Breeding melon (Cucumis melo) with resistance to powdery mildew and downy mildew

Cui, Lei; Siskos, Lampros; Wang, Chen; Schouten, Henk J.; Visser, R.G.F.; Bai, Yuling

doi:10.1016/j.hpj.2022.07.006

Cited by 22 publications

(17 citation statements)

References 155 publications

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“…The TMV resistance protein N-like, a TIR-NBS-LRR gene possessing homology with resistance genes [ 46 ], was frequently annotated (seven of the annotated genes in the candidate interval). As the dominant resistances of powdery mildew identified so far have been easily broken by newly emerging races [ 47 ], it has been postulated that many of the QTLs detected could encode PRRs that would be manipulated by the secreted fungal effectors [ 48 , 49 ]. It has recently been proposed that several dominant resistances against powdery mildew in melon could be caused by missense mutations in different LRR-receptor like kinases and TIR-NBS-LRR genes [ 19 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

Advanced Genetic Studies on Powdery Mildew Resistance in TGR-1551

López-Martín

Pérez‐de‐Castro

Picó

2022

IJMS

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Cucurbits powdery mildew (CPM) is one of the main limiting factors of melon cultivation worldwide. Resistance to races 1, 2, and 5 has been reported in the African accession TGR-1551, whose resistance is controlled by a dominant–recessive epistasis. The dominant and recessive quantitative trail loci (QTL) have previously been located in chromosomes 5 and 12, respectively. We used several densely genotyped BC3 families derived from the cross between TGR-1551 and the susceptible cultivar ‘Bola de Oro’ to finely map these resistance regions. The further phenotyping and genotyping of the selected BC5, BC5S1, BC5S2, BC4S1, BC4xPS, and (BC4xPS) S1 offspring allowed for the narrowing of the candidate intervals to a 250 and 381 kb region in chromosomes 5 and 12, respectively. Moreover, the temperature effect over the resistance provided by the dominant gene has been confirmed. High resolution melting markers (HRM) were tightly linked to both resistance regions and will be useful in marker-assisted selection programs. Candidate R genes with variants between parents that caused a potential modifier impact on the protein function were identified within both intervals. These candidate genes provide targets for future functional analyses to better understand the resistance to powdery mildew in melons.

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Section: Discussionmentioning

confidence: 99%

Advanced Genetic Studies on Powdery Mildew Resistance in TGR-1551

López-Martín

Pérez‐de‐Castro

Picó

2022

IJMS

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“…Development of markers, especially the high throughput ones, is essential for mass screening and speed breeding these days (Thomson 2014;Watson et al 2018;Wanga et al 2021). Several SSR markers: CMBR150 and CMBR111 (Fukino et al 2008), CMN1-38 (Fukino et al 2007), CMBR120 (Kim et al 2016b), and TJ29 (Gonzalo et al 2005) CMCT170b andCMMS30-3 (ÜNLÜ et al 2022); SRAP marker: Pm-8 (Liu et al 2010) and CAPs markers: CAPS-Dde I (Zhang et al 2013), andPM2-CAPS andPM5-CAPS (Yuste-Lisbona et al 2011) were linked with linked to PM resistance against specific races (Cui et al 2022). These markers were mainly used to develop linkage maps and are of low throughput.…”

Section: Discussionmentioning

confidence: 99%

Inheritance of Resistance to Race 5 of Powdery Mildew Fungus Podosphaera xanthii in Melon and Development of Race 5-Specific High Resolution Melting Markers

Hong

Hossain

Jung

et al. 2022

Plant Breed. Biotech.

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Powdery mildew (PM), caused by the biotrophic fungus Podosphaera xanthii, drastically reduces the yield and quality of melon (Cucumis melo L.). Knowledge of the genetic control and high throughput molecular markers linked with resistance against this disease are essential for breeding programs. The bioassay study of the F1 and F2 populations derived from the parents, 'PMR 5' (♂) and 'SCNU1154' (♀) revealed a monogenic dominant nature of resistance to the devastating race, race 5. Besides, we developed three SNP based high resolution melting markers, PMm-HRM-1, PMm-HRM-2, and PMm-HRM-3, based on the previously identified SNPs on chromosome 12 and validated them using 8 melon lines and 137 F2 populations. Among these, the SNP of marker PMm-HRM-1 causes a missense mutation in the LRR region of MELO3C002393 and we were able to distinguish the resistant vs susceptible genotypes from eight diverse melon accessions and the segregating F2 population with more than 90% genotyping efficiency. The other two markers were based on intergenic SNPs and had more than 80% genotyping efficiency in F2 population. These markers will be helpful to melon breeders to develop melon cultivars resistant to P. xanthii race 5 via marker assisted breeding programs.

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“…31 A recent comprehensive review article on melon breeding for developing hybrids with resistance to powdery mildew and downy mildew has been discussed about the valuable melon germplasm accessions that can be used in resistance breeding. 32 The multiple resistance genes are found in various accessions of C. melo var. cantalupensis (PMR 45, PMR 5, PMR 6, and WMR 2) and C. melo var.…”

Section: Disease Resistance Of Melonmentioning

confidence: 99%

“…cantalupensis (PMR 45, PMR 5, PMR 6, and WMR 2) and C. melo var. momordica (PI 124111, PI 124112, and PI 414723), hence these accessions can be used as potential resistance donors in resistance breeding programmes 32 (Fig. 3).…”

Section: Disease Resistance Of Melonmentioning

confidence: 99%

Genetic Diversity and Molecular Breeding of Melon (Cucumis melo L.): A review

Komala

Kuni

2023

Curr Agri Res Jour

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Cucumis melo L. is an important horticultural crop that is widely cultivated for its edible fruit. The breeding of melon crop in order to improve its qualitative and quantitative yield is the objective of the current research. The recent melon breeding majorly focused on identification of novel genes, novel QTLs, and differentially expressed genes among the various populations. The publication of melon genome sequence in 2012, substantially accelerated the genetic dissection of candidate genes responsible for various desirable traits such as yield and yield contributing traits, fruit quality, disease resistance (Powdery mildew and Downey mildew resistance), salinity and temperature stress, and the genes involving in melon plant physiology. The molecular biology approaches such as whole-genome resequencing, bulked segregant analysis sequencing and the transcriptome analysis have been widely used to explore the genetic basis for the observed desirable phenotypic characteristics of the melon accessions in the germplasm to further aid in future molecular breeding of melon. Here, we summarized such research reports and categorized them under various sub sections. Overall, this current review presents the overview of very recent advances in melon crop improvement and developments in melon molecular breeding approaches as well as it provides the future directions for melon breeding. It would probably provide recent updates on melon crop improvement and help melon breeders to plan for their future breeding programmes.

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Breeding melon (Cucumis melo) with resistance to powdery mildew and downy mildew

Cited by 22 publications

References 155 publications

Advanced Genetic Studies on Powdery Mildew Resistance in TGR-1551

Advanced Genetic Studies on Powdery Mildew Resistance in TGR-1551

Inheritance of Resistance to Race 5 of Powdery Mildew Fungus Podosphaera xanthii in Melon and Development of Race 5-Specific High Resolution Melting Markers

Genetic Diversity and Molecular Breeding of Melon (Cucumis melo L.): A review

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