Breakthrough performance of high-capacity membrane adsorbers in protein chromatography

Gebauer, Klaus; Thömmes, Jörg; Kula, Maria‐Regina

doi:10.1016/s0009-2509(96)00426-5

Cited by 94 publications

(42 citation statements)

References 38 publications

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“…If the shape of the isotherm is rectangular the total binding capacity is independent from the solute concentration [8]. This favorable shape has often been reported for ion exchange resins and is one of the reason for almost the highest binding capacities reported [9].…”

Section: Equilibrium Binding Capacitiesmentioning

confidence: 78%

Properties and Characterization of High Capacity Resins for Biochromatography

Müller¹

2005

Chem Eng & Technol

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At the moment ion exchanger resins display the highest binding capacities for proteins. Polymeric surface modification methods utilize the full pore space and allow to guarantee high capacities for small as well as for large proteins. Capacity maximization is also possible by pore size optimization fitting the pore size to the molecule size without loosing a lot of dynamic binding capacity under current process conditions. Further improvements of resins can be achieved by optimizing the pore structure and pore volume, which could enhance the mass transfer and so the dynamic binding capacity.

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Section: Equilibrium Binding Capacitiesmentioning

confidence: 78%

Properties and Characterization of High Capacity Resins for Biochromatography

Müller¹

2005

Chem Eng & Technol

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“…A utilização de suportes do tipo membrana, utilizando polímeros sintéticos ou naturais, tais como celulose, quitosana, poliamida, poli (álcool vinílico), poli (metacrilato de hidroxietila) e polisulfona têm sido investigadas (Gebauer et al, 1996;Gan et al, 2000;Ruckenstein, 2001;Garipcan et al, 2001;Zou et al, 2001;Vyas et al, 2001;Srivastava et al, 2012;Lv et al, 2013).…”

Section: Introductionunclassified

Extração, Precipitação E Reticulação Da Mucilagem De Jaracatiá (Carica Quercifolia (A. St.-Hil) Hieron) Para Uso Como Adsorvente De Proteínas

Heidemann¹,

Faccio²,

Quadri³

et al. 2015

Anais Do XX Congresso Brasileiro De Engenharia Química

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RESUMO -A mucilagem é composta quimicamente por água, pectinas, açúcares e ácidos orgânicos, tornando-se viscosa na presença de água. O objetivo deste estudo foi a reticulação da mucilagem de jaracatiá (Carica quercifolia (A. St.-Hil.) Hieron) precipitada, a qual é composta por polissacarídeos naturais, para uso como adsorvente de proteínas, aproveitando assim os produtos da biodiversidade brasileira. Após a extração e precipitação com etanol, a mucilagem foi reticulada com epicloridrina. O rendimento da mucilagem de jaracatiá precipitada e da reticulação, os grupos funcionais antes e após a reticulação e a capacidade de adsorção do reticulado utilizando papaína foram avaliados. O rendimento da mucilagem precipitada em relação a massa de polpa do fruto foi de 1-1,5% e, após a reticulação o rendimento ficou em torno de 20% de mucilagem reticulada em relação à massa inicial de mucilagem precipitada. Os espectros FTIR mostraram que houveram mudanças nas bandas características com a reticulação. A capacidade da adsorção de papaína mostrou-se satisfatória, em torno de 50%.

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“…In many studies, attempts have been made to quantitatively model different adsorption mechanisms [12], different binding isotherms [14,24] and the entire membrane chromatographic process, i.e., the mass transfer coupled with binding kinetics [11,13]. In these approaches, dead volumes in membrane chromatography systems are traditionally modeled by one plug flow reactor (PFR) and one continuous stirred tank reactor (CSTR) in series [10,11], or by lumping the overall peak broadening and tailing effects into a fixed volume of a mixing cell [15].…”

Section: Introductionmentioning

confidence: 99%

Model Based Quantification of Internal Flow Distributions from Breakthrough Curves of Flat Sheet Membrane Chromatography Modules

2010

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A novel model for quantifying radial flow distributions in flat sheet membrane chromatography modules under non-binding conditions is presented and applied for the practical analysis of two modules. The proposed model partitions the total void volume of the chromatography module into zones that are considered homogeneous with respect to flow velocity. The corresponding solute concentrations are time variant, but also spatially homogeneous within each zone. The model is mathematically represented and analytically solved as a network of continuously stirred tank reactors (CSTR). An additional plug flow reactor (PFR) is connected in series with the CSTR network in order to account for a time-lag that is not associated with the system dispersion. The capability of the model to describe experimental breakthrough data is compared to the frequently applied standard model for extra-membrane system dispersion, which consists of a single CSTR in series with a PFR. Non-binding conditions are deliberately chosen for studying the impact of module geometries on breakthrough curves separately from chromatographic membrane performance. The commercial CIM module and a custom designed cell (Scell) are studied with acetone and lysozyme as test tracers at varied flow rates and for various membrane pore sizes under non-binding conditions. In all studied cases, the proposed model fits the measured breakthrough curves better than the standard model. Moreover, the minimal number of radial flow zones that are required to accurately describe observed breakthrough curves and the estimated flow fractions through these zones provide valuable information for the analysis and optimization of internal module designs.

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Breakthrough performance of high-capacity membrane adsorbers in protein chromatography

Cited by 94 publications

References 38 publications

Properties and Characterization of High Capacity Resins for Biochromatography

Properties and Characterization of High Capacity Resins for Biochromatography

Extração, Precipitação E Reticulação Da Mucilagem De Jaracatiá (Carica Quercifolia (A. St.-Hil) Hieron) Para Uso Como Adsorvente De Proteínas

Model Based Quantification of Internal Flow Distributions from Breakthrough Curves of Flat Sheet Membrane Chromatography Modules

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