Breaking up is hard to do – membrane traffic in cytokinesis

Abstract

Centrosomes, centriolin and a potential link to secretory membrane traffic Traffic from the trans-Golgi network (TGN) and the secretory pathway to the furrow and/or midbody of dividing cells is thought

Mutations inCog7affect Golgi structure, meiotic cytokinesis and sperm development duringDrosophilaspermatogenesis

“…Cytokinesis involves a rapid and massive expansion in plasma membrane surface area that strongly depends on endocytic and/or secretory membrane trafficking pathways in several cell systems (Albertson et al, 2005;Boucrot and Kirchhausen, 2007;McKay and Burgess, 2011;Neto et al, 2011;Prekeris and Gould, 2008). Previous studies have demonstrated that spermatocyte cytokinesis is sensitive to Brefeldin A (Robinett et al, 2009) and requires the wild-type function of the Golgi proteins Syntaxin 5 (Xu et al, 2002a), Fws , Fwd (Polevoy et al, 2009) and Brunelleschi (Robinett et al, 2009), indicating a requirement for effective Golgi trafficking.…”

Cytokinesis in Animal Cells

“…Studies in mammalian cells and animal model systems have implicated several components of the secretory and endocytic/recycling machineries in cytokinesis (Prekeris and Gould 2008;Neto et al 2011;Giansanti et al 2012;Tang 2012). A proteomics-based analysis of purified midbodies isolated from Chinese hamster ovary cells revealed that the largest proportion of midbody proteins had a known role in secretory and membrane trafficking; the requirement for cytokinesis of many of the homologs was confirmed by RNA interference in C. elegans (Skop et al 2004).…”

“…Endocytic and recycling proteins are also key players in mediating both furrow ingression and abscission (Prekeris and Gould 2008).…”

Making the Final Cut - The Role of Endosomes During Mitotic Cell Division

“…The last step of cell division is the physical separation of two daughter cells via a process known as cytokinesis (Barr and Gruneberg, 2007;Prekeris and Gould, 2008). After replication of the genetic material, the mother cell divides by the formation of a cleavage furrow that constricts the cytoplasm, thus leaving two daughter cells connected by a thin intracellular bridge (ICB).…”

“…While earlier studies thought this abscission event was simply a continuation of the constriction placed on the cleavage furrow by the actomyosin contractile ring, it was later discovered that abscission is a highly complex and organized event consisting of much more than a simple actin and non-muscle myosin constricting ring. Endocytic membrane transport, ESCRT protein complex function, and cytoskeletal reorganization were all shown to contribute to cytokinesis and abscission (Barr and Gruneberg, 2007;Prekeris and Gould, 2008). The goal of this review is provide an overview the newest advances in our understanding about the dynamics and roles of endosomal transport during cytoskeletal re-arrangements and ESCRT complex assembly throughout cytokinesis.…”