Brazilian red propolis reduces orange-complex periodontopathogens growing in multispecies biofilms

Brazilian Red Propolis Is as Effective as Amoxicillin in Controlling Red-Complex of Multispecies Subgingival Mature Biofilm In Vitro

“…BRP contains mostly polar compounds, which facilitates its dissolution in aqueous vehicles and sustains the presence of several isoflavones—compounds with documented beneficial properties for humans [ 12 ]. The main relevant pharmacological actions of BRP include antimicrobial [ 13 , 14 , 15 ], anti-inflammatory [ 16 , 17 , 18 ], anti-tumoral [ 19 ], healing [ 20 ], antioxidant and antiparasitic properties [ 15 ].…”

Additive manufacturing of titanium alloy could modify the pathogenic microbial profile: an in vitro study

“…In addition, representative images of the surfaces of each group of specimens were obtained using SEM. 6 Most species were cultured on tryptone soy agar plus 5% sheep's blood under anaerobic conditions (85% nitrogen, 10% carbon dioxide, and 5% hydrogen); P. gingivalis was grown on tryptone soy agar plus yeast extract, 1% hemin, 5% menadione, and 5% sheep blood, and T. forsythia was grown on tryptone soy agar plus yeast extract enriched with 1% hemin, 5% menadione, 5% sheep blood, and 1% N-acetylmuramic acid. After 24 h of growth, all species were transferred to cylindrical tubes with brain heart infusion (BHI) culture medium (Becton Dickinson, Sparks, MD) supplemented with 1% hemin.…”

“…The individual cell suspensions were diluted to obtain a final 15-mL biofilm inoculum with 10 5 cells/mL. 6 Round-shaped titanium discs were placed vertically in a 96-well plate to act as substrate for biofilm formation. There were three groups of titanium discs: G1, which were discs with machined surface (control); G2, which were discs produced via AM of titanium with chemical treatment, and G3, which were discs produced via AM of titanium without chemical treatment.…”

“…In this sense, the present model has an advantage over other biofilm models because it allows the growth of more species. This model could be employed to test therapeutic options 6 and/or to simulate bacterial adhesion to distinct surfaces, as demonstrated in the current study.…”

Evaluation of the antiproliferative activity of red propolis hydroalcoholic extract and its fractions obtained by partition

“…Propolis is a product of honeybee hives, containing mainly beeswax and a resin obtained from various plant sources such as apical buds, young leaves and exudates (Salatino & Salatino 2018). Red propolis, with Dalbergia ecastaphyllum (Daugsch et al, 2008;Silva et al, 2008) and Symphonia globulifera (Ccana-Ccapatinta et al, 2020) as its main botanical sources, has recently stood out as a natural medicinal product due to its various biological properties such as anti-inflammatory (Batista et al 2018), antitumoral (Salatino & Salatino 2018) antioxidant, cytotoxic (de Oliveira Reis et al, 2019), antimicrobial (Miranda et al 2019) activities and healing capacity (Picolotto et al 2019). The biological activity of red propolis is mainly related to isoflavones, which act in synergy with the other compounds.…”