Breed Discrimination Using DNA Markers Derived from AFLP in Japanese Beef Cattle

Sasazaki, Shinji; Imada, T.; Mutoh, H.; Yoshizawa, K.; Mannen, Hideyuki

doi:10.5713/ajas.2006.1106

Cited by 12 publications

(16 citation statements)

References 8 publications

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“…The annealing temperature of all the 11 markers ranged from 59 to 65 • C whereas the product sizes of given markers ranged from 99 to 570 bp. The product sizes were different from the previously reported studies (Sasazaki et al, 2004(Sasazaki et al, , 2006. This difference could be credited to a difference in breeds.…”

Section: Aflp Markerscontrasting

confidence: 72%

“…Allelic frequencies were estimated as given in Table 5. The frequency of allele 1 ranged from 0.073 to 1.00 in the Cholistani population and 0.083 to 1.00 in the case of the cross- probability of misjudgment was higher in the current study as compared to previous reports (Sasazaki et al, 2004(Sasazaki et al, , 2006. This difference could again be because of the breed difference.…”

Section: Allelic Frequenciescontrasting

confidence: 63%

“…The present research was aimed to develop specific molecular markers for the discrimination of Cholistani cattle from crossbred population. The approach used was AFLP, previously applied by Sasazaki et al (2006) for the discrimination between Japanese black and crossbred populations.…”

Section: Resultsmentioning

confidence: 99%

“…In the current study we adapted AFLP markers for breed documentation since these markers are more informative (Sasazaki et al, 2006). The comprehensive information on each of the markers used, including their forward as well as reverse primers, annealing temperatures, product size and pertinent mutation, are given in Table 2.…”

Section: Aflp Markersmentioning

confidence: 99%

“…Various molecular methods have been used to identify breeds among different farm animal species over the period of time, e.g., microsatellite markers in dogs (Koskinen, 2003), microsatellite markers in goats (Iquebal et al, 2013), microsatellite markers in cattle (Rogberg-Munoz et al, 2014), allele-specific polymerase chain reaction in chicken (Choi et al, 2007), amplified fragment length polymorphism (AFLP) in cattle (Ajmone-Marsan et al, 1997;Sasazaki et al, 2004Sasazaki et al, , 2006 and single nucleotide polymorphism (SNP) chips in cattle (Cooper et al, 2014(Cooper et al, , 2016Suekawa et al, 2010). However, AFLP markers have been extensively applied in DNA fingerprinting (Ajmone-Marsan et al, 1997;Vos et al, 1995), genetic distance analysis (Ajmone-Marsan et al, 2002), quantitative trait loci (QTL) mapping (Milanesi et al, 2008), linkage mapping (Huang et al, 2009) and lastly, of course, breed identification (Negrini et al, 2007a, b;Sasazaki et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Development of amplified fragment length polymorphism (AFLP) markers for the identification of Cholistani cattle

et al. 2018

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Abstract. The identification issue of livestock can be resolved by using molecular identification tools that are acceptable to preserve and maintain pure breeds worldwide. The application of a molecular identification methodology is more important for developing nations, e.g., Pakistan, where uncontrolled crossbreeding has become a common practice and the import of exotic animals and germplasm is ever increasing. This presents a risk to local breeds as also stated by the FAO. Therefore, the current study was designed to develop standard molecular markers for Cholistani cattle to ascertain their purity for breeding purpose. In this study 50 and 48 unrelated males were sampled for Cholistani and each crossbred cattle, respectively. Candidate molecular markers present in Cholistani but absent in crossbred cattle and vice versa were detected using the amplified fragment length polymorphism (AFLP) method. Eleven markers were developed and were converted to single nucleotide polymorphism (SNP) markers for genotyping. The allele frequencies in both breeds were determined for discrimination ability using polymerase-chain-reaction–restriction-fragment-polymorphism (PCR-AFLP). The probability of identifying the Cholistani breed was 0.905 and the probability of misjudgment was 0.073 using a panel of markers. The identified markers can ascertain the breed purity and are likely to extend the facility for breed purity testing before entering into a genetic improvement program in the country.

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Section: Aflp Markerscontrasting

confidence: 72%

Section: Allelic Frequenciescontrasting

confidence: 63%

Section: Resultsmentioning

confidence: 99%

Section: Aflp Markersmentioning

confidence: 99%