHAS), by all three amino acids, as well as multivalent regulation of gene expression by transcriptional attenuation (for a review, see Pátek, 2007).…”

Section: Introductionmentioning

confidence: 99%

Metabolic engineering of the L-valine biosynthesis pathway in Corynebacterium glutamicum using promoter activity modulation

Holátko

Elišáková

Prouza

et al. 2009

Journal of Biotechnology

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“…In microorganisms and in plants, 2-ketoisovalerate is synthesized from two molecules of pyruvate in a pathway comprising three reactions, catalyzed by acetohydroxyacid synthase (AHAS; ilvBN gene product), acetohydroxyacid isomeroreductase (AHAIR; ilvC gene product), and dihydroxyacid dehydratase (DHAD; ilvD gene product) (38) (Fig. 1).…”

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confidence: 99%

Metabolic Engineering of Corynebacterium glutamicum for 2-Ketoisovalerate Production

Krause

Blombach

Eikmanns

2010

Appl Environ Microbiol

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2-Ketoisovalerate is used as a therapeutic agent, and a 2-ketoisovalerate-producing organism may serve as a platform for products deriving from this 2-keto acid. We engineered the wild type of Corynebacterium glutamicum for the growth-decoupled production of 2-ketoisovalerate from glucose by deletion of the aceE gene encoding the E1p subunit of the pyruvate dehydrogenase complex, deletion of the transaminase B gene ilvE, and additional overexpression of the ilvBNCD genes, encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase (AHAS), acetohydroxyacid isomeroreductase, and dihydroxyacid dehydratase. 2-Ketoisovalerate production was further improved by deletion of the pyruvate:quinone oxidoreductase gene pqo. In fed-batch fermentations at high cell densities, the newly constructed strains produced up to 188 ؎ 28 mM (21.8 ؎ 3.2 g liter ؊1 ) 2-ketoisovalerate and showed a product yield of about 0.47 ؎ 0.05 mol per mol (0.3 ؎ 0.03 g per g) of glucose and a volumetric productivity of about 4.6 ؎ 0.6 mM (0.53 ؎ 0.07 g liter ؊1 ) 2-ketoisovalerate per h in the overall production phase. In studying the influence of the three branched-chain 2-keto acids 2-ketoisovalerate, 2-ketoisocaproate, and 2-keto-3-methylvalerate on the AHAS activity, we observed a competitive inhibition of the AHAS enzyme by 2-ketoisovalerate.

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Branched-Chain Amino Acids

Cited by 28 publications

References 117 publications

De novo Assembly and Characterization of the Barnyardgrass (Echinochloa crus-galli) Transcriptome Using Next-Generation Pyrosequencing

De novo Assembly and Characterization of the Barnyardgrass (Echinochloa crus-galli) Transcriptome Using Next-Generation Pyrosequencing

Metabolic engineering of the L-valine biosynthesis pathway in Corynebacterium glutamicum using promoter activity modulation

Metabolic Engineering of Corynebacterium glutamicum for 2-Ketoisovalerate Production

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