BRCA1 and BRCA2 proteins are expressed in milk fat globules

Bernard‐Gallon, Dominique; Déchelotte, P.; Rio, Pascale; Bignon, Yves‐Jean

doi:10.1002/(sici)1097-0215(19990531)81:5<839::aid-ijc28>3.0.co;2-v

Cited by 6 publications

(3 citation statements)

References 11 publications

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“…2,3 BRCA1 has been variously localised to the nucleus of normal breast epithelial cells, 4 ± 8 to cylinders derived from cytoplasmic structures which anastamose through the nucleus 9 and to the secretory apparatus 10 and associated secretions. 11,12 In addition, it has been reported to both remain in the nucleus 7 and to be present in the cytoplasm of breast cancer cell 4 ± 6 (reviewed in 13,14 ). Some or all of these observations may be reconciled by the hypothesis that BRCA1 transcripts are differentially spliced, 15 ± 17 and that the different mature proteins are directed to specific subcellular sites.…”

Section: Introductionmentioning

confidence: 99%

Laminin-rich extracellular matrix association with mammary epithelial cells suppresses Brca1 expression

O'Connell

Martin

2000

Cell Death Differ

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Brca1 mRNA was detectable in female mouse mammary gland tissue from adult virgins, during pregnancy and early lactation. It was associated with phases of mammary epithelial cell proliferation and differentiation but the pattern of Brca1 expression was dissociable from that of a true differentiation marker, b-casein, by virtue of its significant expression in the virgin gland and termination of its expression in early lactation. In a primary cell culture model, association of a laminin-rich extracellular matrix (ECM) with mammary epithelial cells was required for cell survival and cell differentiation and suppressed Brca1 expression in these cells. ECM-association may significantly contribute to the final restriction in Brca1 expression in the lactating gland in vivo. Interestingly, our results suggest that mammary epithelial cells undergo apoptosis both when expressing and when not expressing Brca1, depending on whether the dying cell populations had been terminally differentiated or not. Cell Death and Differentiation (2000) 7, 360 ± 367.

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Section: Introductionmentioning

confidence: 99%

Laminin-rich extracellular matrix association with mammary epithelial cells suppresses Brca1 expression

O'Connell

Martin

2000

Cell Death Differ

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“…Wilson et al [ 11 ] also reported recently that 17F8 antibody detected BRCA1 nuclear and cytoplasmic stainings in breast specimens according to antibody concentration. We have also reported a nuclear staining pattern with different tissues, notably in child mammary gland [ 12 ] and in lung from a fetus at 19 weeks' gestation [ 13 ], with the 8F7 and 17F8 anti-BRCA1 monoclonal antibodies raised against a glutathione- S -transferase (GST)-BRCA1 fusion protein containing amino acids encoded by a 3´ portion of BRCA1 exon 11 and by a 5´ portion of BRCA1 exon 11, respectively. These antibodies seemed to remain more specific, and they exhibited nuclear staining.…”

Section: Resultsmentioning

confidence: 99%

BRCA1 and BRCA2 protein expressions in an ovotestis of a 46, XX true hermaphrodite

Bernard‐Gallon

Déchelotte²,

Vissac

et al. 2000

Breast Cancer Res

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BRCA1 and BRCA2 breast cancer susceptibility genes encode proteins, the normal cellular functions of which are complex and multiple, and germ-line mutations in individuals predispose both to breast and to ovarian cancer. There is nevertheless substantial evidence linking BRCA1 and BRCA2 to homologous recombination and DNA repair, to transcriptional control and to tissue proliferation. There is controversy regarding the localization of BRCA1 and BRCA2 proteins to either nucleus or cytoplasm and whether the expression is present in premeiotic germ cells or can still be expressed in mitotic spermatogonia. We report herein an immunohistochemical study of BRCA1 and BRCA2 distribution in a rather unsual tissue (an ovotestis), which addresses this issue.

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“…BRCA1 and BRCA2 encode multifunctional protein products implicated in transcriptional regulation, cell cycle control, apoptosis, and DNA-repair pathways (19). The detection of the protein products of the BRCA1 and BRCA2 genes in milk fat globules also suggests a possible role in lactation (20).…”

Section: Hormonal and Genetic Factors In Breast Cancermentioning

confidence: 98%