Brassinosteroids and Auxin Down-Regulate DELLA Genes in Fiber Initiation and Elongation of Cotton

Hu, Mingyu; Luo, Ming; Xiao, Yazhong; Li, Xianbi; Tan, Kunling; Hou, Lei; Dong, Jing; Li, De-Mou; Song, Shuiqing; Jun, Zhao; Zang, Zhenle; BaoLi, Li; Pei, Yan

doi:10.1016/s1671-2927(11)60107-7

Cited by 20 publications

(10 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further, the gene ontology (GO) analysis (Figure 7a) also revealed that the DEGs were enriched with development, cell wall, redox, hormone metabolism, lipid and secondary metabolismrelated genes. Many of these pathways are important for the fiber development in cotton (Hu et al, 2011;Zhang et al, 2011Zhang et al, , 2016aKim et al, 2012). The DEGs were further subjected to MapMan analysis to understand the precise pathways that were altered in the RNAi line.…”

Section: Rnai-mediated Suppression Of Ghhda5 Leads To Lower Fiber Inimentioning

confidence: 99%

Role of GhHDA5 in H3K9 deacetylation and fiber initiation in Gossypium hirsutum

Kumar

Singh

et al. 2018

The Plant Journal

View full text Add to dashboard Cite

Cotton fibers are single-celled trichomes that initiate from the epidermal cells of the ovules at or before anthesis. Here, we identified that the histone deacetylase (HDAC) activity is essential for proper cotton fiber initiation. We further identified 15 HDACs homoeologs in each of the A- and D-subgenomes of Gossypium hirsutum. Few of these HDAC homoeologs expressed preferentially during the early stages of fiber development [-1, 0 and 6 days post-anthesis (DPA)]. Among them, GhHDA5 expressed significantly at the time of fiber initiation (-1 and 0 DPA). The in vitro assay for HDAC activity indicated that GhHDA5 primarily deacetylates H3K9 acetylation marks. Moreover, the reduced expression of GhHDA5 also suppresses fiber initiation and lint yield in the RNA interference (RNAi) lines. The 0 DPA ovules of GhHDA5RNAi lines also showed alterations in reactive oxygen species homeostasis and elevated autophagic cell death in the developing fibers. The differentially expressed genes (DEGs) identified through RNA-seq of RNAi line (DEP12) and their pathway analysis showed that GhHDA5 modulates expression of many stress and development-related genes involved in fiber development. The reduced expression of GhHDA5 in the RNAi lines also resulted in H3K9 hyper-acetylation on the promoter region of few DEGs assessed by chromatin immunoprecipitation assay. The positively co-expressed genes with GhHDA5 showed cumulative higher expression during fiber initiation, and gene ontology annotation suggests their involvement in fiber development. Furthermore, the predicted protein interaction network in the positively co-expressed genes indicates HDA5 modulates fiber initiation-specific gene expression through a complex involving reported repressors.

show abstract

Section: Rnai-mediated Suppression Of Ghhda5 Leads To Lower Fiber Inimentioning

confidence: 99%

Role of GhHDA5 in H3K9 deacetylation and fiber initiation in Gossypium hirsutum

Kumar

Singh

et al. 2018

The Plant Journal

View full text Add to dashboard Cite

show abstract

“…These data suggest that BRs may attenuate the GAs-induced defense responses in rice through interference with GA signaling 60 . In contrast to the positive role of BRs in stabilizing DELLA protein in rice, BRs were found to reduce the expression of four DELLA genes in cotton ( Gossypium hirsutum ) fiber cells, including GhGAI1 , which is engaged in fiber cell initiation 63 . In a chromatin immunoprecipitation (ChIP) study in Arabidopsis , four of the five DELLA-encoding genes ( RGA , GAI , RGL1 and RGL3 ) were identified as direct targets of BZR1, the transcription factor in the BR pathway, suggesting that BRs may modulate GA responses by directly controlling the expression of DELLA-encoding genes 29 .…”

Section: Regulation Of the Expression Of Br And Ga Signaling Genesmentioning

confidence: 91%

“… 60 In contrast to the positive role of BRs in stabilizing DELLA protein in rice, BRs were found to reduce the expression of four DELLA genes in cotton ( Gossypium hirsutum ) fiber cells, including GhGAI1 , which is engaged in fiber cell initiation. 63 In a chromatin immunoprecipitation (ChIP) study in Arabidopsis , four of the five DELLA-encoding genes ( RGA , GAI , RGL1 and RGL3 ) were identified as direct targets of BZR1, the transcription factor in the BR pathway, suggesting that BRs may modulate GA responses by directly controlling the expression of DELLA-encoding genes. 29 However, in a recent study 9 we demonstrated by qualitative real-time PCR (qRT-PCR) analysis 9 that the expression of these DELLA genes was not affected by either BR treatment or by the bzr1-1D mutation that enhances BR signaling, suggesting that BZR1 and DELLAs coordinate the interaction between BRs and GAs in controlling cell elongation primarily through protein-protein interaction rather than through BZR1 regulation of DELLA gene expression.…”

