BRCA1 expression, proliferative and apoptotic activities in ovarian epithelial inclusions

Wang, Yiying; Wang, Yue; Li, Wei; Hong, Shuhui; Zhao, Mingqiu; Zhang, Xi; Zheng, Wenxin

doi:10.1186/s13048-017-0307-6

Cited by 2 publications

(2 citation statements)

References 33 publications

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“…These findings provide evidence to support the conclusion that PRRX1 is a target gene of miR-1307. Up to now, several important genes involved in tumorigenesis had been identified as the target genes of miR-1307, such as BCL2 20 , TYMS 21 , MDM4 32 , SMYD4 23 , DAB2IP 24 , FOXO3A 25 , ING5 26 , and ISM1 33 , indicating critical roles of miR-1307 in cancers.…”

Section: Discussionmentioning

confidence: 99%

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The rs7911488-T allele promotes the growth and metastasis of colorectal cancer through modulating miR-1307/PRRX1

et al. 2020

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We previously discovered that rs7911488T>C in pre-miR-1307 was closely correlated to the risk of colorectal cancer (CRC). However, the roles of rs7911488 in CRC are still largely unknown. Here we explored the roles of rs7911488 in the growth and metastasis of CRC. We firstly generated cell lines SW480-T and SW480-C for stable expression of rs7911488 T-allelic and C-allelic pre-miR-1307, respectively. We subcutaneously grafted the cells into nude mice. We found that SW480-T tumors with high expression of miR-1307 obviously grew faster than the SW480-C tumors. Moreover, liver metastases (5/8) were observed in the mice bearing SW480-T tumors but not the SW480-C tumor-bearing mice. The results from colony formation assays, transwell assays, and wound healing assays demonstrated that the proliferative and metastatic abilities of SW480-T cells were evidently more potent than the SW480-C cells. Then we utilized gene array, real-time PCR, western blotting, and dual-luciferase reporter assays to figure out that miR-1307 directly inhibited PPRX1 expression by binding to its 3′-UTR. Thereafter, we confirmed that the proliferative and metastatic abilities of SW480 and HCT-116 cells were markedly enhanced by miR-1307, but were suppressed by PRRX1. Moreover, the regulatory roles of miR-1307 in the proliferation and metastasis of CRC cells were reversed by PRRX1. Notably, we also found that PRRX1 repressed CRC tumor growth in nude mice. In summary, our current study revealed that rs7911488-T allele led to over-expression of miR-1307, which inhibited PRRX1 and consequently promoted the proliferation and migration of CRC cells. This might offer a novel insight into the progression of CRC.

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Section: Discussionmentioning

confidence: 99%

“…Chen et al provided evidence to support that miR-1307 played a very important role in the progression of liver cancer by targeting DAB2IP 24 . Moreover, studies demonstrated that miR-1307 boosted the progression of prostate cancer by targeting FOXO3A 25 and induced chemoresistance of ovarian cancer cells by inhibiting ING5 26 .…”

Section: Introductionmentioning

confidence: 99%