Breeding for Nutritional Quality

Juhász, Ana Cristina Pinto; Ciabotti, Sueli; Teixeira, Lilian Cristina Andrade de Araújo

doi:10.1007/978-3-319-57433-2_20

Cited by 2 publications

(1 citation statement)

References 28 publications

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“…The cultivar UFV TN 105 AP designated as “105 AP” is a variety of early cycle that was developed by the Soybean Breeding Program of the Biotechnology Institute of the Universidade Federal de Viçosa (BIOAGRO/UFV). In this genotype, three isoforms coding for LOX1, LOX2 e LOX3 lipoxygenases were eliminated [ 12 , 17 ]. The cultivar IAC 17 is a variety of early cycle [ 18 – 20 ], of genealogy D 72-9601-1 x ‘IAC 8’ and considered a resistant genotype to herbivory against A .…”

Section: Methodsmentioning

confidence: 99%

Broad range flavonoid profiling by LC/MS of soybean genotypes contrasting for resistance to Anticarsia gemmatalis (Lepidoptera: Noctuidae)

Gómez¹,

Vital

Oliveira³

et al. 2018

PLoS ONE

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Attack by herbivores is a major biotic stress limiting the soybean crop production. Plant defenses against caterpillars include the production of secondary metabolites such as flavonoids, which constitute a diverse group of plant secondary metabolites. Thus, a more discriminate metabolic profiling between genotypes are important for a more comprehensive and reliable characterization of soybean resistance. Therefore, in this study a non-targeted LC/MS-based for analysis of flavonoid profiles of soybean genotypes contrasting to the resistance to A. gemmatalis was applied. Clustering analysis revealed profiles highly distinct between the susceptible UFV 105 AP and the resistant IAC 17 genotypes. This comparative approach enables to identify directly from leaf extract some new compounds related to resistance, some of which were present in higher abundance specifically in the IAC 17 genotype: four Quercetin conjugates, Rutin (Quercetin 3-O-Rutinoside), Quercetin-3,7-O- di-glucoside, Quercetin-3-O-rhamnosylglycoside-7-O-glucoside and Quercetin-3-O-rhamnopyranosyl-glucopyranoside-rhamnopyranoside; two Genistein conjugates, Genistein-7-O-diglucoside-dimalonylated and Genistein-7-O-6-O-malonylglucoside; and one Daidzein conjugate, Daidzein-7-O-Glucoside-malonate. The most abundant flavonoid glycoconjugates in soybean leaves belongs to Quercetin and Kaempferol classes. However, only one from the identified compounds was classified as a Kaempferol. The Kaempferol-3-O-L-rhamnopyranosyl-glucopyranoside showed high abundance in the resistant genotype IAC 17. The metabolic profiles generated by LC/MS allowed the reconstruction of the flavonoid biosynthetic pathways, which revealed a constitutive character for herbivory resistance in the resistant genotype IAC-17 and a metabolic regulation for the rechanneling of Quercetin, Kaempferol and Genistein conjugates in soybean. Highest relative abundances were detected for glyconjugates, such as Rutin, Quercetin 3-O-rhamnosylglycoside-7-O-glucoside and Quercitin-3-O-rhamnopyranosyl-glucopyranoside-rhamnopyranoside in the leaves of the resistant genotype.

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Section: Methodsmentioning

confidence: 99%