Section: Regulation Of the Expression Of Br And Ga Signaling Genesmentioning

confidence: 99%

Mechanisms of signaling crosstalk between brassinosteroids and gibberellins

Li¹,

2013

Plant Signaling & Behavior

View full text Add to dashboard Cite

Brassinosteroids (BRs) and Gibberellins (GAs) are two principal groups of growth-promoting phytohormones. Accumulating evidence supports that there are crosstalks between BR and GA signaling pathways. However, a molecular mechanism for direct signaling crosstalk between BRs and GAs was not revealed until recently. Works from three different groups demonstrated that an interaction between BZR1/BES1 and DELLAs, two groups of key transcriptional regulators from the BR and GA signaling pathways, respectively, mediates the direct signaling crosstalk between BRs and GAs in controlling cell elongation in Arabidopsis. It was shown that DELLA proteins not only affect the protein stability but also inhibit the transcriptional activity of BZR1. Thus, GAs promote cell elongation, at least in part, through releasing DELLA-mediated inhibition of BZR1. This review aims to introduce these recent advances in our understanding of how BRs and GAs coordinate to regulate plant growth and development at the molecular level.

show abstract

“…Moreover, GA inhibits BR signaling as well as its biosynthesis in a feedback inhibiting loop but facilitate cell elongation through activating primary BR signaling pathway upon applying exogenous high GA concentration, indicating BR-GA crosstalk in regulating cell elongation ( Tong et al, 2014 ). The interaction between BR, IAA, and GA on cotton fiber development has been studied in Gossypium hirsutum ( Hu et al, 2011 ). A class of DELLA proteins GhGAII was down-regulated by BR and auxin treatment during cotton fiber initiation and elongation, suggesting its importance in cotton fiber improvement through genetic modulation of phytohormone strategy.…”

Section: Brassinosteroid-gibberellin Crosstalkmentioning

confidence: 99%

“…A class of DELLA proteins GhGAII was down-regulated by BR and auxin treatment during cotton fiber initiation and elongation, suggesting its importance in cotton fiber improvement through genetic modulation of phytohormone strategy. However, the expression of GhGAI1 and GhGAI3 were up-regulated by GA during cotton fiber initiation which is an unessential trait for fiber initiation ( Hu et al, 2011 ) indicating BR-GA crosstalk in regulating cotton fiber development. The synergistic interaction between BR and GA leading to cell expansion during photomorphogenesis has been demonstrated through concurrence of BR-activated BZR1 and GA-inactivated DELLA transcription regulators ( Gallego-Bartolomé et al, 2012 ).…”

Section: Brassinosteroid-gibberellin Crosstalkmentioning

confidence: 99%

Versatile roles of brassinosteroid in plants in the context of its homoeostasis, signaling and crosstalks

2015

View full text Add to dashboard Cite

Brassinosteroids (BRs) are a class of steroidal plant hormones that play diverse roles in plant growth and developmental processes. Recently, the easy availability of biological resources, and development of new molecular tools and approaches have provided the required impetus for deeper understanding of the processes involved in BRs biosynthesis, transport, signaling and degradation pathways. From recent studies it is also evident that BRs interact with other phytohormones such as auxin, cytokinin, ethylene, gibberellin, jasmonic acid, abscisic acid, salicylic acid and polyamine in regulating wide range of physiological and developmental processes in plants. The inputs from these studies are now being linked to the versatile roles of BRs. The present review highlights the conceptual development with regard to BR homeostasis, signaling and its crosstalk with other phytohormones. This information will assist in developing predictive models to modulate various useful traits in plants and address current challenges in agriculture.

show abstract

Brassinosteroids and Auxin Down-Regulate DELLA Genes in Fiber Initiation and Elongation of Cotton

Cited by 20 publications

References 36 publications

Role of GhHDA5 in H3K9 deacetylation and fiber initiation in Gossypium hirsutum

Role of GhHDA5 in H3K9 deacetylation and fiber initiation in Gossypium hirsutum

Mechanisms of signaling crosstalk between brassinosteroids and gibberellins

Versatile roles of brassinosteroid in plants in the context of its homoeostasis, signaling and crosstalks

Contact Info

Product

Resources

About